Anti-Hrk 抗体 (ab45419)
Key features and details
- Rabbit polyclonal to Hrk
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Hrk antibody -
製品の詳細
Rabbit polyclonal to Hrk -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide of 15 amino acids in length from near the center of human Hrk
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ポジティブ･コントロール
- Mouse pancreas tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab45419の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| WB | (1) |
Use a concentration of 2.5 - 5 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 10 kDa).
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| 特記事項 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
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WB
Use a concentration of 2.5 - 5 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 10 kDa). |
ターゲット情報
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機能
Promotes apoptosis. -
ドメイン
The BH3 motif is required for the induction of cell death. -
細胞内局在
Membrane. Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 8739 Human
- Entrez Gene: 12123 Mouse
- Omim: 603447 Human
- SwissProt: O00198 Human
- SwissProt: P62816 Mouse
- Unigene: 87247 Human
- Unigene: 384468 Mouse
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別名
- Activator of apoptosis harakiri antibody
- Activator of apoptosis Hrk antibody
- BCL2 interacting protein antibody
see all
画像
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Western blot - Anti-Hrk antibody (ab45419)Lane 1 : Anti-Hrk antibody (ab45419) at 2.5 µg/ml
Lane 2 : Anti-Hrk antibody (ab45419) at 5 µg/ml
All lanes : Mouse pancreas tissue lysate
Predicted band size: 10 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab45419 は 5 報の論文で使用されています。
- Dunn S et al. AKT-mTORC1 reactivation is the dominant resistance driver for PI3Kβ/AKT inhibitors in PTEN-null breast cancer and can be overcome by combining with Mcl-1 inhibitors. Oncogene 41:5046-5060 (2022). PubMed: 36241868
- Liang R et al. The Stress-Inducible BCL2A1 Is Required for Ovarian Cancer Metastatic Progression in the Peritoneal Microenvironment. Cancers (Basel) 13:N/A (2021). PubMed: 34572804
- Duval N et al. The Bcl-2 Homology-3 Domain (BH3)-Only Proteins, Bid, DP5/Hrk, and BNip3L, Are Upregulated in Reactive Astrocytes of End-Stage Mutant SOD1 Mouse Spinal Cord. Front Cell Neurosci 12:15 (2018). WB ; Mouse . PubMed: 29440992
- Li D et al. Armadillo repeat containing 12 promotes neuroblastoma progression through interaction with retinoblastoma binding protein 4. Nat Commun 9:2829 (2018). PubMed: 30026490
- Wang NN et al. Molecular targeting of the oncoprotein PLK1 in pediatric acute myeloid leukemia: RO3280, a novel PLK1 inhibitor, induces apoptosis in leukemia cells. Int J Mol Sci 16:1266-92 (2015). PubMed: 25574601