AB81958

HRP Anti-CHREBP 抗体

Name: HRP Anti-CHREBP 抗体 (ab81958) | Abcam
Brand: Abcam Limited
SKU: AB81958
Price: 78000 JPY
Availability: InStock
Rating: 4 (1 reviews)

HRP Anti-CHREBP antibody

(1 Review)

(7 Publications)

Rabbit Polyclonal CHREBP antibody - conjugated to HRP. Suitable for WB, IHC-P, ICC and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human MLXIPL aa 800 to C-terminus.

別名を表示する

BHLHD14, MIO, WBSCR14, MLXIPL, Carbohydrate-responsive element-binding protein, ChREBP, Class D basic helix-loop-helix protein 14, MLX interactor, MLX-interacting protein-like, WS basic-helix-loop-helix leucine zipper protein, Williams-Beuren syndrome chromosomal region 14 protein, bHLHd14, WS-bHLH

3 Images

Immunocytochemistry/ Immunofluorescence - HRP Anti-CHREBP antibody (AB81958)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - HRP Anti-CHREBP antibody (AB81958)

anti CHREBP (unconjugated form of ab81958), at a 1/100 dilution, staining CHREBP in human hepatocyte by Immunofluorescence. Overlay staining of ChREBP (rhodamine red) and DAPI nuclear staining (blue).

Unknown

Western blot - HRP Anti-CHREBP antibody (AB81958)

5-10 second film exposure.

All lanes:

anti CHREBP (unconjugated form of ab81958)

All lanes:

human hepatocyte lysate at 20 µg

Predicted band size: 93 kDa

Observed band size: 95 kDa

false

Unknown

Western blot - HRP Anti-CHREBP antibody (AB81958)

7% SDS gel.

All lanes:

anti CHREBP (unconjugated form of ab81958), at 1/1000 dilution

Lane 1:

liver nuclear extracts from well fed rats at 10 µg

Lane 2:

liver nuclear extracts from well fed rats at 5 µg

Lane 3:

liver nuclear extracts from well fed rats at 1 µg

Lane 4:

liver nuclear extracts from well fed rats at 0.5 µg

Predicted band size: 93 kDa

Observed band size: 95 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

標識

HRP

励起波長/蛍光波長

キャリアフリー

交差種

Human

アプリケーション

IHC-P, WB, ICC

applications

免疫原

Synthetic Peptide within Human MLXIPL aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NP71

特異性

A customer has observed some cross-reactivity with MLX-interacting protein (Q9HAP2). Although the exact sequence is proprietary, the immunogen for ab81958 shows 75% homology with this protein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/500", "ICC-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

Purified rabbit antisera.

バッファー組成

Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Glucose-responsive transcription activator that regulates fatty acid synthesis and glycolysis. Key determinant of systemic insulin sensitivity and glucose homeostasis. Important for the expression of fatty acid synthetic enzymes, including PC/Pcx, APOC4/Acl, ACACA/Acc1 and FASN/Fas (By similarity). Important for glucose-induced expression of L-type pyruvate kinase/PKLR (By similarity). Binds to the canonical and non-canonical E box DNA sequences 5'-CACGTG-3' and 5'-CACGCG-3' (By similarity). May also act as a transcriptional repressor (By similarity).

See full target information MLXIPL

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Aquaculture nutrition 2023:6672985 PubMed37520290

2023

Sequential Activations of ChREBP and SREBP1 Signals Regulate the High-Carbohydrate Diet-Induced Hepatic Lipid Deposition in Gibel Carp ().

Applications

Unspecified application

Species

Unspecified reactive species

Yulong Gong,Longwei Xi,Yulong Liu,Qisheng Lu,Zhimin Zhang,Haokun Liu,Junyan Jin,Yunxia Yang,Xiaoming Zhu,Shouqi Xie,Dong Han

The Journal of nutrition 150:1790-1798 PubMed32470978

2020

Dietary Glucose Increases Glucose Absorption and Lipid Deposition via SGLT1/2 Signaling and Acetylated ChREBP in the Intestine and Isolated Intestinal Epithelial Cells of Yellow Catfish.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Zhao,Shui-Bo Yang,Guang-Hui Chen,Yi-Huan Xu,Yi-Chuang Xu,Zhi Luo

Journal of biochemistry 167:371-377 PubMed31782782

2019

TXNIP induced by MondoA, rather than ChREBP, suppresses cervical cancer cell proliferation, migration and invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Junhua Zhang,Xingbo Tian,Huifang Yin,Songshu Xiao,Shuijing Yi,Youzhong Zhang,Fei Zeng

Cell reports 24:1597-1609 PubMed30089269

2018

Elimination of Age-Associated Hepatic Steatosis and Correction of Aging Phenotype by Inhibition of cdk4-C/EBPα-p300 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Phuong Nguyen,Leila Valanejad,Ashley Cast,Mary Wright,Jose M Garcia,Hashem B El-Serag,Rebekah Karns,Nikolai A Timchenko

Open biology 6: PubMed27249798

2016

The role of the glucose-sensing transcription factor carbohydrate-responsive element-binding protein pathway in termite queen fertility.

Applications

IHC, WB

Species

Unspecified reactive species, Unspecified reactive species

David Sillam-Dussès,Robert Hanus,Michael Poulsen,Virginie Roy,Maryline Favier,Mireille Vasseur-Cognet

Journal of clinical biochemistry and nutrition 57:244-53 PubMed26566312

2015

Sitagliptin can inhibit the development of hepatic steatosis in high-fructose diet-fed ob/ob mice.

Applications

Species

Unspecified reactive species

Tetsuya Sujishi,Shinya Fukunishi,Masaaki Ii,Ken Nakamura,Keisuke Yokohama,Hideko Ohama,Yusuke Tsuchimoto,Akira Asai,Yasuhiro Tsuda,Kazuhide Higuchi

PPAR research 2013:865604 PubMed23533380

2013

Inhibitors of Fatty Acid Synthesis Induce PPAR α -Regulated Fatty Acid β -Oxidative Genes: Synergistic Roles of L-FABP and Glucose.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Huang,Avery L McIntosh,Gregory G Martin,Anca D Petrescu,Kerstin K Landrock,Danilo Landrock,Ann B Kier,Friedhelm Schroeder

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-CHREBP 抗体

HRP Anti-CHREBP antibody

Immunocytochemistry/ Immunofluorescence - HRP Anti-CHREBP antibody (AB81958)

Western blot - HRP Anti-CHREBP antibody (AB81958)

Western blot - HRP Anti-CHREBP antibody (AB81958)

Key facts

宿主種

クローン性

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

製品プロトコール

ターゲットの情報

文献 (7)

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Proteins & Peptides

Abcam product promise