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AB207635

HRP Anti-PTP1B 抗体 [EP1841Y]

HRP Anti-PTP1B antibody [EP1841Y]

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(1 Publication)

Rabbit Recombinant Monoclonal PTP1B antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

別名を表示する

PTP1B, PTPN1, Tyrosine-protein phosphatase non-receptor type 1, Protein-tyrosine phosphatase 1B, PTP-1B

1 Images
Western blot - HRP Anti-PTP1B antibody [EP1841Y] (AB207635)
  • WB

Lab

Western blot - HRP Anti-PTP1B antibody [EP1841Y] (AB207635)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab207635 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-PTP1B antibody [EP1841Y] (ab207635) at 1/5000 dilution

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 4:

Wild-type HAP1 cell lysate at 20 µg

Lane 5:

PTPB1 knockout HAP1 cell lysate at 20 µg

Predicted band size: 50 kDa

Observed band size: 45 kDa

true

Exposure time: 20min

関連する標識済み抗体及び組成の異なる製品 (2)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP1841Y

アイソタイプ

IgG

標識

HRP

励起波長/蛍光波長
キャリアフリー

No

交差種

Human, Human

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification
バッファー組成
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Protein tyrosine phosphatase 1B (PTP1B) is an enzyme involved in the dephosphorylation of tyrosine residues in proteins. Known also as PTPN1 this protein has a molecular weight of approximately 50 kDa. PTP1B is widely expressed in tissues but is highly present in the liver muscle and adipose tissue. It plays a critical role in regulating signal transduction pathways by removing phosphate groups from phosphotyrosine residues on target proteins. This action generally serves as a 'turn-off' switch for various signaling pathways.
Biological function summary

PTP1B plays important roles in glucose and lipid metabolism. It is not generally considered a part of a complex functioning instead as a singular enzyme. Its main action involves the negative regulation of the insulin signaling pathway by dephosphorylating the insulin receptor as well as its associated substrates. This regulation helps maintain normal levels of insulin sensitivity influencing cellular glucose uptake and metabolism.

Pathways

PTP1B has important functions in the insulin and leptin signaling pathways. It has a regulatory influence in these pathways affecting key metabolic processes. In the insulin signaling pathway PTP1B closely interacts with the insulin receptor as well as with insulin receptor substrates (IRS). Its dephosphorylating activity can lead to decreased insulin sensitivity. In the leptin pathway PTP1B modulates leptin signaling by dephosphorylating the Janus kinase 2 (JAK2) another protein involved in the control of energy balance and metabolism.

Excessive activity of PTP1B links with disorders such as type 2 diabetes and obesity. Overactivity of PTP1B can lead to insulin resistance contributing to the pathogenesis of type 2 diabetes. In obesity PTP1B may interfere with the normal activity of leptin a hormone critical for regulating energy balance. Its interaction with the insulin receptor and JAK2 makes PTP1B a target for therapeutic interventions aimed at improving insulin sensitivity and energy homeostasis.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.
See full target information PTPN1
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

BMC geriatrics 25:645 PubMed40836274

2025

AIMP1 exerts hearing protection role in age related hearing loss mice by regulating SIRT1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Haisen Peng,Jiali Liu,Yuehui Liu,Chunhua Li,Zhilin Zhang,Shuihua Hu,Wen Xie
View all publications
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代替製品

Primary Antibodies

AB244207

Anti-PTP1B antibody [EPR22474]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody [EPR22474] (AB244207)

  • 0
  • 0
Primary Antibodies

AB76239

Anti-PTP1B (phospho S378) antibody [EP1840Y]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B (phospho S378) antibody [EP1840Y] (AB76239)

  • 0
  • 0
Primary Antibodies

AB232050

Anti-PTP1B antibody [EPR1836(2)] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Western blot - Anti-PTP1B antibody [EPR1836(2)] - BSA and Azide free (AB232050)

  • 0
  • 0
Primary Antibodies

AB133244

Anti-PTP1B antibody [EPR1836(2)]

RabMAb|Recombinant|KO Validated

Western blot - Anti-PTP1B antibody [EPR1836(2)] (AB133244)

  • 5
  • 1
Primary Antibodies

AB245984

Anti-PTP1B antibody [EPR22474] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody [EPR22474] - BSA and Azide free (AB245984)

  • 0
  • 0

組合せ製品

Assay Kits

AB139465

PTP1B Inhibitor Screening Assay Kit

Functional Studies - PTP1B Inhibitor Screening Assay Kit (AB139465)

  • 0
  • 0
Biochemicals

AB120358

AICAR (Acadesine/AICA riboside), AMPK activator

Functional Studies - AICAR (Acadesine/AICA riboside), AMPK activator (AB120358)

  • 0
  • 0
Assay Kits

AB139465

PTP1B Inhibitor Screening Assay Kit

Functional Studies - PTP1B Inhibitor Screening Assay Kit (AB139465)

  • 0
  • 0
Proteins & Peptides

AB51277

Recombinant human PTP1B protein (Active) (Tag Free)

SDS-PAGE - Recombinant human PTP1B protein (Active) (Tag Free) (AB51277)

  • 0
  • 0
Primary Antibodies

AB129070

Anti-PTP1B + TCPTP antibody [EPR6712]

RabMAb|Recombinant|Trial Size

Flow Cytometry (Intracellular) - Anti-PTP1B + TCPTP antibody [EPR6712] (AB129070)

  • 0
  • 0

Abcam product promise

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