HRP Anti-GAPDH 抗体 [mAbcam 9484] - Loading Control
HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control
5
(20 Reviews)
|
(252 Publications)
Anti-GAPDH antibody [mAbcam 9484] - Loading Control - HRP conjugated (ab9482) is a mouse monoclonal antibody detecting GAPDH in Western Blot. Suitable for Human, Mouse, Rat.
- Over 220 publications
- Trusted since 2004
別名を表示する
GAPD, CDABP0047, OK/SW-cl.12, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH
- WB
Lab
Western blot - HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (AB9482)
All lanes:
Western blot - HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482) at 1/1000 dilution
Lane 1:
HeLa Whole Cell Lysate at 10 µg
Lane 2:
Hek293 Whole Cell Lysate at 10 µg
Lane 3:
NIH 3T3 Whole Cel lLysate at 10 µg
Lane 4:
PC12 Whole Cell Lysate at 10 µg
Predicted band size: 36 kDa
Observed band size: 37 kDa
true
Exposure time: 4min
関連する標識済み抗体及び組成の異なる製品 (4)
-
702 Alexa Fluor® 680
Alexa Fluor® 680 Anti-GAPDH antibody [mAbcam 9484] - Loading Control
-
805 Alexa Fluor® 790
Alexa Fluor® 790 Anti-GAPDH antibody [mAbcam 9484] - Loading Control
-
665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-GAPDH antibody [mAbcam 9484]
-
Anti-GAPDH antibody [mAbcam 9484] - Loading Control
Reactivity data
製品の詳細
HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482) is a mouse monoclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rat samples.
What is the molecular weight of GAPDH?
Anti-GAPDH [mAbcam 9484] - Loading Control HRP (ab9482) is often used as a loading control in Western blot. It specifically detects a band for GAPDH (UniProt: P04406) at a molecular weight of 40.2kDa.
Trusted by the scientific community
Anti-GAPDH [mAbcam 9484] - Loading Control HRP (ab9482) was first used in a scientific publication in 2004 and has been cited over 220 times in peer-reviewed journals.
Reviewed by scientists
Anti-GAPDH [mAbcam 9484] - Loading Control HRP (ab9482) has over 15 independent reviews from customers.
Related products
Antibody clone mAbcam 9484 is also available pre-conjugated to a variety of labels for your convenience – Anti-GAPDH HRP [mAbcam 9484] - Loading Control (ab9482).
Other related products
We have a range of other formats of antibody clone [mAbcam 9484] also available for your convenience: ab9484, Alexa Fluor® 680 - ab184095, Alexa Fluor® 790 - ab184578, Alexa Fluor® 647 - ab196697
出荷温度及び保存条件
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補足情報
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.
Pathways
GAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.
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文献 (252)
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