Name: Anti-HOOK1 antibody [EPR10102]
Brand: RabMAb
SKU: ab151756
Rating: 5 (1 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10102] to HOOK1
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human

Carrier Free

製品名

Anti-HOOK1 antibody [EPR10102]
HOOK1 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR10102] to HOOK1
由来種

Rabbit
アプリケーション

適用あり: WB, IHC-P, ICC/IFmore details
適用なし: Flow Cyt or IP
種交差性

交差種: Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- 293T or MCF-7 lysate, Human testis tissue or Caco-2 cells.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
バッファー

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR10102
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-HOOK1 antibody [EPR10102] - BSA and Azide free (ab249067)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab151756の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB	(1)	1/1000 - 1/10000. Predicted molecular weight: 85 kDa.
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use of an HRP/AP polymerized secondary antibody recommended.
ICC/IF		1/100 - 1/250.

特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 85 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use of an HRP/AP polymerized secondary antibody recommended.
ICC/IF 1/100 - 1/250.

追加情報

Is unsuitable for Flow Cyt or IP.

機能

Required for spermatid differentiation. Probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton (By similarity). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex).
配列類似性

Belongs to the hook family.
細胞内局在

Cytoplasm > cytoskeleton. Cytoplasm. Localizes to the spermatid manchette during spermiogenesis but is not present in mature spermatozoa (By similarity). Localizes to punctate cytoplasmic foci which do not appear to overlap with early or late endosomes, the endoplasmic reticulum, the Golgi complex, multivesicular bodies (MVBs), lysosomes, or mitochondria. Often found in close association with microtubules.
Target information above from: UniProt accession Q9UJC3 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 51361 Human
- Omim: 607820 Human
- SwissProt: Q9UJC3 Human
- Unigene: 378836 Human
別名
- A930033L17Rik antibody
- Abnormal spermatozoon head shape antibody
- azh antibody
- h-hook1 antibody
- hHK1 antibody
- HK1 antibody
- HOOK 1 antibody
- Hook homolog 1 (Drosophila) antibody
- Hook1 antibody
- HOOK1_HUMAN antibody
- MGC10642 antibody
- OTTHUMP00000010548 antibody
- OTTMUSP00000008480 antibody
- Protein Hook homolog 1 antibody
- RP23-80B16.4 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)

Immunohistochemical analysis of paraffin embedded Human testis tissue labeling HOOK1 with ab151756 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-HOOK1 antibody [EPR10102] (ab151756)

All lanes : Anti-HOOK1 antibody [EPR10102] (ab151756) at 1/1000 dilution

Lane 1 : 293T cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : Caco-2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 85 kDa
Immunocytochemistry/ Immunofluorescence - Anti-HOOK1 antibody [EPR10102] (ab151756)

Immunofluorescence analysis of Caco-2 cells labeling HOOK1 with ab151756 at a 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)

Immunohistochemical analysis of paraffin embedded Human normal brain tissue using ab151756 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)

Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab151756 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)

Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue using ab151756 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)

Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab151756 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)

Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab151756 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-HOOK1 antibody [EPR10102] (ab151756)

Western blot protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab151756 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab151756 は 5 報の論文で使用されています。

Higashi S et al. Regulation of Hook1-mediated endosomal sorting of clathrin-independent cargo by γ-taxilin. J Cell Sci 135:N/A (2022). PubMed: 34897470
Villari G et al. Distinct retrograde microtubule motor sets drive early and late endosome transport. EMBO J 39:e103661 (2020). PubMed: 33215754
Yang H et al. The clinicopathological and prognostic implications of tyrosine phosphatase SHP2 and ankyrin Hook1 gene expression in non- small cell lung cancer patients treated with gemcitabine plus platinum as first-line chemotherapy. Ann Palliat Med 9:2943-2952 (2020). PubMed: 32819122
Dwivedi D et al. The dynein adaptor Hook2 plays essential roles in mitotic progression and cytokinesis. J Cell Biol 218:871-894 (2019). PubMed: 30674580
Cao J et al. Clinicopathological and prognostic significance of SHP2 and Hook1 expression in patients with thyroid carcinoma. Hum Pathol N/A:N/A (2018). PubMed: 29953894

レビューと Q&A

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Application

Western blot

Sample

Human Cell lysate - whole cell (293T cells)

Gel Running Conditions

Reduced Denaturing (4-12% Bis-Tris gel (20 well))

Loading amount

20 µg

Specification

293T cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Aug 14 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Isotype control

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (5)

レビューと Q&A

Western blot abreview for Anti-HOOK1 antibody [EPR10102]