AB201678

HRP Anti-CNPase 抗体 [11-5B]

HRP Anti-CNPase antibody [11-5B]

KO Validated
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(1 Publication)

Anti-CNPase antibody [11-5B] conjugated to HRP - suitable for IHC-P, Western blot, and reacts with mouse, human, rat samples. Clone [11-5B] is a mouse monoclonal antibody cited in 5 publications.

別名を表示する

CNP, CNPase

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CNPase antibody [11-5B] (AB201678)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CNPase antibody [11-5B] (AB201678)

IHC image of CNPase staining in a section of formalin-fixed paraffin-embedded human normal cerebral cortex*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab201678 at 1/100 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Lab

Western blot - HRP Anti-CNPase antibody [11-5B] (AB201678)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab201678 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-CNPase antibody [11-5B] (ab201678) at 1/5000 dilution

Lane 1:

Brain (Human) Tissue Lysate - adult normal tissue at 10 µg

Lane 2:

Spinal Cord (Human) Tissue Lysate - adult normal tissue at 10 µg

Lane 3:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 4:

Spinal Cord (Mouse) Tissue Lysate at 10 µg

Lane 5:

Brain (Rat) Tissue Lysate at 10 µg

Lane 6:

Spinal Cord (Rat) Tissue Lysate at 10 µg

Predicted band size: 48 kDa

Observed band size: 48 kDa

true

Exposure time: 6s

Lab

Western blot - HRP Anti-CNPase antibody [11-5B] (AB201678)

ab201678 was shown to specifically react with CNPase in wild-type HAP1 cells as signal was lost in CNPase knockout cells. Wild-type and CNPase knockout samples were subjected to SDS-PAGE. ab201678 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor^® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

All lanes:

Western blot - HRP Anti-CNPase antibody [11-5B] (ab201678) at 1/5000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

CNPase knockout HAP1 whole cell lysate at 20 µg

Predicted band size: 48 kDa

Observed band size: 48 kDa

false

Exposure time: 20min

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

11-5B

アイソタイプ

IgG1

標識

HRP

励起波長/蛍光波長

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification

バッファー組成

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Stable for 12 months at -20°C|Store in the dark

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Myelin-associated enzyme that catalyzes the phosphodiester hydrolysis of 2',3'-cyclic nucleotides to 2'-nucleotides. In the mitochondria, regulates the functioning of the mitochondrial permeability transition pore (mPTP), and thus is involved in the mechanisms of cell death, both apoptosis and necrosis. Acts as an antiviral factor by suppressing the assembly of SARS-CoV-2 virions (PubMed : 38117830).. Acts as an antiviral factor by suppressing the assembly of HIV-1 virions through direct interaction with GAG.

See full target information CNP

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 130:1377-1391 PubMed31877112

2019

Microphthalmia transcription factor expression contributes to bone marrow failure in Fanconi anemia.

Applications

Unspecified application

Species

Unspecified reactive species

Alessia Oppezzo,Julie Bourseguin,Emilie Renaud,Patrycja Pawlikowska,Filippo Rosselli

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-CNPase 抗体 [11-5B]

HRP Anti-CNPase antibody [11-5B]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CNPase antibody [11-5B] (AB201678)

Western blot - HRP Anti-CNPase antibody [11-5B] (AB201678)

Western blot - HRP Anti-CNPase antibody [11-5B] (AB201678)

関連する標識済み抗体及び組成の異なる製品 (7)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (1)

Abcam product promise