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AB9482

HRP Anti-GAPDH 抗体 [mAbcam 9484] - Loading Control

HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control

5

(20 Reviews)

|

(252 Publications)

Anti-GAPDH antibody [mAbcam 9484] - Loading Control - HRP conjugated (ab9482) is a mouse monoclonal antibody detecting GAPDH in Western Blot. Suitable for Human, Mouse, Rat.

- Over 220 publications
- Trusted since 2004

別名を表示する

GAPD, CDABP0047, OK/SW-cl.12, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH

1 Images
Western blot - HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (AB9482)
  • WB

Lab

Western blot - HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (AB9482)

All lanes:

Western blot - HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482) at 1/1000 dilution

Lane 1:

HeLa Whole Cell Lysate at 10 µg

Lane 2:

Hek293 Whole Cell Lysate at 10 µg

Lane 3:

NIH 3T3 Whole Cel lLysate at 10 µg

Lane 4:

PC12 Whole Cell Lysate at 10 µg

Predicted band size: 36 kDa

Observed band size: 37 kDa

true

Exposure time: 4min

関連する標識済み抗体及び組成の異なる製品 (4)

  • 702 Alexa Fluor® 680

    Alexa Fluor® 680 Anti-GAPDH antibody [mAbcam 9484] - Loading Control

  • 805 Alexa Fluor® 790

    Alexa Fluor® 790 Anti-GAPDH antibody [mAbcam 9484] - Loading Control

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-GAPDH antibody [mAbcam 9484]

  • Unconjugated

    Anti-GAPDH antibody [mAbcam 9484] - Loading Control

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

mAbcam 9484

アイソタイプ

IgG2b

軽鎖のタイプ

kappa

標識

HRP

励起波長/蛍光波長
キャリアフリー

No

交差種

Mouse, Rat, Human

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p>NOT SUITABLE for blocking with milk. Block in 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this ab following concerning customer feedback on the lack of signal with some vials. We found that milk significantly decreases signal and is therefore not a suitable blocking agent for this ab (see images of our tests on the datasheet of the unconjugated version, <a href='/products/primary-antibodies/gapdh-antibody-mabcam-9484-loading-control-ab9484'>ab9484</a>). This change in characteristics of the ab is due to a recent update to the production process.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p>NOT SUITABLE for blocking with milk. Block in 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this ab following concerning customer feedback on the lack of signal with some vials. We found that milk significantly decreases signal and is therefore not a suitable blocking agent for this ab (see images of our tests on the datasheet of the unconjugated version, <a href='/products/primary-antibodies/gapdh-antibody-mabcam-9484-loading-control-ab9484'>ab9484</a>). This change in characteristics of the ab is due to a recent update to the production process.</p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p>NOT SUITABLE for blocking with milk. Block in 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this ab following concerning customer feedback on the lack of signal with some vials. We found that milk significantly decreases signal and is therefore not a suitable blocking agent for this ab (see images of our tests on the datasheet of the unconjugated version, <a href='/products/primary-antibodies/gapdh-antibody-mabcam-9484-loading-control-ab9484'>ab9484</a>). This change in characteristics of the ab is due to a recent update to the production process.</p>" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

製品の詳細

What is this antibody validated in?
HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482) is a mouse monoclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rat samples.

What is the molecular weight of GAPDH?
Anti-GAPDH [mAbcam 9484] - Loading Control HRP (ab9482) is often used as a loading control in Western blot. It specifically detects a band for GAPDH (UniProt: P04406) at a molecular weight of 40.2kDa.

Trusted by the scientific community
Anti-GAPDH [mAbcam 9484] - Loading Control HRP (ab9482) was first used in a scientific publication in 2004 and has been cited over 220 times in peer-reviewed journals.

Reviewed by scientists
Anti-GAPDH [mAbcam 9484] - Loading Control HRP (ab9482) has over 15 independent reviews from customers.

Related products
Antibody clone mAbcam 9484 is also available pre-conjugated to a variety of labels for your convenience – Anti-GAPDH HRP [mAbcam 9484] - Loading Control (ab9482).

Other related products
We have a range of other formats of antibody clone [mAbcam 9484] also available for your convenience: ab9484, Alexa Fluor® 680 - ab184095, Alexa Fluor® 790 - ab184578, Alexa Fluor® 647 - ab196697

出荷温度及び保存条件

製品の状態
Liquid
精製度
IgG fraction
バッファー組成
Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Glyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.
Biological function summary

GAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.

Pathways

GAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.

GAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH’s altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.

製品プロトコール

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ターゲットの情報

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed : 11724794, PubMed : 3170585). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed : 11724794, PubMed : 3170585). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed : 23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed : 23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed : 23332158, PubMed : 27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).
See full target information GAPDH

文献 (252)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113338 PubMed40933646

2025

Functional variants of CFAP410 affect the DNA damage response leading to motor neuron degeneration - Implications for ALS.

Applications

Unspecified application

Species

Unspecified reactive species

Ross Ferguson,Vasanta Subramanian

Frontiers in cell and developmental biology 13:1623135 PubMed40666290

2025

ELK1 regulates BMPR1B transcriptional activity in ovine granulosa cells.

Applications

Unspecified application

Species

Unspecified reactive species

Anwar Abdurahman,Yuling Ga,Xuehai Ma

Discover oncology 16:1099 PubMed40515890

2025

Single-cell transcriptomics identify a chemotherapy-resistance related cluster overexpressed CLIC3 in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhefeng Li,Jie Li,Yue Li,Junjie Yi,Xiaoting Zhao,Wentao Yue

Molecular genetics and genomics : MGG 300:46 PubMed40304806

2025

Identification of oxidative stress-related subgroups and signature genes for the prediction of prognosis and immune microenvironment in thyroid cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenwei Qiu,Jing Li,Mei Tian

European journal of histochemistry : EJH 69: PubMed40272258

2025

Effect of CDX2 on proliferation, invasion, migration, and apoptosis of duodenal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Pan,Yi Zhao,Yu Zhang,Yuhe Zhou,Fengxuan Zhang,Yitian Chen,Xiaoyuan Chu

Nature communications 16:2135 PubMed40032835

2025

Proximity proteomics reveals a mechanism of fatty acid transfer at lipid droplet-mitochondria- endoplasmic reticulum contact sites.

Applications

Unspecified application

Species

Unspecified reactive species

Ayenachew Bezawork-Geleta,Camille J Devereux,Stacey N Keenan,Jieqiong Lou,Ellie Cho,Shuai Nie,David P De Souza,Vinod K Narayana,Nicole A Siddall,Carlos H M Rodrigues,Stephanie Portelli,Tenghao Zheng,Hieu T Nim,Mirana Ramialison,Gary R Hime,Garron T Dodd,Elizabeth Hinde,David B Ascher,David A Stroud,Matthew J Watt

Scientific reports 14:31086 PubMed39730651

2024

A novel biomarker of COVI-19: MMP8 emerged by integrated bulk RNAseq and single-cell sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenguo Liu,Shunda Wang

Communications biology 7:1174 PubMed39294274

2024

CTBP1 links metabolic syndrome to polycystic ovary syndrome through interruption of aromatase and SREBP1.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Kong,Guang Yang,Xu Feng,Zhaodong Ji,Xiaoling Wang,Yang Shao,Jiao Meng,Guidong Yao,Chunxia Ren,Gong Yang

Nature cardiovascular research 3:186-202 PubMed39196188

2024

Cysteine-rich with EGF-like domains 2 (CRELD2) is an endoplasmic reticulum stress-inducible angiogenic growth factor promoting ischemic heart repair.

Applications

Unspecified application

Species

Unspecified reactive species

Xuekun Wu,Linqun Zheng,Marc R Reboll,Lillian F Hyde,Elvira Mass,Hans W Niessen,Maike Kosanke,Andreas Pich,Evangelos Giannitsis,Jochen Tillmanns,Johann Bauersachs,Joerg Heineke,Yong Wang,Mortimer Korf-Klingebiel,Felix Polten,Kai C Wollert

International journal of molecular sciences 25: PubMed38732031

2024

CRISPR Screen Identifies the RNA-Binding Protein Eef1a1 as a Key Regulator of Myogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Liu,Wei Wang,Zishuai Wang,Xinhao Fan,Wangchang Li,Yuxin Huang,Xiaogan Yang,Zhonglin Tang
View all publications

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