AB20272

HRP Anti-beta Actin 抗体 [mAbcam 8226] - Loading Control

Name: HRP Anti-beta Actin 抗体 [mAbcam 8226] - Loading Control (ab20272) | Abcam
Brand: Abcam Limited
SKU: AB20272
Price: 59000 JPY
Availability: InStock
Rating: 5 (18 reviews)

HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control

(18 Reviews)

(377 Publications)

Anti-beta Actin antibody [mAbcam 8226] conjugated to HRP - suitable for Western blot, and reacts with mouse, human, rat samples. Clone [mAbcam 8226] is a mouse monoclonal antibody cited in 13 publications.

別名を表示する

Beta-actin, ACTB

3 Images

Unknown

Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (AB20272)

All lanes:

Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) at 1/5000 dilution

Lane 1:

human testis lysate at 20 µg

Lane 2:

human brain lysate at 20 µg

Lane 3:

human tonsil lysate at 20 µg

Lane 4:

mouse brain lysate at 20 µg

Lane 5:

mouse spleen lysate at 20 µg

Predicted band size: 41 kDa

true

Exposure time: 2min

Project

Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (AB20272)

The membrane 1-4 was blocked in 5% BSA (1 hour). The membrane 5-8 was blocked in 5% MILK (1 hour). Abcam routinely uses 5% BSA to block, however following recent customer feedback our labs investigated the effect of 5% milk blocking. We can now confirm that milk is not a suitable blocking agent for this antibody and significantly decreases the signal on the membrane.

All lanes:

Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) at 1/5000 dilution

Lanes 1 and 5:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lanes 2 and 6:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lanes 3 and 7:

Western blot - NIH/3T3 whole cell lysate (<a href='/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>) at 10 µg

Lanes 4 and 8:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 41 kDa

Observed band size: 42 kDa

true

Exposure time: 1min

Project

Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (AB20272)

All lanes:

Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) at 1/5000 dilution

Lane 1:

HeLa whole cell lysate at 20 µg

Lane 2:

3T3 whole cell lysate at 20 µg

Lane 3:

Rat brain lysate at 20 µg

Predicted band size: 41 kDa

Observed band size: 42 kDa

true

Exposure time: 30s

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

mAbcam 8226

アイソタイプ

IgG1

軽鎖のタイプ

kappa

標識

HRP

励起波長/蛍光波長

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "Abcam strongly recommends blocking and incubation using 5% BSA as we have found that use of 5% milk seems to drastically reduce the intensity of the signal obtained. Please refer to WB image for further details." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "Abcam strongly recommends blocking and incubation using 5% BSA as we have found that use of 5% milk seems to drastically reduce the intensity of the signal obtained. Please refer to WB image for further details." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "Abcam strongly recommends blocking and incubation using 5% BSA as we have found that use of 5% milk seems to drastically reduce the intensity of the signal obtained. Please refer to WB image for further details." }, "African green monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Drosophila melanogaster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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製品の詳細

What is this antibody validated in?
HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) is a mouse monoclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rat samples.

What is the molecular weight of beta Actin?
Anti-beta Actin [mAbcam 8226] - Loading Control HRP (ab20272) is often used as a loading control in Western blot. It specifically detects a band for beta Actin (UniProt: P60709) at a molecular weight of 42kDa.

Trusted by the scientific community
Anti-beta Actin [mAbcam 8226] - Loading Control HRP (ab20272) was first used in a scientific publication in 2005 and has been cited over 290 times in peer-reviewed journals.

Reviewed by scientists
Anti-beta Actin [mAbcam 8226] - Loading Control HRP (ab20272) has over 15 independent reviews from customers.

Related products
Antibody clone mAbcam 8226 is also available pre-conjugated to a variety of labels for your convenience – Anti-beta Actin HRP [mAbcam 8226] - Loading Control (ab20272).

Other related products
We have a range of other formats of antibody clone [mAbcam 8226] also available for your convenience: ab8226, HRP - ab20272, Alexa Fluor® 680 - ab184092, Alexa Fluor® 790 - ab184576, Carrier free - ab264083

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出荷温度及び保存条件

製品の状態

Liquid

精製度

IgG fraction

バッファー組成

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Store in the dark

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.

Biological function summary

Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.

Pathways

Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.

Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed : 25255767, PubMed : 29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed : 29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed : 29925947). Plays a role in the assembly of the gamma-tubulin ring complex (gTuRC), which regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments (PubMed : 39321809, PubMed : 38609661). Part of the ACTR1A/ACTB filament around which the dynactin complex is built (By similarity). The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).

See full target information ACTB

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文献 (377)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 15:9580-9600 PubMed41041054

2025

Macrophage-TREM2 promotes cardiac repair by restricting the infiltration of CD8 T cells via CXCL16-CXCR6 axis after myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Linlin Zhang,Sheng Wang,Yu Ding,Tao Zheng,Jie Sheng,Yanshan Chen,Zhiyue Wang,Ximei Dai,Canbiao Wang,Long Ma,Jing Pan,Yunming Zhang,Longjiang Zhang

Molecular medicine reports 32: PubMed40999987

2025

Curcumin exerts therapeutic effects on colorectal cancer by inducing pyroptosis through caspase‑1 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajie Zhu,Liangjun Yang,Zheng Fang,Jiabin Chen,Yeqian Wu,Haiyan Liu,Shan Liu,Baoying Fei

Science advances 11:eadu4270 PubMed40749060

2025

Neuronal Reg3β/macrophage TNF-α-mediated positive feedback signaling contributes to pain chronicity in a rat model of CRPS-I.

Applications

Unspecified application

Species

Unspecified reactive species

Huimin Nie,Boyu Liu,Chengyu Yin,Zishan Dong,Yushuang Pan,Peiyi Li,Qimiao Hu,Jie Wang,Yan Tai,Xiaomei Shao,Guihua Tian,Chuan Wang,Jianqiao Fang,Boyi Liu

International journal of molecular sciences 26: PubMed40724911

2025

Biochemical Insights into the Effects of a Small Molecule Drug Candidate on Imatinib-Induced Cardiac Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Renáta Szabó,Denise Börzsei,András Nagy,Viktória Kiss,Zoltán Virág,Gyöngyi Kis,Nikoletta Almási,Szilvia Török,Médea Veszelka,Mária Bagyánszki,Nikolett Bódi,Bence Pál Barta,Patrícia Neuperger,Gabor J Szebeni,Csaba Varga

Journal of thoracic disease 17:4249-4263 PubMed40688308

2025

regulation of cardiac fibroblast proliferation via the TGF-β/Smad pathway in an model of atrial fibrillation.

Applications

Unspecified application

Species

Unspecified reactive species

Yimeng Zhou,Lianzhi Zhang,Wenjia Zhang,Qunlin Gong,Zhong Zhang,Nan Xu,Ashley R Wilson-Smith,Nannan Chen

Nature neuroscience 28:1688-1703 PubMed40659845

2025

Response of spatially defined microglia states with distinct chromatin accessibility in a mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Alberto Ardura-Fabregat,Lance Fredrick Pahutan Bosch,Emile Wogram,Omar Mossad,Roman Sankowski,Philipp Aktories,Lina Kieger,James Cook,Dilara Hasavci,Hatice Ulupinar,Daniel Brock,Fang Wang,Nicola Iovino,Samuel Wald,Sebastian Preissl,Bahtiyar Yilmaz,Daniel Schnepf,Andrew J Macpherson,Thomas Blank,Katrin Kierdorf,Marco Prinz

International journal of molecular sciences 26: PubMed40649891

2025

Targeting PAD4: A Promising Strategy to Combat β-Cell Loss in Type 1 Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Hsu Lin Kang,András Szász,Zsuzsanna Valkusz,Tamás Várkonyi,Anikó Pósa,Krisztina Kupai

Brain communications 7:fcaf224 PubMed40574971

2025

Glycine-to-aspartic acid mutation at codon 51 in disrupts the synaptic localisation of α-synuclein and enhances its propensity for synucleinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Stephen West,Ammar Natalwala,Karamjit Singh Dolt,Douglas J Lamont,Melanie McMillan,Kelvin Luk,Tomoji Mashimo,Tilo Kunath

Nature communications 16:5209 PubMed40473625

2025

Lysosomal TMEM165 controls cellular ion homeostasis and survival by mediating lysosomal Ca import and H efflux.

Applications

Unspecified application

Species

Unspecified reactive species

Ran Chen,Bin Liu,Dawid Jaślan,Lucija Kucej,Veronika Kudrina,Belinda Warnke,Yvonne E Klingl,Arnas Petrauskas,Sandra Prat Castro,Kenji Maeda,Christian Grimm,Marja Jäättelä

Cell reports. Medicine 6:102135 PubMed40398389

2025

MPXV infection activates cGAS-STING signaling and IFN-I treatment reduces pathogenicity of mpox in CAST/EiJ mice and rhesus macaques.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Zhu,Qi Liu,Yongzhi Hou,Baoying Huang,Dong Zhang,Zhe Cong,Jianrong Ma,Na Li,Jiahan Lu,Jingjing Zhang,Lingyan Zhang,Ting Chen,Qiang Wei,Jiangning Liu,Wenjie Tan,Jing Xue

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-beta Actin 抗体 [mAbcam 8226] - Loading Control

HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control

Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (AB20272)

Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (AB20272)

Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (AB20272)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

軽鎖のタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

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Primary Antibodies

Primary Antibodies

Primary Antibodies

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Primary Antibodies

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Reagents, Controls & Accessories

Reagents, Controls & Accessories

Reagents, Controls & Accessories

Sample Prep & Detection Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製度

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (377)

Abcam product promise