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HRP Anti-CHREBP 抗体 (ab81958)

Reviews (1)Q&A (1)References (5)
Western blot - HRP Anti-CHREBP antibody (ab81958)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CHREBP antibody (ab81958)
  • Immunocytochemistry/ Immunofluorescence - HRP Anti-CHREBP antibody (ab81958)
  • Western blot - HRP Anti-CHREBP antibody (ab81958)

Key features and details

  • HRP Rabbit polyclonal to CHREBP
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Conjugation: HRP
  • Isotype: IgG

製品の概要

  • 製品名

    HRP Anti-CHREBP antibody
    CHREBP 一次抗体 製品一覧

  • 製品の詳細

    HRP Rabbit polyclonal to CHREBP

  • 由来種

    Rabbit

  • 標識

    HRP

  • 特異性

    A customer has observed some cross-reactivity with MLX-interacting protein (Q9HAP2). Although the exact sequence is proprietary, the immunogen for ab81958 shows 75% homology with this protein.

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide within Human CHREBP aa 800 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_116569.1
    Database link: Q9NP71

  • ポジティブ･コントロール

    • Human hepatocyte lysate, human hepatocytes, liver nuclear extracts from well fed rats, and colon epithelium.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab81958の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
Use at an assay dependent dilution. Predicted molecular weight: 95 kDa.
IHC-P
Use at an assay dependent dilution.
ICC/IF
1/100 - 1/500.
特記事項
WB
Use at an assay dependent dilution. Predicted molecular weight: 95 kDa.
IHC-P
Use at an assay dependent dilution.
ICC/IF
1/100 - 1/500.

ターゲット情報

  • 機能

    Transcriptional repressor. Binds to the canonical and non-canonical E box sequences 5'-CACGTG-3'.

  • 組織特異性

    Expressed in liver, heart, kidney, cerebellum and intestinal tissues.

  • 関連疾患

    Note=WBSCR14 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of WBSCR14 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.

  • 配列類似性

    Contains 1 basic helix-loop-helix (bHLH) domain.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • carbohydrate response element binding protein antibody
      • bHLHd14 antibody
      • Carbohydrate responsive element binding protein antibody
      • Class D basic helix-loop-helix protein 14 antibody
      • MIO antibody
      • MLX interacting protein like antibody
      • Mlx interactor antibody
      • MLX-interacting protein-like antibody
      • MLXIPL antibody
      • MONDOB antibody
      • WBS14_HUMAN antibody
      • WBSCR 14 antibody
      • WBSCR14 antibody
      • Williams Beuren syndrome chromosome region 14 antibody
      • Williams Beuren syndrome chromosome region 14 protein antibody
      • Williams-Beuren syndrome chromosomal region 14 protein antibody
      • WS basic helix loop helix leucine zipper protein antibody
      • WS basic-helix-loop-helix leucine zipper protein antibody
      • WS bHLH antibody
      • WS-bHLH antibody
      see all

    画像

    • Western blot - HRP Anti-CHREBP antibody (ab81958)
      Western blot - HRP Anti-CHREBP antibody (ab81958)
      anti CHREBP (unconjugated form of ab81958) + human hepatocyte lysate at 20 µg

      Predicted band size: 95 kDa
      Observed band size: 95 kDa



      5-10 second film exposure.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CHREBP antibody (ab81958)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CHREBP antibody (ab81958)

      anti CHREBP (unconjugated form of ab81958), staining CHREBP in human colon epithelium (40X) by immunohistochemistry.

    • Immunocytochemistry/ Immunofluorescence - HRP Anti-CHREBP antibody (ab81958)
      Immunocytochemistry/ Immunofluorescence - HRP Anti-CHREBP antibody (ab81958)
      anti CHREBP (unconjugated form of ab81958), at a 1/100 dilution, staining CHREBP in human hepatocyte by Immunofluorescence. Overlay staining of ChREBP (rhodamine red) and DAPI nuclear staining (blue).
    • Western blot - HRP Anti-CHREBP antibody (ab81958)
      Western blot - HRP Anti-CHREBP antibody (ab81958)
      All lanes : anti CHREBP (unconjugated form of ab81958), at 1/1000 dilution

      Lane 1 : liver nuclear extracts from well fed rats at 10 µg
      Lane 2 : liver nuclear extracts from well fed rats at 5 µg
      Lane 3 : liver nuclear extracts from well fed rats at 1 µg
      Lane 4 : liver nuclear extracts from well fed rats at 0.5 µg

      Predicted band size: 95 kDa
      Observed band size: 95 kDa



      7% SDS gel.

    参考文献 (5)

    ab81958 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab81958 は 5 報の論文で使用されています。

    • Zhang J  et al. TXNIP induced by MondoA, rather than ChREBP, suppresses cervical cancer cell proliferation, migration and invasion. J Biochem 167:371-377 (2020). PubMed: 31782782
    • Nguyen P  et al. Elimination of Age-Associated Hepatic Steatosis and Correction of Aging Phenotype by Inhibition of cdk4-C/EBPa-p300 Axis. Cell Rep 24:1597-1609 (2018). PubMed: 30089269
    • Sillam-Dussès D  et al. The role of the glucose-sensing transcription factor carbohydrate-responsive element-binding protein pathway in termite queen fertility. Open Biol 6:N/A (2016). WB, IHC . PubMed: 27249798
    • Sujishi T  et al. Sitagliptin can inhibit the development of hepatic steatosis in high-fructose diet-fed ob/ob mice. J Clin Biochem Nutr 57:244-53 (2015). WB . PubMed: 26566312
    • Huang H  et al. Inhibitors of Fatty Acid Synthesis Induce PPAR a -Regulated Fatty Acid ß -Oxidative Genes: Synergistic Roles of L-FABP and Glucose. PPAR Res 2013:865604 (2013). PubMed: 23533380

    レビューと Q&A

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    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-CHREBP antibody (HRP)

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (liver and fat tissue)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    50 µg
    Specification
    liver and fat tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Feb 17 2016

    Question

    je n'ai jamais utilisé un ab couplé HRP. faut-il un systeme de révélation particulier?

    Read More
    Answer


    Pour visualiser l’HRP il faut ajouter un substrat qui, lorsqu'il est oxydé par l'HRP, change de couleur ou produit de la lumière qui peut être détecté.
    De nombreux substrats pour HRP peuvent être utilisés :
    HRP catalyse la conversion des substrats chromogènes (par exemple, TMB, DAB, ABTS) en produits colorés.
    HRP produit de la lumière quand il agit sur des substrats chimioluminescent, par exemple ECL.
    Dans notre catalogue nous vendons plusieurs de ces substrats. Par exemple :
    Pour l’immunohistochimie :
    DAB Substrate Kit (ab64238)
    DAB substrate kit (ab94665)
    Pour le western blot :
    ECL Western Blotting Substrate Kit - 50 Tests (ab65623)
    Chemiluminescent HRP Substrate (membrane) (ab5801)
    Optiblot ECL Ultra Detect Kit (1.2pg-2ng) (ab133409)
    Optiblot ECL Detect Kit (23pg-187ng) (ab133406)
    Optiblot ECL Max Detect Kit (4.6pg-4.7ng) (ab133408)

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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