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HRP Anti-HIV1 p24 抗体 (ab20365)

Reviews (1)Q&A (1)References (3)

Key features and details

  • HRP Goat polyclonal to HIV1 p24
  • Suitable for: ELISA, ICC, WB
  • Reacts with: Human immunodeficiency virus
  • Conjugation: HRP
  • Isotype: IgG

製品の概要

  • 製品名

    HRP Anti-HIV1 p24 antibody
    HIV1 p24 一次抗体 製品一覧

  • 製品の詳細

    HRP Goat polyclonal to HIV1 p24

  • 由来種

    Goat

  • 標識

    HRP

  • 特異性

    HIV1 p24 antibody (ab20365) targets p24 (predominate p24 band pattern in Western blot). Does not cross-react with human T or B cells. Antiserum is not reactive with human or bovine sera.

  • アプリケーション

    適用あり: ELISA, ICC, WBmore details

  • 種交差性

    交差種: Human immunodeficiency virus

  • 免疫原

    Full length native protein (purified native p24 from strain IIIb).

  • 特記事項

    Purified IgG fraction covalently coupled to a highly purified preparation of horseradish peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is removed.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab20365の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA (1)
1/200 - 1/1000.
ICC
1/20 - 1/200.
WB
1/200 - 1/1000. Predicted molecular weight: 56 kDa. Dilution optimised using Chromogenic detection. When using chemiluminescent substrates, the assay will require much less antibody. Depending on the sensitivity of the substrate, the antibody dilution may be 1/10,000 - 1/100,000.
特記事項
ELISA
1/200 - 1/1000.
ICC
1/20 - 1/200.
WB
1/200 - 1/1000. Predicted molecular weight: 56 kDa. Dilution optimised using Chromogenic detection. When using chemiluminescent substrates, the assay will require much less antibody. Depending on the sensitivity of the substrate, the antibody dilution may be 1/10,000 - 1/100,000.

ターゲット情報

  • 関連性

    HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.

  • 細胞内局在

    Membrane

  • 参照データベース

    • 別名

      • CA antibody
      • Capsid protein p24 antibody
      • HIV-1 Gag p24 antibody
      • HIV1gp1 antibody
      • Human immunodeficiency virus 1 antibody
      • Human immunodeficiency virus type 1 p24 antibody
      see all

    参考文献 (3)

    ab20365 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab20365 は 3 報の論文で使用されています。

    • Tan Z  et al. Eliminating mesothelioma by AAV-vectored, PD1-based vaccination in the tumor microenvironment. Mol Ther Oncolytics 20:373-386 (2021). PubMed: 33614918
    • Peyret H  et al. Improving plant transient expression through the rational design of synthetic 5' and 3' untranslated regions. Plant Methods 15:108 (2019). PubMed: 31548848
    • Guth CA & Sodroski J Contribution of PDZD8 to stabilization of the human immunodeficiency virus type 1 capsid. J Virol 88:4612-23 (2014). ELISA . PubMed: 24554657

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    ELISA abreview for Anti-HIV1 p24 antibody (HRP)

     Excellent
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    HIV Purified protein (purified from bacteria overexpression)
    Specification
    purified from bacteria overexpression
    Type
    Direct
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Shijian Zhang

    Verified customer

    投稿 Jun 03 2014

    Question

    I'm doing an ELISPOT-type assay with these products and am looking for a protocol.

    Read More
    Answer

    Thank you for your inquiry. I know that you said you're using nitrocellulose, and this protocol uses PVDF, but I hope you can adjust it accordingly. https://www.abcam.com/index.html?pageconfig=resource&rid=11456 I hope this information helps. Please contact us with any other questions.

    Read More

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