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リコンビナントRabMAb

Anti-HoxC6 抗体 [EPR7922(2)] (ab151575)

Submit a review Q&A (1)References (7)
Western blot - Anti-HoxC6 antibody [EPR7922(2)] (ab151575)
  • Flow Cytometry (Intracellular) - Anti-HoxC6 antibody [EPR7922(2)] (ab151575)
  • Anti-HoxC6 antibody [EPR7922(2)] (ab151575)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7922(2)] to HoxC6
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-HoxC6 antibody [EPR7922(2)]
    HoxC6 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR7922(2)] to HoxC6

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WBmore details
    適用なし: ICC/IF,IHC-P or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human HoxC6 aa 1-100. The exact sequence is proprietary.

  • ポジティブ･コントロール

    • HUVEC, PC3, MCF7 and Jurkat whole cell lysate (ab7899); MCF7 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at -20ºC.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR7922(2)

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab151575の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
特記事項
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
追加情報
Is unsuitable for ICC/IF,IHC-P or IP.

ターゲット情報

  • 機能

    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.

  • 配列類似性

    Belongs to the Antp homeobox family.
    Contains 1 homeobox DNA-binding domain.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CP25 antibody
      • HHO.C8 antibody
      • Homeo box 3C antibody
      • Homeo box C6 antibody
      • Homeo box C8 protein antibody
      • Homeobox C6 antibody
      • Homeobox protein CP25 antibody
      • Homeobox protein HHO.C8 antibody
      • Homeobox protein Hox-3C antibody
      • Homeobox protein Hox-C6 antibody
      • HOX3 antibody
      • HOX3C antibody
      • Hoxc6 antibody
      • HXC6_HUMAN antibody
      see all

    画像

    • Western blot - Anti-HoxC6 antibody [EPR7922(2)] (ab151575)
      Western blot - Anti-HoxC6 antibody [EPR7922(2)] (ab151575)
      All lanes : Anti-HoxC6 antibody [EPR7922(2)] (ab151575) at 1/1000 dilution

      Lane 1 : HUVEC cell lysate
      Lane 2 : PC3 cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 26 kDa

    • Flow Cytometry (Intracellular) - Anti-HoxC6 antibody [EPR7922(2)] (ab151575)
      Flow Cytometry (Intracellular) - Anti-HoxC6 antibody [EPR7922(2)] (ab151575)

      Intracellular flow cytometric analysis of permeabilized MCF7 cells labeling HoxC6 with ab151575 at 1/100 dilution (red), compared to a Rabbit IgG negative control antibody (green).

       

    • Anti-HoxC6 antibody [EPR7922(2)] (ab151575)
      Anti-HoxC6 antibody [EPR7922(2)] (ab151575)

    参考文献 (7)

    ab151575 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab151575 は 7 報の論文で使用されています。

    • Huang H  et al. HOXC6 impacts epithelial-mesenchymal transition and the immune microenvironment through gene transcription in gliomas. Cancer Cell Int 22:170 (2022). PubMed: 35488304
    • Long X  et al. HomeoboxC6 affects the apoptosis of human vascular endothelial cells and is involved in atherosclerosis. J Cell Physiol 236:1913-1925 (2021). PubMed: 32740941
    • Xu J  et al. LncRNA BBOX1-AS1 upregulates HOXC6 expression through miR-361-3p and HuR to drive cervical cancer progression. Cell Prolif 53:e12823 (2020). PubMed: 32515533
    • Shen LY  et al. Targeting HOX/PBX dimer formation as a potential therapeutic option in esophageal squamous cell carcinoma. Cancer Sci 110:1735-1745 (2019). PubMed: 30844117
    • Yan TF  et al. Knockdown of HOXC6 inhibits glioma cell proliferation and induces cell cycle arrest by targeting WIF-1 in vitro and vivo. Pathol Res Pract 214:1818-1824 (2018). PubMed: 30228024
    • Zhang F  et al. HOXC6 gene silencing inhibits epithelial-mesenchymal transition and cell viability through the TGF-ß/smad signaling pathway in cervical carcinoma cells. Cancer Cell Int 18:204 (2018). PubMed: 30559605
    • Shen LY  et al. Increased HOXC6 expression predicts chemotherapy sensitivity in patients with esophageal squamous cell carcinoma. Oncol Lett 14:4835-4840 (2017). PubMed: 29085488

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    I'm looking for an antibody that i can use in an EMSA supershift assay. Computational models predict that various HOX proteins (including HOXA1, 2, 3, 6 and 7, and HOXB 5, 6, and 7, and HOXC 6 all might bind in that region. Do you have an antibody that could detect a variety of HOX proteins?

    Read More
    Answer

    We currently have over forty products against these HOX targets. These product will be specific to the HOX protein which they are raised against, whether A1, A2, A3 etc. We currently do not carry an anti-HOX antibody which will recognize a number of HOX proteins. While we do not test for and cannot guarantee efficacy of our antibodies in EMSA super shift, I have reviewed the catalog for products which would be most suitable for this assay. Generally we would want to look for monoclonal antibodies which have been show to recognize the protein in a native form. For targets which do not have monoclonal antibodies available, I have chosen the best available alternative for you. This list is included in the spreadsheet which I have attached to this email.

    Read More

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