HRP Anti-HIV1 p24 抗体 (ab20365)
Key features and details
- HRP Goat polyclonal to HIV1 p24
- Suitable for: ELISA, ICC, WB
- Reacts with: Human immunodeficiency virus
- Conjugation: HRP
- Isotype: IgG
製品の概要
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製品名
HRP Anti-HIV1 p24 antibody
HIV1 p24 一次抗体 製品一覧 -
製品の詳細
HRP Goat polyclonal to HIV1 p24 -
由来種
Goat -
標識
HRP -
特異性
HIV1 p24 antibody (ab20365) targets p24 (predominate p24 band pattern in Western blot). Does not cross-react with human T or B cells. Antiserum is not reactive with human or bovine sera. -
アプリケーション
適用あり: ELISA, ICC, WBmore details -
種交差性
交差種: Human immunodeficiency virus -
免疫原
Full length native protein (purified native p24 from strain IIIb).
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特記事項
Purified IgG fraction covalently coupled to a highly purified preparation of horseradish peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is removed.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Preservative: 0.002% Thimerosal (merthiolate)
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
IgG fraction -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Recombinant Protein
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アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab20365の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ELISA | (1) |
1/200 - 1/1000.
|
ICC |
1/20 - 1/200.
|
|
WB |
1/200 - 1/1000. Predicted molecular weight: 56 kDa. Dilution optimised using Chromogenic detection. When using chemiluminescent substrates, the assay will require much less antibody. Depending on the sensitivity of the substrate, the antibody dilution may be 1/10,000 - 1/100,000.
|
特記事項 |
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ELISA
1/200 - 1/1000. |
ICC
1/20 - 1/200. |
WB
1/200 - 1/1000. Predicted molecular weight: 56 kDa. Dilution optimised using Chromogenic detection. When using chemiluminescent substrates, the assay will require much less antibody. Depending on the sensitivity of the substrate, the antibody dilution may be 1/10,000 - 1/100,000. |
ターゲット情報
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関連性
HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex. -
細胞内局在
Membrane -
参照データベース
- SwissProt: P04585 Human immunodeficiency virus
- SwissProt: P12497 Human immunodeficiency virus
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別名
- CA antibody
- Capsid protein p24 antibody
- HIV-1 Gag p24 antibody
see all
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab20365 は 3 報の論文で使用されています。
- Tan Z et al. Eliminating mesothelioma by AAV-vectored, PD1-based vaccination in the tumor microenvironment. Mol Ther Oncolytics 20:373-386 (2021). PubMed: 33614918
- Peyret H et al. Improving plant transient expression through the rational design of synthetic 5' and 3' untranslated regions. Plant Methods 15:108 (2019). PubMed: 31548848
- Guth CA & Sodroski J Contribution of PDZD8 to stabilization of the human immunodeficiency virus type 1 capsid. J Virol 88:4612-23 (2014). ELISA . PubMed: 24554657