Anti-Hormone sensitive lipase/HSL 抗体 (ab45422)
Key features and details
- Rabbit polyclonal to Hormone sensitive lipase/HSL
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Hormone sensitive lipase/HSL antibody
Hormone sensitive lipase/HSL 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Hormone sensitive lipase/HSL -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This antibody is an immunoaffinity purified IgG -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab45422の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
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| ICC/IF | (1) |
Use a concentration of 20 µg/ml.
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| WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 117 kDa).
|
| 特記事項 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
|
ICC/IF
Use a concentration of 20 µg/ml. |
|
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 117 kDa). |
ターゲット情報
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機能
In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. -
パスウェイ
Glycerolipid metabolism; triacylglycerol degradation. -
配列類似性
Belongs to the 'GDXG' lipolytic enzyme family. -
細胞内局在
Cell membrane. Membrane > caveola. Cytoplasm > cytosol. Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation. - Information by UniProt
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参照データベース
- Entrez Gene: 3991 Human
- Entrez Gene: 16890 Mouse
- Entrez Gene: 25330 Rat
- Omim: 151750 Human
- SwissProt: Q05469 Human
- SwissProt: Q6NSL7 Human
- SwissProt: P54310 Mouse
- SwissProt: P15304 Rat
see all -
別名
- Hormone sensitive lipase antibody
- Hormone sensitive lipase testicular isoform antibody
- Hormone-sensitive lipase antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Hormone sensitive lipase/HSL antibody (ab45422)This image is courtesy of an Abreview submitted by Kirk McManus
ab45422 staining Hormone sensitive lipase/HSL in the HeLa cell line from Human cervical cancer by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/200 in PBS) for 16 hour at 22°C. ab150081 an Alexa Fluor®488-conjugated Goat anti-rabbit IgG H&L (1/200) polyclonal was used as the secondary antibody. Nuclei were stained with DAPI.MeOH-fixed samples were also evaluated and failed to produce the expected staining pattern.
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Western blot - Anti-Hormone sensitive lipase/HSL antibody (ab45422)Lane 1 : Anti-Hormone sensitive lipase/HSL antibody (ab45422) at 0.5 µg/ml
Lane 2 : Anti-Hormone sensitive lipase/HSL antibody (ab45422) at 1 µg/ml
All lanes : Human lymph node tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 117 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa, 86 kDa, 88 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-Hormone sensitive lipase/HSL antibody (ab45422)Immunofluorescence of Lipe in Human Lymph Node cells using ab45422 at 20 ug/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (48)
ab45422 は 48 報の論文で使用されています。
- Zhong R et al. Nomegestrol acetate ameliorated adipose atrophy in a rat model of cisplatin‑induced cachexia. Exp Ther Med 25:24 (2023). PubMed: 36561625
- Kim JW et al. Novel Plant Extract Ameliorates Metabolic Disorder through Activation of Brown Adipose Tissue in High-Fat Diet-Induced Obese Mice. Int J Mol Sci 23:N/A (2022). PubMed: 36012561
- Yang Z et al. Polyphyllin I improves myocardial damage in coronary artery disease via modulating lipid metabolism and myocardial apoptosis. J Biochem Mol Toxicol 36:e23219 (2022). PubMed: 36120828
- Park WY et al. Anmyungambi Decoction Ameliorates Obesity through Activation of Non-Shivering Thermogenesis in Brown and White Adipose Tissues. Antioxidants (Basel) 12:N/A (2022). PubMed: 36670911
- Yang X et al. Targeting adipocytic discoidin domain receptor 2 impedes fat gain while increasing bone mass. Cell Death Differ 29:737-749 (2022). PubMed: 34645939