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リコンビナントRabMAb

Anti-Hormone sensitive lipase/HSL (phospho S855) 抗体 [EPR2330] (ab76492)

Submit a review Q&A (1)References (1)
Western blot - Anti-Hormone sensitive lipase/HSL (phospho S855) antibody [EPR2330] (ab76492)
  • Anti-Hormone sensitive lipase/HSL (phospho S855) antibody [EPR2330] (ab76492)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2330] to Hormone sensitive lipase/HSL (phospho S855)
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Hormone sensitive lipase/HSL (phospho S855) antibody [EPR2330]
    Hormone sensitive lipase/HSL 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR2330] to Hormone sensitive lipase/HSL (phospho S855)

  • 由来種

    Rabbit

  • 特異性

    Detects HSL/LIPE only when phosphorylated on Serine 855

  • アプリケーション

    適用あり: WBmore details
    適用なし: ICC/IF,IHC-P or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human skeletal muscle lysate

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab76492の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/5000. Predicted molecular weight: 117 , 83 kDa.

Predicted molecular weight: 117 kDa (isoform 1) or 83 kDa (isoform 2).
特記事項
WB
1/1000 - 1/5000. Predicted molecular weight: 117 , 83 kDa.

Predicted molecular weight: 117 kDa (isoform 1) or 83 kDa (isoform 2).
追加情報
Is unsuitable for ICC/IF,IHC-P or IP.

ターゲット情報

  • 機能

    In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.

  • パスウェイ

    Glycerolipid metabolism; triacylglycerol degradation.

  • 配列類似性

    Belongs to the 'GDXG' lipolytic enzyme family.

  • 細胞内局在

    Cell membrane. Membrane > caveola. Cytoplasm > cytosol. Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Hormone sensitive lipase antibody
    • Hormone sensitive lipase testicular isoform antibody
    • Hormone-sensitive lipase antibody
    • HSL antibody
    • LHS antibody
    • Lipase hormone sensitive antibody
    • LIPE antibody
    • LIPS_HUMAN antibody
    see all

画像

  • Western blot - Anti-Hormone sensitive lipase/HSL (phospho S855) antibody [EPR2330] (ab76492)
    Western blot - Anti-Hormone sensitive lipase/HSL (phospho S855) antibody [EPR2330] (ab76492)
    All lanes : Anti-Hormone sensitive lipase/HSL (phospho S855) antibody [EPR2330] (ab76492) at 1/5000 dilution

    Lane 1 : Human skeletal muscle lysate, untreated
    Lane 2 : Human skeletal muscle lysate, treated with LP

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/1000 dilution

    Predicted band size: 117 , 83 kDa
    Observed band size: 83 kDa why is the actual band size different from the predicted?

  • Anti-Hormone sensitive lipase/HSL (phospho S855) antibody [EPR2330] (ab76492)
    Anti-Hormone sensitive lipase/HSL (phospho S855) antibody [EPR2330] (ab76492)

参考文献 (1)

ab76492 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab76492 は 1 報の論文で使用されています。

  • Chadwick ML  et al. Combined mTOR and MEK inhibition is an effective therapy in a novel mouse model for angiosarcoma. Oncotarget 9:24750-24765 (2018). PubMed: 29872503

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

Question

Does this antibody recognzing the long isoform or the short isoform? Also, will it work with mouse? Does it recognize the analogous S565 in the mouse sequence?

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Answer

Thank you for your inquiry.

The Serine 855 of human HSL is equivalent to Serine 559 of mouse HSL, and this would recognize the Serine 559 of mouse HSL. The orthologous residue for isoform 2 of the rat HSL would be Serine 565, and this antibody should recognize it as well, although the rat is not recommended based on our WB analysis.


I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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