Anti-HRP 抗体 [2H11] (ab10183)
Key features and details
- Mouse monoclonal [2H11] to HRP
- Suitable for: ELISA, IHC-Fr, IHC-P
- Reacts with: Horseradish
- Isotype: IgG2b
製品の概要
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製品名
Anti-HRP antibody [2H11]
HRP 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2H11] to HRP -
由来種
Mouse -
特異性
Horseradish peroxidase isoforms with pI from 3.5 to 9.5. ab10183 does not influence enzymatic activity upon immune complex formation. -
アプリケーション
適用あり: ELISA, IHC-Fr, IHC-Pmore details -
種交差性
交差種: Horseradish -
免疫原
Full length native protein (purified) corresponding to HRP.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis. -
ポリ/モノ
モノクローナル -
クローン名
2H11 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10183の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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関連性
Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue. -
細胞内局在
Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles. -
参照データベース
- SwissProt: P00433 Horseradish
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別名
- Horseradish Peroxidase antibody
- HPRC1 antibody
- HRP antibody
see all
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab10183 は 10 報の論文で使用されています。
- Du J Synergistic effect of Bruton's tyrosine kinase and TNF-α in the regulation of rheumatoid arthritis and underlying mechanisms. Exp Ther Med 23:141 (2022). PubMed: 35069822
- Zhang B et al. Up-regulation of miR-204 inhibits proliferation, invasion and apoptosis of gallbladder cancer cells by targeting Notch2. Aging (Albany NY) 13:2941-2958 (2021). PubMed: 33460397
- Wang Z et al. Long noncoding RNA PTENP1 affects the recovery of spinal cord injury by regulating the expression of miR-19b and miR-21. J Cell Physiol 235:3634-3645 (2020). PubMed: 31583718
- Sharafeldin M et al. Influence of antibody immobilization strategy on carbon electrode immunoarrays. Analyst 144:5108-5116 (2019). PubMed: 31373337
- Liu B et al. Effect of microRNA-27b on cisplatin chemotherapy sensitivity of oral squamous cell carcinoma via FZD7 signaling pathway. Oncol Lett 18:667-673 (2019). PubMed: 31289540
- Lin TY et al. Mapping chromatic pathways in the Drosophila visual system. J Comp Neurol 524:213-27 (2016). PubMed: 26179639
- Miyazaki T et al. A gustatory second-order neuron that connects sucrose-sensitive primary neurons and a distinct region of the gnathal ganglion in the Drosophila brain. J Neurogenet 29:144-55 (2015). PubMed: 26004543
- Shazeeb MS et al. Targeted signal-amplifying enzymes enhance MRI of EGFR expression in an orthotopic model of human glioma. Cancer Res 71:2230-9 (2011). IHC-Fr . PubMed: 21245103
- Terashima H et al. A sensitive short-term evaluation of antifibrotic effects using newly established type I collagen reporter transgenic rats. Am J Physiol Renal Physiol 299:F792-801 (2010). IHC-Fr . PubMed: 20660018
- He DF et al. Morpho-Physiologic Characteristics of Dorsal Subicular Network in Mice after Pilocarpine-Induced Status Epilepticus. Brain Pathol : (2009). IHC . PubMed: 19298597