AB202004

HRP Anti-6X His tag® 抗体 [4D11]

Name: HRP Anti-6X His tag® 抗体 [4D11] (ab202004) | Abcam
Brand: Abcam Limited
SKU: AB202004
Price: 84000 JPY
Availability: InStock

HRP Anti-6X His tag® antibody [4D11]

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(2 Publications)

Anti-6X His tag® antibody [4D11] conjugated to HRP - suitable for Western blot samples. Clone [4D11] is a mouse monoclonal antibody cited in 10 publications.

別名を表示する

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6x his, 6x-HIS

1 Images

Lab

Western blot - HRP Anti-6X His tag® antibody [4D11] (AB202004)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab202004 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-6X His tag® antibody [4D11] (ab202004) at 1/5000 dilution

All lanes:

Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (<a href='/products/cell-lysates/e-coli-positive-control-escherichia-coli-whole-cell-lysate-ab5395'>ab5395</a>) at 0.1 µg

true

Exposure time: 3min

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

4D11

アイソタイプ

IgG2a

軽鎖のタイプ

kappa

標識

HRP

励起波長/蛍光波長

キャリアフリー

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

エピトープ

This antibody was shown to recognize an epitope (HHHHHHGS) encoded by the polylinker of several expression vectors (including pDS:RBSII, pQE-30, and pQE-50).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }

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製品の詳細

HIS-TAG® is a trademark of EMD Biosciences, Inc.

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出荷温度及び保存条件

製品の状態

Liquid

精製度

IgG fraction

バッファー組成

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Store in the dark

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information 6X His tag®

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Microbiology spectrum :e0204324 PubMed39436135

2024

Development and optimization of a double antigen sandwich ELISA detecting for Senecavirus A antibodies based on VP2 protein.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Chen,Zhengwang Shi,Juncong Luo,Caixia Jia,Xiaoyang Zhang,Juanjuan Wei,Shuaipeng Li,Yuqian Zhu,Tao Xi,Jing Zhou,Yindi He,Xintai Shi,Huanchen Liao,Hong Tian,Haixue Zheng

Heliyon 10:e30526 PubMed38737268

2024

Changes in transcriptomic landscape with macronutrients intake switch are independent from O-GlcNAcylation levels in heart throughout postnatal development in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Antoine Persello,Thomas Dupas,Amandine Vergnaud,Angélique Blangy-Letheule,Virginie Aillerie,Angélique Erraud,Yannick Guilloux,Manon Denis,Benjamin Lauzier

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-6X His tag® 抗体 [4D11]

HRP Anti-6X His tag® antibody [4D11]

Western blot - HRP Anti-6X His tag® antibody [4D11] (AB202004)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

軽鎖のタイプ

標識

励起波長/蛍光波長

キャリアフリー

アプリケーション

免疫原

エピトープ

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製度

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

Abcam product promise