Anti-HOOK2 抗体 (ab154109)
Key features and details
- Rabbit polyclonal to HOOK2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-HOOK2 antibody
HOOK2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HOOK2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow
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免疫原
Recombinant fragment, corresponding to a region within amino acids 197-380 of Human HOOK2 (UniProt ID: Q96ED9).
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ポジティブ･コントロール
- WB: HepG2, HCT116 and mouse liver whole cell lysates. IHC-P: Human MCF7 xenograft; Mouse duodenum and testis tissue. ICC/IF: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154109の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 83 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 83 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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関連性
HOOK2 is a member of the Hook protein family. Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. HOOK2 localizes to the centrosome through all phases of the cell cycle. Interference with HOOK2 function results in the loss of the radial organization of microtubules suggesting that HOOK2 contributes to the establishment and maintenance of centrosomal structure and function. HOOK2 may contribute to the establishment and maintenance of the pericentrosomal localization of aggresomes by promoting the microtubule-based delivery of protein aggregates to pericentriolar aggresomes. -
細胞内局在
Cytoplasmic. Note=Associates with discrete punctate structure that correspond to neither early or late endosomes, lysosomes, multivesicular bodies (MVBs), Golgi complex, endoplasmic reticulum, nor mitochondria. -
参照データベース
- Entrez Gene: 526516 Cow
- Entrez Gene: 29911 Human
- Entrez Gene: 170833 Mouse
- Entrez Gene: 304669 Rat
- Omim: 607824 Human
- SwissProt: Q96ED9 Human
- SwissProt: Q7TMK6 Mouse
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別名
- A630054I03Rik antibody
- FLJ26218 antibody
- h hook2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK2 antibody (ab154109)
Paraffin-embedded mouse duodenum tissue stained for HOOK2 using ab154109 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK2 antibody (ab154109)Paraffin-embedded mouse testis tissue stained for HOOK2 using ab154109 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-HOOK2 antibody (ab154109)PFA-fixed, Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HOOK2 (green) using ab154109 at 1/500 dilution in ICC/IF.
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Western blot - Anti-HOOK2 antibody (ab154109)Anti-HOOK2 antibody (ab154109) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg
Predicted band size: 83 kDa -
Western blot - Anti-HOOK2 antibody (ab154109)Anti-HOOK2 antibody (ab154109) at 1/1000 dilution + mouse liver whole cell lysate at 50 µg
Predicted band size: 83 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK2 antibody (ab154109)Immunofluorescent analysis of methanol-fixed HeLa cells labeling HOOK2 with ab154109 at 1/200 dilution.
The image in the lower panel was co-stained with Hoechst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK2 antibody (ab154109)Immunohistochemical analysis of paraffin embedded MCF7 xenograft tissue labeling HOOK2 with ab154109 at 1/100 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab154109 は 3 報の論文で使用されています。
- Kumari A et al. Phosphorylation and Pin1 binding to the LIC1 subunit selectively regulate mitotic dynein functions. J Cell Biol 220:N/A (2021). PubMed: 34709360
- Dwivedi D et al. Incorporating Motility in the Motor: Role of the Hook Protein Family in Regulating Dynein Motility. Biochemistry 58:1026-1031 (2019). PubMed: 30702276
- Dwivedi D et al. The dynein adaptor Hook2 plays essential roles in mitotic progression and cytokinesis. J Cell Biol 218:871-894 (2019). PubMed: 30674580