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リコンビナントRabMAb

HRP Anti-VDAC1/Porin + VDAC2 抗体 [EPR10852(B)] - Mitochondrial Loading Control (ab185063)

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Western blot - HRP Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab185063)
  • HRP Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab185063)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR10852(B)] to VDAC1/Porin + VDAC2 - Mitochondrial Loading Control
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Conjugation: HRP

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 APC Carrier Free PE Unconjugated

製品の概要

  • 製品名

    HRP Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control

  • 製品の詳細

    HRP Rabbit monoclonal [EPR10852(B)] to VDAC1/Porin + VDAC2 - Mitochondrial Loading Control

  • 由来種

    Rabbit

  • 標識

    HRP

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HepG2 and Jurkat whole cell and Human Kidney, Mouse Kidney and Rat Kidney tissue lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab185063の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).
特記事項
WB
1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).

ターゲット情報

画像

  • Western blot - HRP Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab185063)
    Western blot - HRP Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab185063)
    All lanes : HRP Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab185063) at 1/5000 dilution

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa whole cell lysate (ab150035)
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : Kidney (Mouse) Tissue Lysate
    Lane 5 : Kidney (Rat) Tissue Lysate
    Lane 6 : Kidney (Human) Tissue Lysate - adult normal tissue

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 31 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?


    Exposure time: 4 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab185063 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • HRP Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab185063)
    HRP Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab185063)

参考文献 (1)

ab185063 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab185063 は 1 報の論文で使用されています。

  • Wang Q  et al. Dysregulation of mitochondrial complexes and dynamics by chronic cigarette smoke exposure Utilizing MitoQC reporter mice. Mitochondrion 63:43-50 (2022). PubMed: 35032706

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