HRP Anti-T7 tag® 抗体 (ab19291)
Key features and details
- HRP Rabbit polyclonal to T7 tag®
- Suitable for: ELISA, ICC, WB
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG
製品の概要
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製品名
HRP Anti-T7 tag® antibody
T7 tag® 一次抗体 製品一覧 -
製品の詳細
HRP Rabbit polyclonal to T7 tag® -
由来種
Rabbit -
標識
HRP -
アプリケーション
適用あり: ELISA, ICC, WBmore details -
種交差性
交差種: Species independent -
免疫原
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特記事項
Molar enzyme/antibody protein ratio is 4:1.
T7-TAG® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.8
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antibody was isolated by affinity chromatography using the peptide immobilized on solid support and conjugated to horseradish peroxidase (HRP). -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab19291の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ELISA | ||
ICC | ||
WB |
特記事項 |
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ICC: 1/200 - 1/500.
WB: 1/1000 - 1/30000. Colorimetric detection: 1:1000 - 1:10000, Chemiluminescent detection: 1:1000 - 1:30000
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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関連性
The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins. -
別名
- 10 antibody
- Major capsid protein 10A antibody
- Major capsid protein antibody
see all
画像
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All lanes : HRP Anti-T7 tag® antibody (ab19291) at 1/5000 dilution
Lane 1 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.2 µg
Lane 2 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.1 µg
Lane 3 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.05 µg
Predicted band size: 37 kDa
Exposure time: 3 minutesDetected by chemiluminescence.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab19291 は 1 報の論文で使用されています。
- Pandey N et al. Topoisomerase II SUMOylation activates a metaphase checkpoint via Haspin and Aurora B kinases. J Cell Biol 219:N/A (2020). PubMed: 31712254