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AB190584

HRP Anti-GFP 抗体 [E385]

HRP Anti-GFP antibody [E385]

5

(1 Review)

|

(9 Publications)

Rabbit Recombinant Monoclonal GFP antibody - conjugated to HRP. Suitable for WB. Cited in 9 publications.

See more GFP antibodies and assay kits

別名を表示する

Green fluorescent protein, GFP

2 Images
Western blot - HRP Anti-GFP antibody [E385] (AB190584)
  • WB

Lab

Western blot - HRP Anti-GFP antibody [E385] (AB190584)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab190485 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-GFP antibody [E385] (ab190584) at 1/10000 dilution

Lane 1:

HEK 293 over-expressing GFP at 5 µg

Lane 2:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 5 µg

Predicted band size: 27 kDa

Observed band size: 27 kDa

true

Exposure time: 30s

Western blot - HRP Anti-GFP antibody [E385] (AB190584)
  • WB

Lab

Western blot - HRP Anti-GFP antibody [E385] (AB190584)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab190584 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-GFP antibody [E385] (ab190584) at 1/10000 dilution

Lane 1:

Western blot - Recombinant A. victoria GFP protein (<a href='/products/proteins-peptides/recombinant-a-victoria-gfp-protein-ab84191'>ab84191</a>) at 0.1 µg

Lane 2:

Western blot - Recombinant RFP protein (<a href='/products/proteins-peptides/recombinant-rfp-protein-ab51993'>ab51993</a>) at 0.1 µg

Predicted band size: 27 kDa

Observed band size: 27 kDa

true

Exposure time: 10s

関連する標識済み抗体及び組成の異なる製品 (2)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

E385

アイソタイプ

IgG

標識

HRP

励起波長/蛍光波長
キャリアフリー

No

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
See full target information GFP
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文献 (9)

Recent publications for all applications. Explore the full list and refine your search

The New phytologist 240:1467-1483 PubMed37658678

2023

Plant immunity suppressor SKRP encodes a novel RNA-binding protein that targets exon 3' end of unspliced RNA.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Chen,Zhihui Xu,Jie Huang,Haidong Shu,Yufan Hui,Danling Zhu,Yufeng Wu,Suomeng Dong,Zhe Wu

eLife 10: PubMed34397383

2021

A CTP-dependent gating mechanism enables ParB spreading on DNA.

Applications

Unspecified application

Species

Unspecified reactive species

Adam Sb Jalal,Ngat T Tran,Clare Em Stevenson,Afroze Chimthanawala,Anjana Badrinarayanan,David M Lawson,Tung Bk Le

Science advances 7: PubMed33523899

2021

Overlap of NatA and IAP substrates implicates N-terminal acetylation in protein stabilization.

Applications

Unspecified application

Species

Unspecified reactive species

Franziska Mueller,Alexandra Friese,Claudio Pathe,Richard Cardoso da Silva,Kenny Bravo Rodriguez,Andrea Musacchio,Tanja Bange

Open biology 10:200343 PubMed33352067

2020

Novel perspectives of target-binding by the evolutionarily conserved PP4 phosphatase.

Applications

Unspecified application

Species

Unspecified reactive species

Zoltan Karman,Zsuzsanna Rethi-Nagy,Edit Abraham,Lilla Fabri-Ordogh,Akos Csonka,Peter Vilmos,Janusz Debski,Michal Dadlez,David M Glover,Zoltan Lipinszki

Molecular cell 81:67-87.e9 PubMed33248027

2020

BUB1 and CENP-U, Primed by CDK1, Are the Main PLK1 Kinetochore Receptors in Mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Priyanka Singh,Marion E Pesenti,Stefano Maffini,Sara Carmignani,Marius Hedtfeld,Arsen Petrovic,Anupallavi Srinivasamani,Tanja Bange,Andrea Musacchio

Scientific reports 10:17095 PubMed33051543

2020

Covalent protein display on Hepatitis B core-like particles in plants through the in vivo use of the SpyTag/SpyCatcher system.

Applications

Unspecified application

Species

Unspecified reactive species

Hadrien Peyret,Daniel Ponndorf,Yulia Meshcheriakova,Jake Richardson,George P Lomonossoff

Plant signaling & behavior 15:1791519 PubMed32657215

2020

Characterization of GFP-AtPEN1 as a marker protein for extracellular vesicles isolated from leaves.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaojiao Zhang,Yanglin Qiu,Kai Xu

BMC microbiology 18:5 PubMed29433439

2018

Legionella effector AnkX interacts with host nuclear protein PLEKHN1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaobo Yu,Rebecca R Noll,Barbara P Romero Dueñas,Samual C Allgood,Kristi Barker,Jeffrey L Caplan,Matthias P Machner,Joshua LaBaer,Ji Qiu,M Ramona Neunuebel

Nature methods 14:629-635 PubMed28417999

2017

A tiling-deletion-based genetic screen for cis-regulatory element identification in mammalian cells.

Applications

WB

Species

Unspecified reactive species

Yarui Diao,Rongxin Fang,Bin Li,Zhipeng Meng,Juntao Yu,Yunjiang Qiu,Kimberly C Lin,Hui Huang,Tristin Liu,Ryan J Marina,Inkyung Jung,Yin Shen,Kun-Liang Guan,Bing Ren
View all publications
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