Anti-HP1 alpha 抗体 (ab77256)

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;

Questions - ChIP

Sample type, species
Sample preparation (X-ChIP/N-ChIP, cross-linking conditions, DNA fragment size)
Immunoprecipitation conditions (buffer, amount of detergent, primary antibody manufacturer/dilution, incubation time, matrix for isolating antibody complex)
Wash steps
DNA purification (decrosslinking conditions, DNA purification method)
DNA detection method (regions amplified in PCR, microarray, direct sequencing)
Controls used (positive/negative antibodies, positive/negative control regions, no template control in PCR)
Lot number/order number/date of purchase



WB

Sample preparation (cell type, species, lysis buffer, protease inhibitors, phosphatase inhibitors)
Amount of protein loaded
Electrophoresis and transfer conditions
Blocking conditions
Primary antibody conditions (diluent/dilution, incubation time/temperature)
Secondary antibody conditions (diluent/dilution, incubation time/temperature)
Wash steps and buffer
Detection method
Controls used (sample, alternative primary ab, no primary)



Thank you very much for your cooperation. I will look forward to hearing from you soon.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxxx. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.