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AB202364

HRP Anti-Cdc37 (phospho S13) 抗体 [EPR4879]

HRP Anti-Cdc37 (phospho S13) antibody [EPR4879]

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(1 Publication)

Rabbit Recombinant Monoclonal CDC37 phospho S13 antibody - conjugated to HRP. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

別名を表示する

CDC37A, CDC37, Hsp90 co-chaperone Cdc37, Hsp90 chaperone protein kinase-targeting subunit, p50Cdc37

1 Images
Western blot - HRP Anti-Cdc37 (phospho S13) antibody [EPR4879] (AB202364)
  • WB

Lab

Western blot - HRP Anti-Cdc37 (phospho S13) antibody [EPR4879] (AB202364)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab202864 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab202364) at 1/5000 dilution

Lane 1:

Untreated HeLa (Human epithelial carcinoma cell line) whole cell lysate at 10 µg

Lane 2:

HeLa (Human epithelial carcinoma cell line) whole cell lysate serum starved and Calyculin A treated at 10 µg

Lane 3:

HeLa (Human epithelial carcinoma cell line) whole cell lysate, serum starved and Calyculin A treated, then membrane was then incubated with Alkaline phosphatase at 10 µg

Predicted band size: 44 kDa

Observed band size: 44 kDa

true

Exposure time: 3min

関連する標識済み抗体及び組成の異なる製品 (2)

  • Unconjugated

    Anti-Cdc37 (phospho S13) antibody [EPR4879]

  • Carrier free

    Anti-Cdc37 (phospho S13) antibody [EPR4879] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR4879

アイソタイプ

IgG

標識

HRP

励起波長/蛍光波長
キャリアフリー

No

交差種

Human, Human, Mouse, Rat

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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代替製品

Primary Antibodies

AB115166

Anti-Cdc37 antibody

Immunoprecipitation - Anti-Cdc37 antibody (AB115166)

  • 5
  • 1
Primary Antibodies

AB109419

Anti-Cdc37 antibody [EPR4934]

RabMAb|Recombinant|Trial Size|Trial Size

Immunoprecipitation - Anti-Cdc37 antibody [EPR4934] (AB109419)

  • 0
  • 0
Primary Antibodies

AB108305

Anti-Cdc37 antibody [EPR4935]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc37 antibody [EPR4935] (AB108305)

  • 0
  • 0

組合せ製品

Primary Antibodies

AB109419

Anti-Cdc37 antibody [EPR4934]

RabMAb|Recombinant|Trial Size

Immunoprecipitation - Anti-Cdc37 antibody [EPR4934] (AB109419)

  • 0
  • 0
Primary Antibodies

AB33279

FITC Anti-P-Selectin (CD62P) antibody [AK-6]

  • 0
  • 0
Primary Antibodies

AB203462

HRP Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)]

RabMAb|Recombinant

Western blot - HRP Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (AB203462)

  • 0
  • 0
Primary Antibodies

AB83036

Anti-AHA1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-AHA1 antibody (AB83036)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Stable for 12 months at -20°C|Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The Cdc37 protein also known as cell division cycle 37 homolog is a molecular chaperone that assists in the proper folding and stabilization of protein kinases. With a mass of approximately 44 kDa Cdc37 is widely expressed in mammalian tissues. It interacts directly with other proteins aiding in their stabilization and activity. Functioning closely with Hsp90 Cdc37 forms a chaperone complex critical for the maturation of several protein kinases.
Biological function summary

Cdc37 serves as a co-chaperone with Hsp90 in regulating protein kinase activity by maintaining their functional conformation and stability. This protein acts mainly as a kinase-specific adapter that recruits kinases to the Hsp90 chaperone system forming a protein complex essential for the proper functioning of key signaling molecules. Its role is vital in signal transduction and cellular growth regulation ensuring proper cell cycle progression and response to external stimuli.

Pathways

Cdc37 plays a significant role in the PI3K/AKT and MAPK signaling pathways. In these pathways Cdc37 assists in the proper activation and function of various kinases such as AKT and RAF which are important for cell growth survival and proliferation. Its partnership with Hsp90 in these pathways underlines its importance in maintaining kinase activity ensuring that signaling cascades proceed correctly for normal cellular function.

Cdc37 has been implicated in cancer and neurodegenerative diseases like Alzheimer's. Overexpression or dysfunction of Cdc37 can lead to improper activation of oncogenic kinases contributing to tumor progression. Additionally the protein's association with Hsp90 highlights its potential involvement in Alzheimer's where protein misfolding and stability are disrupted making Cdc37 a target of interest for therapeutic intervention in these conditions.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity (PubMed : 8666233). Inhibits HSP90AA1 ATPase activity (PubMed : 23569206).
See full target information CDC37 pS13
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Oncogene : PubMed33452458

2021

NOTCH3, a crucial target of miR-491-5p/miR-875-5p, promotes gastric carcinogenesis by upregulating PHLDB2 expression and activating Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Kang,Jinglin Zhang,Tingting Huang,Yuhang Zhou,Chi Chun Wong,Ronald C K Chan,Yujuan Dong,Feng Wu,Bin Zhang,William K K Wu,Michael W Y Chan,Alfred S L Cheng,Jun Yu,Nathalie Wong,Kwok Wai Lo,Ka Fai To
View all publications
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Abcam product promise

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