JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB190584

HRP Anti-GFP 抗体 [E385]

HRP Anti-GFP antibody [E385]

5

(1 Review)

|

(16 Publications)

Rabbit Recombinant Monoclonal GFP antibody - conjugated to HRP. Suitable for WB. Cited in 9 publications.

See more GFP antibodies and assay kits

別名を表示する

Green fluorescent protein, GFP

2 Images
Western blot - HRP Anti-GFP antibody [E385] (AB190584)
  • WB

Lab

Western blot - HRP Anti-GFP antibody [E385] (AB190584)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab190584 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-GFP antibody [E385] (ab190584) at 1/10000 dilution

Lane 1:

Western blot - Recombinant A. victoria GFP protein (Tag Free) (<a href='/products/proteins-peptides/recombinant-a-victoria-gfp-protein-ab84191'>ab84191</a>) at 0.1 µg

Lane 2:

Western blot - Recombinant RFP protein (<a href='/products/proteins-peptides/recombinant-rfp-protein-ab51993'>ab51993</a>) at 0.1 µg

Predicted band size: 27 kDa

Observed band size: 27 kDa

true

Exposure time: 10s

Western blot - HRP Anti-GFP antibody [E385] (AB190584)
  • WB

Lab

Western blot - HRP Anti-GFP antibody [E385] (AB190584)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab190485 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-GFP antibody [E385] (ab190584) at 1/10000 dilution

Lane 1:

HEK 293 over-expressing GFP at 5 µg

Lane 2:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 5 µg

Predicted band size: 27 kDa

Observed band size: 27 kDa

true

Exposure time: 30s

関連する標識済み抗体及び組成の異なる製品 (2)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

E385

アイソタイプ

IgG

標識

HRP

励起波長/蛍光波長
キャリアフリー

No

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

代替製品

Primary Antibodies

AB290

Anti-GFP antibody

Immunohistochemistry - Free Floating - Anti-GFP antibody (AB290)

  • 5
  • 180
Primary Antibodies

AB291

Anti-GFP antibody [LGB-1]

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [LGB-1] (AB291)

  • 4
  • 6
Primary Antibodies

AB5449

Anti-GFP antibody

Lab Essentials

Western blot - Anti-GFP antibody (AB5449)

  • 4
  • 1
Primary Antibodies

AB6556

Anti-GFP antibody

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (AB6556)

  • 5
  • 61
Primary Antibodies

AB5450

Anti-GFP antibody

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (AB5450)

  • 5
  • 13
Primary Antibodies

AB69314

Sepharose Anti-GFP antibody

Immunoprecipitation - Sepharose Anti-GFP antibody (AB69314)

  • 4
  • 1
Primary Antibodies

AB6661

Alkaline Phosphatase Anti-GFP antibody

Western blot - Alkaline Phosphatase Anti-GFP antibody (AB6661)

  • 0
  • 0
Primary Antibodies

AB6662

FITC Anti-GFP antibody

Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (AB6662)

  • 4
  • 15
Primary Antibodies

AB6663

HRP Anti-GFP antibody

Western blot - HRP Anti-GFP antibody (AB6663)

  • 0
  • 0
Primary Antibodies

AB1218

Anti-GFP antibody [9F9.F9]

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [9F9.F9] (AB1218)

  • 4
  • 44
Primary Antibodies

AB6658

Biotin Anti-GFP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-GFP antibody (AB6658)

  • 5
  • 6
Primary Antibodies

AB6673

Anti-GFP antibody

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody (AB6673)

  • 4
  • 26
Primary Antibodies

AB92456

Anti-GFP antibody

  • 0
  • 0
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB183735

Anti-GFP antibody [EPR14104-89]

20ul selling size|RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody [EPR14104-89] (AB183735)

  • 0
  • 0

組合せ製品

Assay Kits

AB235672

GFP Quantification Kit

Functional Studies - GFP Quantification Kit (AB235672)

  • 0
  • 0
Primary Antibodies

AB167453

Anti-mCherry antibody

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody (AB167453)

  • 5
  • 17
Primary Antibodies

AB34771

Biotin Anti-RFP antibody

Western blot - Biotin Anti-RFP antibody (AB34771)

  • 4
  • 5
Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

  • 5
  • 8
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>" } } }
style
left-column

製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
style
left-column
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
See full target information GFP
style
left-column

文献 (16)

Recent publications for all applications. Explore the full list and refine your search

Journal of lipid research 65:100663 PubMed39369791

2024

A fatty acid-ordered plasma membrane environment is critical for Ebola virus matrix protein assembly and budding.

Applications

Unspecified application

Species

Unspecified reactive species

Souad Amiar,Kristen A Johnson,Monica L Husby,Andrea Marzi,Robert V Stahelin

Nature communications 15:4322 PubMed38773107

2024

The SUN-family protein Sad1 mediates heterochromatin spatial organization through interaction with histone H2A-H2B.

Applications

Unspecified application

Species

Unspecified reactive species

Wenqi Sun,Qianhua Dong,Xueqing Li,Jinxin Gao,Xianwen Ye,Chunyi Hu,Fei Li,Yong Chen

Nature communications 15:2399 PubMed38493158

2024

The spliceosome-associated protein CWC15 promotes miRNA biogenesis in Arabidopsis.

Applications

Unspecified application

Species

Unspecified reactive species

Bangjun Zhou,Huihui Yu,Yong Xue,Mu Li,Chi Zhang,Bin Yu

Science advances 9:eadi9036 PubMed37967186

2023

ACD15, ACD21, and SLN regulate the accumulation and mobility of MBD6 to silence genes and transposable elements.

Applications

Unspecified application

Species

Unspecified reactive species

Brandon A Boone,Lucia Ichino,Shuya Wang,Jason Gardiner,Jaewon Yun,Yasaman Jami-Alahmadi,Jihui Sha,Cristy P Mendoza,Bailey J Steelman,Aliya van Aardenne,Sophia Kira-Lucas,Isabelle Trentchev,James A Wohlschlegel,Steven E Jacobsen

The New phytologist 240:1467-1483 PubMed37658678

2023

Plant immunity suppressor SKRP encodes a novel RNA-binding protein that targets exon 3' end of unspliced RNA.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Chen,Zhihui Xu,Jie Huang,Haidong Shu,Yufan Hui,Danling Zhu,Yufeng Wu,Suomeng Dong,Zhe Wu

ACS chemical biology 18:583-594 PubMed36795767

2023

Screen for Modulation of Nucleocapsid Protein Condensation Identifies Small Molecules with Anti-Coronavirus Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Tong Quek,Kierra S Hardy,Stephen G Walker,Dan T Nguyen,Taciani de Almeida Magalhães,Adrian Salic,Sujatha M Gopalakrishnan,Pamela A Silver,Timothy J Mitchison

Biochemical and biophysical research communications 639:54-61 PubMed36470072

2022

Ser815 Phosphorylation stabilizes the androgen receptor homodimer and stimulates ER-stress induced cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Kosuke Yokobori,Masahiko Negishi

Plant biotechnology journal 20:1363-1372 PubMed35325498

2022

Plant-based expression and characterization of SARS-CoV-2 virus-like particles presenting a native spike protein.

Applications

Unspecified application

Species

Unspecified reactive species

Jae-Wan Jung,Gergana Zahmanova,Ivan Minkov,George P Lomonossoff

eLife 10: PubMed34397383

2021

A CTP-dependent gating mechanism enables ParB spreading on DNA.

Applications

Unspecified application

Species

Unspecified reactive species

Adam Sb Jalal,Ngat T Tran,Clare Em Stevenson,Afroze Chimthanawala,Anjana Badrinarayanan,David M Lawson,Tung Bk Le

Science advances 7: PubMed33523899

2021

Overlap of NatA and IAP substrates implicates N-terminal acetylation in protein stabilization.

Applications

Unspecified application

Species

Unspecified reactive species

Franziska Mueller,Alexandra Friese,Claudio Pathe,Richard Cardoso da Silva,Kenny Bravo Rodriguez,Andrea Musacchio,Tanja Bange
View all publications
style
left-column
style
left-column
style
right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com