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リコンビナントRabMAb

Anti-HOOK1 抗体 [EPR10103(B)] (ab150397)

Submit a review Submit a question References (3)
Western blot - Anti-HOOK1 antibody [EPR10103(B)] (ab150397)
  • Anti-HOOK1 antibody [EPR10103(B)] (ab150397)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10103(B)] to HOOK1
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-HOOK1 antibody [EPR10103(B)]
    HOOK1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR10103(B)] to HOOK1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt,ICC/IF,IHC-P or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse

  • 免疫原

    Synthetic peptide within Human HOOK1 aa 700-800 (C terminal). The exact sequence is proprietary.

  • ポジティブ･コントロール

    • A673, MCF-7 and 293T cell lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab150397の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 85 kDa.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 85 kDa.
追加情報
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

ターゲット情報

  • 機能

    Required for spermatid differentiation. Probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton (By similarity). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex).

  • 配列類似性

    Belongs to the hook family.

  • 細胞内局在

    Cytoplasm > cytoskeleton. Cytoplasm. Localizes to the spermatid manchette during spermiogenesis but is not present in mature spermatozoa (By similarity). Localizes to punctate cytoplasmic foci which do not appear to overlap with early or late endosomes, the endoplasmic reticulum, the Golgi complex, multivesicular bodies (MVBs), lysosomes, or mitochondria. Often found in close association with microtubules.
  • Information by UniProt

  • 参照データベース

    • 別名

      • A930033L17Rik antibody
      • Abnormal spermatozoon head shape antibody
      • azh antibody
      • h-hook1 antibody
      • hHK1 antibody
      • HK1 antibody
      • HOOK 1 antibody
      • Hook homolog 1 (Drosophila) antibody
      • Hook1 antibody
      • HOOK1_HUMAN antibody
      • MGC10642 antibody
      • OTTHUMP00000010548 antibody
      • OTTMUSP00000008480 antibody
      • Protein Hook homolog 1 antibody
      • RP23-80B16.4 antibody
      see all

    画像

    • Western blot - Anti-HOOK1 antibody [EPR10103(B)] (ab150397)
      Western blot - Anti-HOOK1 antibody [EPR10103(B)] (ab150397)
      All lanes : Anti-HOOK1 antibody [EPR10103(B)] (ab150397) at 1/1000 dilution

      Lane 1 : A673 cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 85 kDa

    • Anti-HOOK1 antibody [EPR10103(B)] (ab150397)
      Anti-HOOK1 antibody [EPR10103(B)] (ab150397)

    参考文献 (3)

    ab150397 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab150397 は 3 報の論文で使用されています。

    • Olenick MA  et al. Dynein activator Hook1 is required for trafficking of BDNF-signaling endosomes in neurons. J Cell Biol 218:220-233 (2019). PubMed: 30373907
    • Do M  et al. Quantitative proteomic analysis of pancreatic cyst fluid proteins associated with malignancy in intraductal papillary mucinous neoplasms. Clin Proteomics 15:17 (2018). PubMed: 29713252
    • Olenick MA  et al. Hook Adaptors Induce Unidirectional Processive Motility by Enhancing the Dynein-Dynactin Interaction. J Biol Chem 291:18239-51 (2016). PubMed: 27365401

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