Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

HRP Anti-Myc tag 抗体 [9E10] (ab62928)

Reviews (2)Q&A (1)References (31)
Western blot - HRP Anti-Myc tag antibody [9E10] (ab62928)
  • Western blot - HRP Anti-Myc tag antibody [9E10] (ab62928)

Key features and details

  • HRP Mouse monoclonal [9E10] to Myc tag
  • Suitable for: ELISA, WB
  • Reacts with: Species independent
  • Conjugation: HRP
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 APC Biotin Biotin DyLight® 488 DyLight® 550 DyLight® 650 FITC PE SureLight® APC Unconjugated

製品の概要

  • 製品名

    HRP Anti-Myc tag antibody [9E10]
    Myc tag 一次抗体 製品一覧

  • 製品の詳細

    HRP Mouse monoclonal [9E10] to Myc tag

  • 由来種

    Mouse

  • 標識

    HRP

  • アプリケーション

    適用あり: ELISA, WBmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    Synthetic peptide corresponding to Human Myc tag aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.

  • ポジティブ･コントロール

    • WB: E.coli Whole Cell Lysate (positive control) expressing 11 different epitope tags (ab5395) and Recombinant E.coli Multi Tag protein (ab36791).

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab62928の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
1/100 - 1/500.
WB (2)
1/10000 - 1/50000. Detects a band of approximately 41,45 kDa (predicted molecular weight: 49 kDa).
特記事項
ELISA
1/100 - 1/500.
WB
1/10000 - 1/50000. Detects a band of approximately 41,45 kDa (predicted molecular weight: 49 kDa).

ターゲット情報

  • 関連性

    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

  • 細胞内局在

    Nuclear

  • 別名

    • c-myc tag antibody
    • Myc Epitope Tag antibody

画像

  • Western blot - HRP Anti-Myc tag antibody [9E10] (ab62928)
    Western blot - HRP Anti-Myc tag antibody [9E10] (ab62928)
    HRP Anti-Myc tag antibody [9E10] (ab62928) at 1/10000 dilution + Recombinant E. coli Multi Tag protein (ab36791) at 0.5 µg

    Predicted band size: 49 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?


    Exposure time: 4 minutes


    Blocking buffer: 2% BSA
    Gel type: MOPS

  • Western blot - HRP Anti-Myc tag antibody [9E10] (ab62928)
    Western blot - HRP Anti-Myc tag antibody [9E10] (ab62928)
    HRP Anti-Myc tag antibody [9E10] (ab62928) at 1/10000 dilution + E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.1 µg

    Performed under reducing conditions.

    Predicted band size: 49 kDa
    Observed band size: 41 kDa why is the actual band size different from the predicted?

参考文献 (31)

ab62928 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab62928 は 31 報の論文で使用されています。

  • Lu Y  et al. A Novel Vpu Adaptive Mutation of HIV-1 Degrades Tetherin in Northern Pig-Tailed Macaques (Macaca leonina) Mainly via the Ubiquitin-Proteasome Pathway and Increases Viral Release. J Virol 97:e0020023 (2023). PubMed: 36971578
  • Daoud M  et al. XIAP promotes melanoma growth by inducing tumour neutrophil infiltration. EMBO Rep 23:e53608 (2022). PubMed: 35437868
  • He W  et al. Targeted demethylation at ZNF154 promotor upregulates ZNF154 expression and inhibits the proliferation and migration of Esophageal Squamous Carcinoma cells. Oncogene 41:4537-4546 (2022). PubMed: 36064578
  • Jin JO  et al. Sequential ubiquitination of p53 by TRIM28, RLIM, and MDM2 in lung tumorigenesis. Cell Death Differ 28:1790-1803 (2021). PubMed: 33328571
  • Shailesh H  et al. Protein arginine methyltransferase 5 (PRMT5) activates WNT/ß-catenin signalling in breast cancer cells via epigenetic silencing of DKK1 and DKK3. J Cell Mol Med 25:1583-1600 (2021). PubMed: 33462997
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-3 of 3 Abreviews or Q&A

Western blot abreview for Anti-c-Myc antibody [9E10] (HRP)

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Escherichia coli Recombinant protein (BL21(DE3))
Loading amount
0.5 µg
Specification
BL21(DE3)
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 30 2010

Western blot abreview for Anti-c-Myc antibody [9E10] (HRP)

 Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (293T cells)
Loading amount
20 µg
Specification
293T cells
Gel Running Conditions
Reduced Denaturing (Invitrogen NUPAGE Bis-Tris Gel 4-12% Gradient Gel MES Buffer)
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 25 2010

Question

Do you have information on the sensitivity of your anti-Myc-tag antibody? What is the minimal amount of protein I need to load onto a gel to detect my Myc-tagged fusion protein?

Read More
Answer

The laboratory confirmed that they tested 0.1ug E. coli with myc-tag in WB and they obtained a good signal at 1 min exposure. However, they did not test the limits of ab62928 products sensitivity, but they would expect it to be able to detect smaller quantities than they have used.


Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com