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HRP Anti-polyHistidine 抗体 [HIS-1] (ab49781)

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Key features and details

  • HRP Mouse monoclonal [HIS-1] to polyHistidine
  • Suitable for: ELISA, Dot blot, WB
  • Reacts with: Species independent
  • Conjugation: HRP
  • Isotype: IgG2a

Conjugates logo Related conjugates and formulations

Alkaline Phosphatase Unconjugated

製品の概要

  • 製品名

    HRP Anti-polyHistidine antibody [HIS-1]

  • 製品の詳細

    HRP Mouse monoclonal [HIS-1] to polyHistidine

  • 由来種

    Mouse

  • 標識

    HRP

  • アプリケーション

    適用あり: ELISA, Dot blot, WBmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    Recombinant full length protein (6x HIS-tag) corresponding to polyHistidine.

  • ポジティブ･コントロール

    • Bacteria lysates expressing a recombinant Histidine tagged fusion protein.

  • 特記事項

    Monoclonal antibody reacting specifically with polyHistidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidine tagged fusion protein expressing vectors.

    The conjugate was prepared by conjugation of HRP to Mouse monoclonal anti-polyHistidine purified from ascites fluid of the HIS-1 hybridoma. Molar ratio Ab/Enzyme: 0.8 to 1.5 Enzyme activity: at least 400 U/ml

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...

  • 精製度

    IgG fraction

  • 特記事項（精製）

    Purified Immunoglobulin

  • 一次抗体 備考

    Monoclonal antibody reacting specifically with polyHistidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidine tagged fusion protein expressing vectors.

  • ポリ/モノ

    モノクローナル

  • クローン名

    HIS-1

  • アイソタイプ

    IgG2a

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab49781の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
Use at an assay dependent concentration.
Dot blot
1/2000.
WB
1/2000.
特記事項
ELISA
Use at an assay dependent concentration.
Dot blot
1/2000.
WB
1/2000.

ターゲット情報

  • 関連性

    Recombinant DNA technology enables the attachment of genes of interest to specific sequences or genes that can provide 'affinity handles' (tags) designed to enable the selective identification of the protein of interest. These sequences of tails or tags are genetically engineered away from the protein active site, by insertion at the N or C terminus. It has been reported that the addition of a consecutive Histidine amino acid residue tail creates a stable fusion product that does not appear to interfere with the bioactivity of the protein or with the biodistribution of the Histidine tagged product.

参考文献 (3)

ab49781 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab49781 は 3 報の論文で使用されています。

  • Moore CE  et al. RFWD3 promotes ZRANB3 recruitment to regulate the remodeling of stalled replication forks. J Cell Biol 222:N/A (2023). PubMed: 37036693
  • Shin J  et al. Characterization of a single chain variable fragment of nivolumab that targets PD-1 and blocks PD-L1 binding. Protein Expr Purif 177:105766 (2021). PubMed: 32987122
  • Kim YJ  et al. Molecular and Structural Characterization of the Tegumental 20.6-kDa Protein in Clonorchis sinensis as a Potential Druggable Target. Int J Mol Sci 18:N/A (2017). PubMed: 28273846

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