AB173840

HRP Anti-beta Tubulin 抗体 [BT7R]

Name: HRP Anti-beta Tubulin 抗体 [BT7R] (ab173840) | Abcam
Brand: Abcam Limited
SKU: AB173840
Price: 76000 JPY
Availability: InStock

HRP Anti-beta Tubulin antibody [BT7R] - Loading Control

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(3 Publications)

Mouse Monoclonal Beta-5-tubulin antibody - conjugated to HRP. Suitable for WB and reacts with Mouse, Rat, African green monkey, Human, Dog, Primates samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TUBB conjugated to Keyhole Limpet Haemocyanin.

別名を表示する

TUBB5, OK/SW-cl.56, TUBB, Tubulin beta chain, Tubulin beta-5 chain

2 Images

Supplier Data

Western blot - HRP Anti-beta Tubulin antibody [BT7R] - Loading Control (AB173840)

Blocking buffer : 5% milk.

All lanes:

Western blot - HRP Anti-beta Tubulin antibody [BT7R] - Loading Control (ab173840) at 1 µg/mL

Lane 1:

A549 (Human adenocarcinomic alveolar basal epithelial cells) at 30 µg

Lane 2:

COS-7 (African green monkey kidney fibroblast cells) at 30 µg

Lane 3:

MDCK (Madin-Darby Canine Kidney cells) at 30 µg

Lane 4:

C2C12 (Mouse myoblast cells) at 30 µg

Lane 5:

MDA-MB-231 (Human breast cancer cells) at 30 µg

Lane 6:

PC-12 (Embryonic Rat adrenal medulla cells) at 30 µg

Lane 7:

RSC96 at 30 µg

Lane 8:

Mouse lung tissue at 30 µg

Predicted band size: 49 kDa

Observed band size: 50 kDa

false

Supplier Data

Western blot - HRP Anti-beta Tubulin antibody [BT7R] - Loading Control (AB173840)

4-20% Tris-HCl polyacrylamide gel.

All lanes:

Western blot - HRP Anti-beta Tubulin antibody [BT7R] - Loading Control (ab173840) at 1/1000 dilution

Lane 1:

A431 cell lysate at 50 µg

Lane 2:

U2OS cell lysate at 50 µg

Lane 3:

293T cell lysate at 50 µg

Lane 4:

Jurkat cell lysate at 50 µg

Lane 5:

HeLa cell lysate at 50 µg

Lane 6:

COS7 cell lysate at 50 µg

Lane 7:

C2C12 cell lysate at 50 µg

Lane 8:

NRK cell lysate at 50 µg

Predicted band size: 49 kDa

true

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

BT7R

アイソタイプ

IgG2a

標識

HRP

励起波長/蛍光波長

キャリアフリー

交差種

Mouse, Rat, Dog, Human, Primates, African green monkey

アプリケーション

applications

免疫原

Synthetic Peptide within Human TUBB conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P07437

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Primates": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

バッファー組成

Constituents: PBS, 0.07% Kathon

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Beta tubulin also known as beta-tubulin or β-tubulin is a globular protein with a molecular weight of approximately 50 kDa. It is a major component of microtubules which are cylindrical structures vital for maintaining cell shape and enabling intracellular transport. This protein is expressed widely in eukaryotic cells and plays a role in mitosis and meiosis by forming the spindle apparatus important for chromosome separation during cell division. The size of beta tubulin allows it to effectively polymerize with alpha tubulin forming heterodimers which are the building blocks of the microtubule network.

Biological function summary

This protein contributes significantly to the dynamic instability of microtubules allowing rapid assembly and disassembly which is essential for cellular processes like motility signaling and maintaining the cell’s architecture. Beta tubulin operates as part of the tubulin family which includes several related proteins within microtubule structures. The beta-tubulin molecules in microtubules are critical for the interactions with microtubule-associated proteins (MAPs) and motor proteins such as kinesin and dynein influencing trafficking and positioning of organelles within the cell.

Pathways

Beta tubulin plays key roles in the mitotic spindle assembly checkpoint ensuring accurate chromosome segregation. It is actively involved in the microtubule pathway and has associations with signaling pathways such as the Wnt signaling pathway which affects cell growth and differentiation. Through these pathways beta tubulin interacts with proteins like tau and MAP2 which stabilize microtubules to control their functional dynamics within the cell.

Mutations or dysregulations in beta tubulin can affect neurological and proliferative conditions such as neurodegenerative diseases and cancer. Beta tubulin is notably linked to Alzheimer's disease where tau protein tangles disrupt normal microtubule function. It also connects to diseases like paclitaxel-resistant cancer where altered beta tubulin isoform expression can lead to chemotherapy resistance. The interaction of beta tubulin with tau and alpha tubulin provides further insight into etiological mechanisms of these conditions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TUBB

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Viruses 17: PubMed40285013

2025

Characterization of Nanobody Binding to Distinct Regions of the SARS-CoV-2 Spike Protein by Flow Virometry.

Applications

Unspecified application

Species

Unspecified reactive species

Mariam Maltseva,Martin A Rossotti,Jamshid Tanha,Marc-André Langlois

Journal of thrombosis and haemostasis : JTH 18:2976-2986 PubMed32692888

2020

The role of the androgen receptor in prostate cancer-induced platelet aggregation and platelet-induced invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Jan K Rudzinski,Natasha P Govindasamy,John D Lewis,Paul Jurasz

Molecular medicine reports 14:2405-14 PubMed27484316

2016

Proteomic analysis of the effects of CSF-1 and IL-1α on dental follicle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chanchan Chen,Nan Xie,Junqi Ling,Yu Du,Haijing Gu

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-beta Tubulin 抗体 [BT7R]

HRP Anti-beta Tubulin antibody [BT7R] - Loading Control

Western blot - HRP Anti-beta Tubulin antibody [BT7R] - Loading Control (AB173840)

Western blot - HRP Anti-beta Tubulin antibody [BT7R] - Loading Control (AB173840)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

バッファー組成

出荷温度

短期保存温度

長期保存温度

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise