HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) 抗体 (ab2493)
Key features and details
- HRP Rabbit polyclonal to DDDDK tag (Binds to FLAG® tag sequence)
- Suitable for: ELISA, WB
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody
DDDDK tag (Binds to FLAG® tag sequence) 一次抗体 製品一覧 -
製品の詳細
HRP Rabbit polyclonal to DDDDK tag (Binds to FLAG® tag sequence) -
由来種
Rabbit -
標識
HRP -
アプリケーション
適用あり: ELISA, WBmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence).
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特記事項
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2493の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ELISA |
1/5000 - 1/20000.
|
|
WB | (1) |
1/1000 - 1/10000.
|
特記事項 |
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ELISA
1/5000 - 1/20000. |
WB
1/1000 - 1/10000. |
ターゲット情報
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関連性
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence). -
別名
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
see all
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab2493 は 4 報の論文で使用されています。
- Reed A et al. TMEM164 is an acyltransferase that forms ferroptotic C20:4 ether phospholipids. Nat Chem Biol 19:378-388 (2023). PubMed: 36782012
- Bernard PE et al. Single Amino Acid Replacements in RocA Disrupt Protein-Protein Interactions To Alter the Molecular Pathogenesis of Group A Streptococcus. Infect Immun 88:N/A (2020). PubMed: 32817331
- Kling E et al. Convergent evolution of integration site selection upstream of tRNA genes by yeast and amoeba retrotransposons. Nucleic Acids Res 46:7250-7260 (2018). PubMed: 29945249
- Belashov IA et al. Structure of HIV TAR in complex with a Lab-Evolved RRM provides insight into duplex RNA recognition and synthesis of a constrained peptide that impairs transcription. Nucleic Acids Res 46:6401-6415 (2018). PubMed: 29961805