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Anti-HOXD11 抗体 [5G4] (ab55255)

Reviews (1) Submit a question References (1)
Western blot - Anti-HOXD11 antibody [5G4] (ab55255)
  • Immunocytochemistry/ Immunofluorescence - Anti-HOXD11 antibody [5G4] (ab55255)

Key features and details

  • Mouse monoclonal [5G4] to HOXD11
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-HOXD11 antibody [5G4]
    HOXD11 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [5G4] to HOXD11

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, ICC/IFmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment: MNDFDECGQS AASMYLPGCA YYVAPSDFAS KPSFLSQPSS CQMTFPYSSN LAPHVQPVRE VAFRDYGLER AKWPYR, corresponding to amino acids 1-77 of Human HOXD11

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 27 June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • バッファー

    pH: 7.4
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • 特記事項（精製）

    Purified from TCS.

  • ポリ/モノ

    モノクローナル

  • クローン名

    5G4

  • アイソタイプ

    IgG2a

  • 軽鎖の種類

    kappa

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab55255の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 36 kDa.
ICC/IF
Use a concentration of 10 µg/ml.
特記事項
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 36 kDa.
ICC/IF
Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能

    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.

  • 配列類似性

    Belongs to the Abd-B homeobox family.
    Contains 1 homeobox DNA-binding domain.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Homeo box 4F antibody
      • Homeo box D11 antibody
      • Homeobox D11 antibody
      • Homeobox protein Hox-4F antibody
      • Homeobox protein Hox-D11 antibody
      • Hox-4F antibody
      • HOX4 antibody
      • HOX4F antibody
      • HOXD11 antibody
      • HXD11_HUMAN antibody
      see all

    画像

    • Western blot - Anti-HOXD11 antibody [5G4] (ab55255)
      Western blot - Anti-HOXD11 antibody [5G4] (ab55255)

      HOXD11 antibody (ab55255) at 1ug/lane + K-562 cell lysate at 25ug/lane.

      This image was generated using the ascites version of the product.

    • Immunocytochemistry/ Immunofluorescence - Anti-HOXD11 antibody [5G4] (ab55255)
      Immunocytochemistry/ Immunofluorescence - Anti-HOXD11 antibody [5G4] (ab55255)

      ab55255 at 10 ug/ml staining HOXD11 in human cells by Immunocytochemistry/ Immunofluorescence.

      This image was generated using the ascites version of the product.

    参考文献 (1)

    ab55255 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab55255 は 1 報の論文で使用されています。

    • Tsujimoto H  et al. A Modular Differentiation System Maps Multiple Human Kidney Lineages from Pluripotent Stem Cells. Cell Rep 31:107476 (2020). PubMed: 32268094

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Western blot abreview for Anti-HOXD11 antibody

     Inconclusive
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - nuclear (embryonic limb tissue)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    40 µg
    Specification
    embryonic limb tissue
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3µg/mL · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Racheal Paul

    Verified customer

    投稿 Jan 26 2021

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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