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HRP Anti-Phosphotyrosine 抗体 (ab9329)

Reviews (1)Q&A (1)References (1)
Western blot - HRP Anti-Phosphotyrosine antibody (ab9329)

    Key features and details

    • HRP Rabbit polyclonal to Phosphotyrosine
    • Suitable for: WB, IP, ELISA
    • Reacts with: Species independent
    • Conjugation: HRP
    • Isotype: IgG

    製品の概要

    • 製品名

      HRP Anti-Phosphotyrosine antibody
      Phosphotyrosine 一次抗体 製品一覧

    • 製品の詳細

      HRP Rabbit polyclonal to Phosphotyrosine

    • 由来種

      Rabbit

    • 標識

      HRP

    • アプリケーション

      適用あり: WB, IP, ELISAmore details

    • 種交差性

      交差種: Species independent

    • 免疫原

      Chemical/ Small Molecule corresponding to Phosphotyrosine conjugated to keyhole limpet haemocyanin.

    • ポジティブ･コントロール

      • Use Vanadium treated cell culture (30 min) for Western Blotting and Tyrosine-phosphorylated peptide for ELISA.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab9329の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB (1)
    Use at an assay dependent concentration.
    IP
    Use at an assay dependent concentration.
    ELISA
    Use at an assay dependent concentration.
    特記事項
    WB
    Use at an assay dependent concentration.
    IP
    Use at an assay dependent concentration.
    ELISA
    Use at an assay dependent concentration.

    ターゲット情報

    • 関連性

      The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

    画像

    • Western blot - HRP Anti-Phosphotyrosine antibody (ab9329)
      Western blot - HRP Anti-Phosphotyrosine antibody (ab9329)

      Immunoblotting of fetal mouse brain extract (125 ug - A and 25 ug - B)

      Immunoblotting of fetal mouse brain extract (125 ug - A and 25 ug - B)

    参考文献 (1)

    ab9329 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9329 は 1 報の論文で使用されています。

    • McLaughlin NJ  et al. Platelet-Activating Factor-Mediated Endosome Formation Causes Membrane Translocation of p67phox and p40phox That Requires Recruitment and Activation of p38 MAPK, Rab5a, and Phosphatidylinositol 3-Kinase in Human Neutrophils. J Immunol 180:8192-203 (2008). WB . PubMed: 18523285

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-Phosphotyrosine antibody (HRP)

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (CD4+ murine splenoyctes)
    Loading amount
    50000 cells
    Specification
    CD4+ murine splenoyctes
    Treatment
    anti-CD3 and anti-CD28-coated beads
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Nov 27 2006

    Question

    Im looking to purchase https://www.abcam.com/Phosphotyrosine-antibody-HRP-ab9329.html, do you know the affinity of this antibody for phosphotyrosine?

    Read More
    Answer

    Thank you for contacting us.

    We do not have affinity data for this antibody. If you need an HRP-conjugated antibody, I recommend the mouse monoclonal ab16389:

    Click here (or use the following: https://www.abcam.com/HRP-Phosphotyrosine-antibody-PY20-ab16389.html).

    More data and references for this clone are available on the datasheet for the unconjugated version, ab10321:

    Click here (or use the following: https://www.abcam.com/Phosphotyrosine-antibody-PY20-ab10321.html).

    Please contact us if you have other questions.

    Read More

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