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Anti-HRP 抗体 (ab2116)

Submit a review Q&A (2)References (2)

Key features and details

  • Sheep polyclonal to HRP
  • Suitable for: ELISA, Conjugation, IP, WB
  • Reacts with: Horseradish
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-HRP antibody
    HRP 一次抗体 製品一覧

  • 製品の詳細

    Sheep polyclonal to HRP

  • 由来種

    Sheep

  • アプリケーション

    適用あり: ELISA, Conjugation, IP, WBmore details

  • 種交差性

    交差種: Horseradish

  • 免疫原

    Full length native protein (purified) corresponding to HRP. Horseradish peroxidase

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Lyophilized:Restore with 100ul deionized water

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
  • Concentration information loading...

  • 精製度

    Ion Exchange Chromatography

  • 特記事項（精製）

    Anti-PEROXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab2116の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
1/2000 - 1/10000.
Conjugation
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
1/500 - 1/3000.
特記事項
ELISA
1/2000 - 1/10000.
Conjugation
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
1/500 - 1/3000.

ターゲット情報

  • 関連性

    Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.

  • 細胞内局在

    Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles.

  • 参照データベース

    • 別名

      • Horseradish Peroxidase antibody
      • HPRC1 antibody
      • HRP antibody
      • Peroxidase antibody
      • Peroxidase C1A antibody
      • PRXC1A antibody
      see all

    参考文献 (2)

    ab2116 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2116 は 2 報の論文で使用されています。

    • Evers L  et al. Identification of Dysregulated microRNAs in Glioblastoma Stem-like Cells. Brain Sci 13:N/A (2023). PubMed: 36831894
    • Li YF  et al. Targeting MRP4 expression by anti-androgen treatment reverses MRP4-mediated docetaxel resistance in castration-resistant prostate cancer. Oncol Lett 14:1748-1756 (2017). PubMed: 28789405

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    is this antiserum suitable for inhibiting peroxidase activity?

    Read More
    Answer

    The antibody has not been tested for inhibition of peroxidase. We have introduced a new scheme to allow researchers to try an antibody in an unknown application 'risk free'. If you want to place an order with our reference LD/NS.02.08.01 we can include the antibody in this scheme (No#2 below applies in this case). 1.) Abcam will refund the product if it does not work in the application and species as stated on the datasheet. 2.) If it is used in an application or on a species that is not listed on the datasheet then Abcam will refund or replace the product for another of equal value as long as Abcam receives the negative data for publishing on the datasheets to help other researchers.

    Read More

    Question

    I have tumours grown using transfectants stably expressing the HRP enzyme and would like to stain using immunohistochemistry. Are any of your horseradish peroxidase antibodies suitable for IHC in either frozen or paraffin embeded sections, and would they also pick up other peroxidases present in the tissue such as myeloperoxidase?

    Read More
    Answer

    We do not have any reports of any of our HRP antibodies being used as a primary antibody with frozen or paraffin embedded sections. In addition, cross-reactivity with endogenous tissue peroxidases has not been tested.

    Read More

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