AB249014

Anti-HoxC6 抗体 [EPR7922(2)] - BSA and Azide free

Anti-HoxC6 antibody [EPR7922(2)] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal HoxC6 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

別名を表示する

HOX3C, HOXC6, Homeobox protein Hox-C6, Homeobox protein CP25, Homeobox protein HHO.C8, Homeobox protein Hox-3C

2 Images

Flow Cytometry (Intracellular) - Anti-HoxC6 antibody [EPR7922(2)] - BSA and Azide free (AB249014)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-HoxC6 antibody [EPR7922(2)] - BSA and Azide free (AB249014)

This data was developed using ab151575, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized MCF7 cells labeling HoxC6 with ab151575 at 1/100 dilution (red), compared to a Rabbit IgG negative control antibody (green).

Unknown

Western blot - Anti-HoxC6 antibody [EPR7922(2)] - BSA and Azide free (AB249014)

This data was developed using ab151575, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HoxC6 antibody [EPR7922(2)] (<a href='/products/primary-antibodies/hoxc6-antibody-epr79222-ab151575'>ab151575</a>) at 1/1000 dilution

Lane 1:

HUVEC cell lysate at 10 µg

Lane 2:

PC3 cell lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 26 kDa

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Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR7922(2)

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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製品の詳細

ab249014 is the carrier-free version of ab151575.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Do Not Freeze

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

HoxC6 also known as Homeobox C6 is a transcription factor that guides the formation of body structure during early development. The protein weighs approximately 25 kDa and plays an important role in sequencing and pattern formation in the embryonic development stages. It is expressed mainly in the central nervous system and developing tissues but also shows functionality in adult tissues. The activity of HoxC6 affects cellular differentiation and proliferation by regulating specific gene expression in these regions.

Biological function summary

The protein binds to DNA at specific sequences to activate or repress target gene transcription. This can affect cell fate and organ morphogenesis. HoxC6 associates with other Hox proteins and cofactors to form complexes that enhance or modify its regulatory functions. These interactions with cofactor proteins further strengthen its impact on developmental processes ensuring accurate tissue and organ formation.

Pathways

HoxC6 functions within pivotal developmental pathways such as the Hox gene regulatory network. This network synchronizes with signaling pathways like the Wnt and Notch controlling cell proliferation and differentiation. The collaboration with these pathways allows HoxC6 to manage gene cascades that maintain the correct body patterning. HoxB5 a related protein in the same pathway often interacts or shares similar roles coordinating to maintain orderly tissue patterning.

Malfunction or mis-expression of HoxC6 links to certain cancers including leukemia and breast cancer. The protein's deregulation can lead to abnormal cell growth and differentiation. It works alongside proteins like Myc which also plays a central role in cancer to influence oncogenesis. Studies continue to explore HoxC6's involvement in these diseases considering its role in normal cellular development and potential therapeutic targeting.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.

See full target information HOXC6

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HoxC6 抗体 [EPR7922(2)] - BSA and Azide free

Anti-HoxC6 antibody [EPR7922(2)] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-HoxC6 antibody [EPR7922(2)] - BSA and Azide free (AB249014)

Western blot - Anti-HoxC6 antibody [EPR7922(2)] - BSA and Azide free (AB249014)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

Abcam product promise