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リコンビナントRabMAb

HRP Anti-Maltose Binding Protein 抗体 [EPR4744] (ab300054)

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Western blot - HRP Anti-Maltose Binding Protein antibody [EPR4744] (AB300054)
  • ELISA - HRP Anti-Maltose Binding Protein antibody [EPR4744] (AB300054)
  • HRP Anti-Maltose Binding Protein antibody [EPR4744] (AB300054)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR4744] to Maltose Binding Protein
  • Suitable for: ELISA, WB
  • Reacts with: Escherichia coli
  • Conjugation: HRP

Conjugates logo Related conjugates and formulations

Carrier Free Unconjugated

製品の概要

  • 製品名

    HRP Anti-Maltose Binding Protein antibody [EPR4744]
    Maltose Binding Protein 一次抗体 製品一覧

  • 製品の詳細

    HRP Rabbit monoclonal [EPR4744] to Maltose Binding Protein

  • 由来種

    Rabbit

  • 標識

    HRP

  • アプリケーション

    適用あり: ELISA, WBmore details

  • 種交差性

    交差種: Escherichia coli

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: E.coli lysate

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.

  • バッファー

    pH: 7.4
    Preservative: 0.1% Proclin 300 Solution
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR4744

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300054の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
Use a concentration of 0.5 µg/ml.

Primary antibody concentration range: 4000-0 ng/ml
WB
1/5000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
特記事項
ELISA
Use a concentration of 0.5 µg/ml.

Primary antibody concentration range: 4000-0 ng/ml
WB
1/5000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

ターゲット情報

  • 関連性

    Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Cleavage by factor Xa separates MBP from its partner protein.

  • 細胞内局在

    Periplasm

  • 参照データベース

    • 別名

      • ECK4026 antibody
      • JW3994 antibody
      • Mal E antibody
      • MalE antibody
      • malJ antibody
      • Maltodextrin binding protein antibody
      • Maltose ABC transporter periplasmic protein antibody
      • Maltose binding periplasmic protein antibody
      • MMBP antibody
      • Periplasmic maltose binding protein antibody
      see all

    画像

    • Western blot - HRP Anti-Maltose Binding Protein antibody [EPR4744] (AB300054)
      Western blot - HRP Anti-Maltose Binding Protein antibody [EPR4744] (AB300054)
      HRP Anti-Maltose Binding Protein antibody [EPR4744] (ab300054) at 1/5000 dilution + E.coli lysate at 20 µg

      Predicted band size: 43 kDa
      Observed band size: 43 kDa


      Exposure time: 3 minutes


      Blocking / Diluting buffer and concentration:

      5% NFDM/TBST

    • ELISA - HRP Anti-Maltose Binding Protein antibody [EPR4744] (AB300054)
      ELISA - HRP Anti-Maltose Binding Protein antibody [EPR4744] (AB300054)

      Direct ELISA Dose Response Curve for Maltose Binding Protein using ab300054:

      Antigen: Maltose binding protein at 1000 ng/ml

      Primary antibody concentration range: 4000-0 ng/ml

    • HRP Anti-Maltose Binding Protein antibody [EPR4744] (AB300054)
      HRP Anti-Maltose Binding Protein antibody [EPR4744] (AB300054)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300054 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300054 は論文での使用が確認できていません。

    レビューと Q&A

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    There are currently no Customer reviews or Questions for ab300054.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com