HRP Anti-GAPDH 抗体 - Loading Control

• WB

Ap

Western blot - HRP Anti-GAPDH antibody - Loading Control (AB9385)

The membrane 1-4 was blocked in 5% BSA (1 hour). The membrane 5-8 was blocked in 5% MILK (1 hour). Abcam routinely uses 5% BSA to block, however following recent customer feedback our labs investigated the effect of 5% milk blocking. We can now confirm that milk is not a suitable blocking agent for this antibody and significantly decreases the signal on the membrane.

All lanes:

Western blot - HRP Anti-GAPDH antibody - Loading Control (ab9385) at 1/5000 dilution

Lanes 1 and 5:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 10 µg

Lanes 2 and 6:

Jurkat (Human) Whole Cell Lysate (ab52254) at 10 µg

Lanes 3 and 7:

Western blot - A-431 whole cell lysate (<a href='/products/cell-lysates/a-431-whole-cell-lysate-ab7909'>ab7909</a>) at 10 µg

Lanes 4 and 8:

Western blot - HEK-293 whole cell lysate (<a href='/products/cell-lysates/hek-293-whole-cell-lysate-ab7902'>ab7902</a>) at 10 µg

Predicted band size: 36 kDa

true

• WB

Unknown

Western blot - HRP Anti-GAPDH antibody - Loading Control (AB9385)

Lane 1 - 4 : GAPDH antibody - Loading Control (ab9385) at 1 ug/ml

Lane 1 : HeLa cell lysate at 20 ug
Lane 2 : A431 cell lysate at 20 ug
Lane 3 : Jurkat cell lysate at 20 ug
Lane 4 : 293 cell lysate at 20 ug

Performed under reducing conditions.

Exposure time : 1 minute

All lanes:

Western blot - HRP Anti-GAPDH antibody - Loading Control (ab9385) at 1 µg/mL

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

A431 cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

293 cell lysate at 20 µg

Predicted band size: 36 kDa

Observed band size: 38 kDa

false

Exposure time: 1min