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HRP Anti-Respiratory Syncytial Virus 抗体 (ab20686)

Submit a review Q&A (4)References (3)

Key features and details

  • HRP Goat polyclonal to Respiratory Syncytial Virus
  • Suitable for: IHC-P, ELISA, Neutralising
  • Reacts with: Respiratory syncytial virus
  • Conjugation: HRP
  • Isotype: IgG

製品の概要

  • 製品名

    HRP Anti-Respiratory Syncytial Virus antibody
    Respiratory Syncytial Virus 一次抗体 製品一覧

  • 製品の詳細

    HRP Goat polyclonal to Respiratory Syncytial Virus

  • 由来種

    Goat

  • 標識

    HRP

  • アプリケーション

    適用あり: IHC-P, ELISA, Neutralisingmore details

  • 種交差性

    交差種: Respiratory syncytial virus

  • 免疫原

    Tissue, cells or virus corresponding to Respiratory Syncytial Virus. Human Respiratory Syncytial Virus isolate.

  • 特記事項

    Covalently coupled to a highly purified preparation of Horseradish Peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is removed.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab20686の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent dilution.
ELISA
1/200 - 1/1000.
Neutralising
1/256.
特記事項
IHC-P
Use at an assay dependent dilution.
ELISA
1/200 - 1/1000.
Neutralising
1/256.

ターゲット情報

  • 関連性

    Respiratory syncytial virus (RSV) is a major cause of respiratory illness in young children. RSV infection produces a variety of signs and symptoms involving different areas of the respiratory tract, from the nose to the lungs. RSV is a negative sense, enveloped RNA virus. The virion is variable in shape and size with average diameter of between 120 and 300 nm. The 63 kD RSV fusion protein of the RSS 2 strain (subtype A) directs fusion of viral and cellular membranes, results in viral penetration, and can direct fusion of infected cells with adjoining cells, resulting in the formation of syncytia or multi nucleated giant cells.

  • 細胞内局在

    Virion. Host cytoplasm

  • 参照データベース

    • 別名

      • RS virus antibody
      • RSV antibody

    参考文献 (3)

    ab20686 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab20686 は 3 報の論文で使用されています。

    • Pavot V  et al. A novel vaccine adjuvant based on straight polyacrylate potentiates vaccine-induced humoral and cellular immunity in cynomolgus macaques. Hum Vaccin Immunother 17:2336-2348 (2021). PubMed: 33427044
    • Jones HG  et al. Structural basis for recognition of the central conserved region of RSV G by neutralizing human antibodies. PLoS Pathog 14:e1006935 (2018). PubMed: 29509814
    • Groppo R  et al. Effect of genetic background and delivery route on the preclinical properties of a live attenuated RSV vaccine. PLoS One 13:e0199452 (2018). PubMed: 29920563

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-4 of 4 Abreviews or Q&A

    Question

    Is the antibody ab20686 specific to RSV subtype A or B?

    Read More
    Answer

    ab20686 was developed using an "A" strain of RSV. Even though it reacts with both A and B strains, its reactivity is much stronger for A strains and weaker for B strains.

    Read More

    Question

    Thank you for replying back. My cells are sitting in 6 well plates, they are cell lines infected with RSV virus and I essentially want to stain the viral plaques for verification.

    After reading the protocol, I understand that this is not the DAB kit which will be solution A and B, am I right.

    Read More
    Answer

    Thank you for your reply.

    You are correct that the DAB enhancer is not the solution with A & B components. It is to be used in conjunction with a DAB chromogen.

    If there is anything else I can help you with, please let me know.

    Read More

    Question

    Hi I have one more question ? what is The DAB Enhancer and what is the protocol for immunostaining if using your antibody for cells that have been fixed in formalin or methanol( non frozen).

    Read More
    Answer

    Thank you for contacting us.

    The DAB enhancement reagent is for use with DAB chromogens. It can be used as an auxiliary step for richer, chocolate-brown DAB results.


    I have also attached our recommended protocol for ICC staining as you have requested.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    I have one more question, The antibody is HRP conjugated so you can use for direct immunostaining. You dont need a secondary antibody.Can your technical department confirm this. Also can you confirm if these 2 items are in stock and when will be they delivered. Thanks

    Read More
    Answer

    Thank you for contacting Abcam.

    AB20686 is an HRP conjugated product and can be used for direct immunostaining. Use of a secondary antibody is un-necessary.

    We have 8 in stock as of Thurday 4pm Pacific time.



    I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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