Anti-HRP 抗体 (ab181658)
Key features and details
- Goat polyclonal to HRP
- Suitable for: WB
- Reacts with: Horseradish
- Isotype: IgG
製品の概要
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製品名
Anti-HRP antibody
HRP 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to HRP -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Horseradish -
免疫原
Full length native protein (purified) corresponding to HRP. (Horseradish).
Database link: P00433 -
ポジティブ･コントロール
- Horseradish peroxidase
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.27% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading... -
精製度
IgG fraction -
特記事項（精製）
ab181658 was purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Related Products
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- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
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- 450 nm Stop Solution for TMB Substrate (ab171529)
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- Immunoassay Blocking Buffer (ab171534)
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アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181658の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 39 kDa.
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| 特記事項 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 39 kDa. |
ターゲット情報
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関連性
Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue. -
細胞内局在
Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles. -
参照データベース
- SwissProt: P00433 Horseradish
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別名
- Horseradish Peroxidase antibody
- HPRC1 antibody
- HRP antibody
see all
画像
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Western blot - Anti-HRP antibody (ab181658)All lanes : Anti-HRP antibody (ab181658) at 1/5000 dilution
Lane 1 : Horseradish peroxidase (reducing conditions) at 1 µg
Lane 2 : Horseradish peroxidase (reducing conditions) at 0.25 µg
Lane 3 : Horseradish peroxidase (non-reducing conditions) at 1 µg
Lane 4 : Horseradish peroxidase (non-reducing conditions) at 0.25 µg
Secondary
All lanes : Dylight 649 conjugated Donkey anti Goat at 1/10000 dilution
Predicted band size: 39 kDaReduced samples of purified Horseradish peroxidase contained 4% BME and were boiled for 5 minutes.
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Western blot - Anti-HRP antibody (ab181658)Anti-HRP antibody (ab181658) at 1/1000 dilution + Peroxidase at 0.05 µg
Secondary
DyLight™ 649 goat secondary at 1/20000 dilution
Predicted band size: 39 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (23)
ab181658 は 23 報の論文で使用されています。
- Liang X et al. Dickkopf‑3 and β‑catenin play opposite roles in the Wnt/β‑catenin pathway during the abnormal subchondral bone formation of human knee osteoarthritis. Int J Mol Med 49:N/A (2022). PubMed: 35137918
- Long J et al. miR-500a-3p is a Potential Prognostic Biomarker in Hepatocellular Carcinoma. Int J Gen Med 15:1891-1899 (2022). PubMed: 35221718
- Tan Y et al. Circular RNA ZFR promotes cell cycle arrest and apoptosis of colorectal cancer cells via the miR-147a/CACUL1 axis. J Gastrointest Oncol 13:1793-1804 (2022). PubMed: 36092343
- Sun M et al. Ginkgolide B alleviates the inflammatory response and attenuates the activation of LPS-induced BV2 cells in vitro and in vivo. Exp Ther Med 21:586 (2021). PubMed: 33850558
- Liu W & Liu X LINC00641 Inhibits the Development of Cutaneous Squamous Cell Carcinoma By Downregulating miR-424 in A431 Cells. Cancer Biother Radiopharm N/A:N/A (2021). PubMed: 33885353