AB199219

HRP Anti-CD90 / Thy1 抗体 [MRC OX-7]

Name: HRP Anti-CD90 / Thy1 抗体 [MRC OX-7] (ab199219) | Abcam
Brand: Abcam Limited
SKU: AB199219
Price: 81000 JPY
Availability: InStock

HRP Anti-CD90 / Thy1 antibody [MRC OX-7]

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(1 Publication)

Anti-CD90 / Thy1 antibody [MRC OX-7] conjugated to HRP - suitable for Western blot, and reacts with rat samples. Clone [MRC OX-7] is a mouse monoclonal antibody cited in 73 publications.

別名を表示する

CD90, Thy-1, Thy1, Thy-1 membrane glycoprotein, Thy-1 antigen

1 Images

Lab

Western blot - HRP Anti-CD90 / Thy1 antibody [MRC OX-7] (AB199219)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab199219 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab199219) at 1/5000 dilution

Lane 1:

Brain (Rat) Tissue Lysate at 10 µg

Lane 2:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 17 kDa

Observed band size: 25 kDa

true

Exposure time: 30s

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

MRC OX-7

アイソタイプ

IgG1

軽鎖のタイプ

kappa

標識

HRP

励起波長/蛍光波長

キャリアフリー

交差種

Rat

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p>Observed molecular weight may vary depending on the glycosylation level of the target.</p>" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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製品の詳細

The affinity of the Fab' of MRC OX-7 for rat Thy-1 is 3 x 10⁹m^-1 and for mouse Thy-1.1 is 3 x 10⁸m^-1.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification

バッファー組成

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Store in the dark

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD90 also known as Thy1 is a glycoprotein with a molecular mass of approximately 25-37 kDa. It is highly conserved across species and found on the surface of various cell types including fibroblasts neurons and endothelial cells. CD90 is expressed in these cells in tissues such as the brain thymus and lungs. Researchers use anti-CD90 antibodies for detection often in combination with CD90 FITC in immunofluorescence assays. CD90 serves as a useful cell marker particularly in identifying mesenchymal stem cells (MSCs).

Biological function summary

CD90 plays a role in cell-cell and cell-matrix interactions contributing to cellular adhesion migration and signal transduction. It functions as part of a complex involving integrins which mediate its interaction with the extracellular matrix. This protein also influences T-cell activation reflecting its importance in immune response regulation. Its presence aids in modulating response to injury and promoting tissue repair due to its association with stem and progenitor cells.

Pathways

CD90 engagement affects focal adhesion and MAPK signaling pathways. It interacts with integrins like αvβ3 and α5β1 influencing cell movement and proliferation processes. CD90 involvement in these pathways allows it to regulate responses important for cell survival and communication within various tissues. These interactions highlight the protein's role in maintaining cellular homeostasis and regulating physiological processes.

CD90 has associations with fibrosis and cancer. In fibrotic conditions CD90 expression affects fibroblast activity influencing tissue scarring in organs such as the liver and lungs. In cancer CD90 modifies tumor progression and metastasis by impacting cell adhesion and migration with connections to proteins like TGF-beta which further drive these processes. Understanding CD90 in disease contexts facilitates the development of therapeutic strategies aimed at modulating its activity.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

See full target information Thy1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Pediatrics international : official journal of the Japan Pediatric Society 64:e15159 PubMed35727889

2022

The histone deacetylase inhibitor OBP-801 has in vitro/in vivo anti-neuroblastoma activity.

Applications

Unspecified application

Species

Unspecified reactive species

Daisuke Kaneda,Tomoko Iehara,Ken Kikuchi,Yohei Sugimoto,Norio Nakagawa,Shigeki Yagyu,Mitsuru Miyachi,Eiichi Konishi,Toshiyuki Sakai,Hajime Hosoi

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-CD90 / Thy1 抗体 [MRC OX-7]

HRP Anti-CD90 / Thy1 antibody [MRC OX-7]

Western blot - HRP Anti-CD90 / Thy1 antibody [MRC OX-7] (AB199219)

関連する標識済み抗体及び組成の異なる製品 (5)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

軽鎖のタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

Abcam product promise