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HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) 抗体 [M2] (ab49763)

Reviews (8) Submit a question References (88)
Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
  • ELISA - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
  • Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)

Key features and details

  • HRP Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence)
  • Suitable for: WB, ELISA
  • Reacts with: Species independent
  • Conjugation: HRP
  • Isotype: IgG1

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab245895)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]
    DDDDK tag (Binds to FLAG® tag sequence) 一次抗体 製品一覧

  • 製品の詳細

    HRP Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence)

  • 由来種

    Mouse

  • 標識

    HRP

  • アプリケーション

    適用あり: WB, ELISAmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence).
    Sequence:

    DYKDDDDK

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 3rd December 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Lyophilized:Reconstitute to 1.0 ml with distilled deionized water vortex gently and allow vial to sit on ice for 20 minutes. Reconstituted product should be stored at 4°C in the dark and be used within 3 months.

  • 保存方法

    Shipped at 4°C. Store at +4°C. Store In the Dark.

  • バッファー

    pH: 7.40
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.64% Sodium phosphate, 0.58% Sodium chloride, 1.5% BSA, 1.71% Sucrose
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    M2

  • アイソタイプ

    IgG1

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab49763の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (7)
1/500 - 1/10000.
ELISA (1)
1/20000.
特記事項
WB
1/500 - 1/10000.
ELISA
1/20000.

ターゲット情報

  • 関連性

    This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence).

  • 別名

    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK (also known as the FLAG® tag sequence) antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG® antibody
    • FLAG® tag antibody
    see all

画像

  • Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
    Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)This image is courtesy of an anonymous Abreview
    All lanes : HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763) at 1/3000 dilution

    All lanes : Saccharomyces cerevisiae Cell lysate - whole cell (S. cerevisiae cell lysate).

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 45 seconds


    Sample 1, 2, 3, 4, 5, 6 are the lysates from the strains expressing the tagged protein.

    5% milk blocking was used for 1 hour.

    See Abreview

  • ELISA - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
    ELISA - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)This image is courtesy of an anonymous Abreview

    ELISA for the detection of FLAG®-tag purified protein from Escherichia coli, using ab28947 (1/5000). Please refer to abreview for further experimental details.

    Please note that in order to show a clearer image, we have brightened the original image.

    See Abreview

  • Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
    Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)This image is courtesy of an anonymous Abreview

    Saccharomyces cerevisiae purified FLAG®-tag protein was detected by using ab49763 (1/1000). Please refer to abreview for further experimental details.

    See Abreview

参考文献 (88)

ab49763 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab49763 は 88 報の論文で使用されています。

  • Zhu W  et al. In-frame deletion of SMC5 related with the phenotype of primordial dwarfism, chromosomal instability and insulin resistance. Clin Transl Med 13:e1007 (2023). PubMed: 36627765
  • Choi YJ  et al. Lung-specific MCEMP1 functions as an adaptor for KIT to promote SCF-mediated mast cell proliferation. Nat Commun 14:2045 (2023). PubMed: 37041174
  • Bhatia V  et al. Characterization of Adenylyl Cyclase Isoform 6 Residues Interacting with Forskolin. Biology (Basel) 12:N/A (2023). PubMed: 37106773
  • Ma B  et al. Albumosomes formed by cytoplasmic pre-folding albumin maintain mitochondrial homeostasis and inhibit nonalcoholic fatty liver disease. Signal Transduct Target Ther 8:229 (2023). PubMed: 37321990
  • Meyer M  et al. Structure-function relationships of the disease-linked A218T oxytocin receptor variant. Mol Psychiatry 27:907-917 (2022). PubMed: 34980886
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-8 of 8 Abreviews or Q&A

Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (S. cerevisiae cell lysate)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
10 µg
Specification
S. cerevisiae cell lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 04 2014

Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Purified protein (NIH3T3 cells)
Gel Running Conditions
Reduced Denaturing (3-8 % Tris-Acetate Gel)
Loading amount
5e+006 cells
Treatment
10 mM Sodium Butyrate for 24 hrs, 500 ug/ml Geneticin for 48 hrs
Specification
NIH3T3 cells
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jan 07 2011

Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Escherichia coli Cell lysate - whole cell (chimeric protein)
Gel Running Conditions
Reduced Denaturing (4-12% bis tris)
Loading amount
2 µg
Specification
chimeric protein
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 17 2010

Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

 Good
Abreviews
abreview image
Application
Western blot
Sample
Chinese hamster Cell lysate - whole cell (CHO-S, transfected with plasmid containing insert)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
125000 cells
Specification
CHO-S, transfected with plasmid containing insert
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jun 15 2010

Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Escherichia coli Purified protein (BL21(DE3))
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
1 µg
Specification
BL21(DE3)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 27 2010

ELISA abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

 Excellent
Abreviews
abreview image
Application
ELISA
Sample
Escherichia coli Purified protein (Mach1 (Invitrogen))
Specification
Mach1 (Invitrogen)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Type
Direct
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 19 2009

Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Saccharomyces cerevisiae Purified protein (BJ5453)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
1 µg
Specification
BJ5453
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jan 14 2009

Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Escherichia coli Purified protein (Mach1)
Gel Running Conditions
Reduced Denaturing (12% SDS PAGE)
Loading amount
0.01 µg
Specification
Mach1
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 16 2008

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