AB16389

HRP Anti-Phosphotyrosine 抗体 [PY20]

Name: HRP Anti-Phosphotyrosine 抗体 [PY20] (ab16389) | Abcam
Brand: Abcam Limited
SKU: AB16389
Price: 77000 JPY
Availability: InStock
Rating: 5 (1 reviews)

HRP Anti-Phosphotyrosine antibody [PY20]

(1 Review)

(19 Publications)

Mouse Monoclonal Phosphotyrosine antibody - conjugated to HRP. Suitable for WB and reacts with Modified Amino Acid samples. Cited in 19 publications.

別名を表示する

L-Phosphotyrosine, L-Tyrosine-O-phosphate, O-Phospho-L-tyrosine, O-Phosphotyrosine, Phospho-L-tyrosine, Phosphonotyrosine

2 Images

Unknown

Western blot - HRP Anti-Phosphotyrosine antibody [PY20] (AB16389)

All lanes:

Western blot - HRP Anti-Phosphotyrosine antibody [PY20] (ab16389) at 1/5000 dilution

All lanes:

NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate at 10 µg

Observed band size: 40 kDa

true

Exposure time: 10s

Unknown

Western blot - HRP Anti-Phosphotyrosine antibody [PY20] (AB16389)

All lanes:

Western blot - HRP Anti-Phosphotyrosine antibody [PY20] (ab16389) at 1/5000 dilution

All lanes:

NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate at 10 µg

Observed band size: 40 kDa

true

Exposure time: 2min

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

PY20

アイソタイプ

IgG2b

標識

HRP

励起波長/蛍光波長

キャリアフリー

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Amino Acid": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Purified native protein": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>" } } }

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製品の詳細

This antibody is known to be inhibited by divalent cations (>1mM) and high salt concentrations (>0.2M).

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Avoid freeze / thaw cycle|Store in the dark

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Phosphotyrosine also known simply as pY is a post-translational modification where a phosphate group binds to the hydroxyl group of a tyrosine amino acid in proteins. This small modification can have a huge impact on proteins' function and interaction. Phosphotyrosine has a molecular weight of approximately 216 Da when it stands on its own. This modification can occur in various proteins expressed widely across tissues playing a role in signaling cell division and morphological changes.

Biological function summary

Phosphorylated tyrosine residues modify the function and activity of proteins within cells. It is a critical component of signal transduction pathways and can alter protein functions when becoming part of large protein complexes. It often regulates receptor proteins and intracellular kinases modifying their ability to interact with other proteins like SH2 and PTB domain-containing proteins. Phosphotyrosine antibodies like anti-phosphotyrosine Ig2 are helpful research tools for detecting these modifications.

Pathways

The modification of tyrosine to phosphotyrosine participates significantly in pathways such as the MAPK/ERK pathway and the PI3K/AKT pathway. These pathways are important for various cellular processes including growth and survival. Proteins like EGFR and PDGFR regulate signaling cascades by phosphorylation of tyrosine residues and often interact with phosphotyrosine and phosphotyrosine antibodies importantly.

The dysregulation of phosphotyrosine levels contributes to conditions like cancer and diabetes. Aberrant activation or overexpression of phosphotyrosine residues on receptors like HER2 and insulin receptors can promote uncontrolled cell proliferation often seen in tumors and contribute to insulin resistance respectively. As such phosphotyrosine serves not only as a biological target but also as a potential therapeutic target in the treatment of these disorders.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information Phosphotyrosine

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文献 (19)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:2342 PubMed37095176

2023

TMEM25 inhibits monomeric EGFR-mediated STAT3 activation in basal state to suppress triple-negative breast cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Bi,Zhihui Wu,Xin Zhang,Taoling Zeng,Wanjun Dai,Ningyuan Qiu,Mingfeng Xu,Yikai Qiao,Lang Ke,Jiayi Zhao,Xinyu Cao,Qi Lin,Xiao Lei Chen,Liping Xie,Zhong Ouyang,Jujiang Guo,Liangkai Zheng,Chao Ma,Shiying Guo,Kangmei Chen,Wei Mo,Guo Fu,Tong-Jin Zhao,Hong-Rui Wang

International journal of environmental research and public health 19: PubMed35010320

2021

Novaluron Has Detrimental Effects on Sperm Functions.

Applications

Unspecified application

Species

Unspecified reactive species

Ju-Mi Hwang,Jeong-Won Bae,Eun-Ju Jung,Woo-Jin Lee,Woo-Sung Kwon

Frontiers in cell and developmental biology 9:660165 PubMed34249914

2021

Desmosterol Incorporation Into Ram Sperm Membrane Before Cryopreservation Improves and Fertility.

Applications

Unspecified application

Species

Unspecified reactive species

María de Las Mercedes Carro,Rafael R A Ramírez-Vasquez,Daniel A Peñalva,Jorgelina Buschiazzo,Federico A Hozbor

Reproductive toxicology (Elmsford, N.Y.) 96:195-201 PubMed32659260

2020

Vanadium adversely affects sperm motility and capacitation status via protein kinase A activity and tyrosine phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jeong-Won Bae,Hobin Im,Ju-Mi Hwang,So-Hye Kim,Lei Ma,Hong Ju Kwon,Eungyung Kim,Myoung Ok Kim,Woo-Sung Kwon

Journal of cell science 133: PubMed31964705

2020

Macrophages show higher levels of engulfment after disruption of interactions between CD47 and the checkpoint receptor SIRPα.

Applications

Unspecified application

Species

Unspecified reactive species

Brandon H Hayes,Richard K Tsai,Lawrence J Dooling,Siddhant Kadu,Justine Y Lee,Diego Pantano,Pia L Rodriguez,Shyamsundar Subramanian,Jae-Won Shin,Dennis E Discher

JACC. Basic to translational science 5:126-144 PubMed32140621

2020

Genetic Deletion of Socs3 in Smooth Muscle Cells Ameliorates Aortic Dissection in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Saki Hirakata,Hiroki Aoki,Satoko Ohno-Urabe,Michihide Nishihara,Aya Furusho,Norifumi Nishida,Sohei Ito,Makiko Hayashi,Hideo Yasukawa,Tsutomu Imaizumi,Sinichi Hiromatsu,Hiroyuki Tanaka,Yoshihiro Fukumoto

The Journal of biological chemistry 294:14953-14965 PubMed31416834

2019

A head-to-toe dimerization has physiological relevance for ligand-induced inactivation of protein tyrosine receptor type Z.

Applications

Unspecified application

Species

Unspecified reactive species

Akihiro Fujikawa,Hajime Sugawara,Naomi Tanga,Kentaro Ishii,Kazuya Kuboyama,Susumu Uchiyama,Ryoko Suzuki,Masaharu Noda

Scientific reports 9:10584 PubMed31332285

2019

Effect of antioxidants on BPA-induced stress on sperm function in a mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Md Saidur Rahman,Kyu-Ho Kang,Sarder Arifuzzaman,Won-Ki Pang,Do-Yeal Ryu,Won-Hee Song,Yoo-Jin Park,Myung-Geol Pang

The Journal of cell biology 218:1725-1742 PubMed30948425

2019

VE-PTP stabilizes VE-cadherin junctions and the endothelial barrier via a phosphatase-independent mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Vanessa V Juettner,Kevin Kruse,Arkaprava Dan,Vinh H Vu,Yousaf Khan,Jonathan Le,Deborah Leckband,Yulia Komarova,Asrar B Malik

Glia 67:967-984 PubMed30667096

2019

The PTN-PTPRZ signal activates the AFAP1L2-dependent PI3K-AKT pathway for oligodendrocyte differentiation: Targeted inactivation of PTPRZ activity in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Naomi Tanga,Kazuya Kuboyama,Ayako Kishimoto,Hiroshi Kiyonari,Aki Shiraishi,Ryoko Suzuki,Toshio Watanabe,Akihiro Fujikawa,Masaharu Noda

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-Phosphotyrosine 抗体 [PY20]

HRP Anti-Phosphotyrosine antibody [PY20]

Western blot - HRP Anti-Phosphotyrosine antibody [PY20] (AB16389)

Western blot - HRP Anti-Phosphotyrosine antibody [PY20] (AB16389)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (19)

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組合せ製品

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Secondary Antibodies

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Abcam product promise