AB316287

Anti-Homer1 抗体 [RM1121]

Anti-Homer1 antibody [RM1121]

BOND RX™ Validated
20ul selling size
Recombinant
RabMAb
詳細を見る

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Multiclonal Homer1 antibody. Suitable for IHC-P, IHC-Fr, IP, WB and reacts with Human, Mouse, Rat samples.

別名を表示する

SYN47, HOMER1, Homer protein homolog 1, Homer-1

16 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [RM1121] (AB316287)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [RM1121] (AB316287)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Homer1 with ab316287 at 1/1000 (0.518 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on the human cerebrum in ab316287 (Image A) and weak stainning on the human cerebrum in ab97593 (Image B).
The image A is applied with Anti-Homer1 antibody (ab316287) at 1/1000 dilution and the image B is applied with Anti-Homer1 antibody (ab97593) at 1/200 dilution.
The section was incubated with ab316287 or ab97593 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

Supplier Data

Immunoprecipitation - Anti-Homer1 antibody [RM1121] (AB316287)

Homer1 was immunoprecipitated from 0.35 mg Human brain tissue lysate with ab316287 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab316287 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Human brain tissue lysate
Lane 2 : ab316287 IP in Human brain tissue lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab316287 in human brain tissue lysate

All lanes:

Immunoprecipitation - Anti-Homer1 antibody [RM1121] (ab316287) at 1/30 dilution

All lanes:

Human brain tissue lysate with 5% NFDM/TBST

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>)

false

Exposure time: 15s

Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [RM1121] (AB316287)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [RM1121] (AB316287)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh frozen) tissue labeling Homer1 with ab316287 at 1/500 (1.036 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

Panel A : merged staining of anti-Homer1 (ab316287, green), anti-NeuN (ab190565, grey) and anti-GFAP (ab201732, red) on mouse cerebrum.
Panel B : anti-Homer1 stained on mouse cerebrum.
Panel C : anti-NeuN stained in neurons of mouse cerebrum.
Panel D : anti-GFAP stained in astrocytes of mouse cerebrum.
The nuclear counterstain was DAPI (Blue). The section was incubated with ab316287 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [RM1121] (AB316287)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh frozen) tissue labeling Homer1 with ab316287 at 1/500 (1.036 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

Panel A : merged staining of anti-Homer1 (ab316287, green), anti-NeuN (ab190565, grey) and anti-GFAP (ab201732, red) on rat cerebrum.
Panel B : anti-Homer1 stained on rat cerebrum.
Panel C : anti-NeuN stained in neurons of rat cerebrum.
Panel D : anti-GFAP stained in astrocytes of rat cerebrum.
The nuclear counterstain was DAPI (Blue). The section was incubated with ab316287 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [RM1121] (AB316287)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Homer1 with ab316287 at 1/1000 (0.518 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on the mouse cerebrum. The section was incubated with ab316287 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [RM1121] (AB316287)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Homer1 with ab316287 at 1/1000 (0.518 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on the rat cerebrum. The section was incubated with ab316287 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

Supplier Data

Immunoprecipitation - Anti-Homer1 antibody [RM1121] (AB316287)

Homer1 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate with ab316287 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab316287 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse brain tissue lysate
Lane 2 : ab316287 IP in Mouse brain tissue lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab316287 in mouse brain tissue lysate

All lanes:

Immunoprecipitation - Anti-Homer1 antibody [RM1121] (ab316287) at 1/30 dilution

All lanes:

Mouse brain tissue lysate with 5% NFDM/TBST

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>)

false

Exposure time: 8s

Supplier Data

Immunoprecipitation - Anti-Homer1 antibody [RM1121] (AB316287)

Homer1 was immunoprecipitated from 0.35 mg Rat brain tissue lysate with ab316287 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab316287 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Rat brain tissue lysate
Lane 2 : ab316287 IP in Rat brain tissue lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab316287 in rat brain tissue lysate

All lanes:

Immunoprecipitation - Anti-Homer1 antibody [RM1121] (ab316287) at 1/30 dilution

All lanes:

Rat brain tissue lysate with 5% NFDM/TBST

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>)

false

Exposure time: 6s

Supplier Data

Western blot - Anti-Homer1 antibody [RM1121] (AB316287)

Low expression : human heart, human liver and human spleen (PMID : 9808458).

Left Panel is applied with Anti-Homer1 antibody [RM1121] (ab316287) at 1/1000 dilution (0.518 ug/ml); Right panel is applied with Anti-Homer1 antibody (ab97593) at 1/2000 dilution (0.5 ug/ml).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Homer1 antibody [RM1121] (ab316287) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg with 5% NFDM/TBST

Lane 2:

Human heart tissue lysate at 20 µg with 5% NFDM/TBST

Lane 3:

Human liver tissue lysate at 20 µg with 5% NFDM/TBST

Lane 4:

Human spleen tissue lysate at 20 µg with 5% NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 42 kDa,36 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-Homer1 antibody [RM1121] (AB316287)

Low expression : rat heart, rat kidney and rat spleen.

Left Panel is applied with Anti-Homer1 antibody [RM1121] (ab316287) at 1/1000 dilution (0.518 ug/ml); Right panel is applied with Anti-Homer1 antibody (ab97593) at 1/2000 dilution (0.5 ug/ml).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Homer1 antibody [RM1121] (ab316287) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 20 µg with 5% NFDM/TBST

Lane 2:

Rat heart tissue lysate at 20 µg with 5% NFDM/TBST

Lane 3:

Rat kidney tissue lysate at 20 µg with 5% NFDM/TBST

Lane 4:

Rat spleen tissue lysate at 20 µg with 5% NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 41 kDa,36 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-Homer1 antibody [RM1121] (AB316287)

Low expression : mouse heart, mouse kidney and mouse spleen.

Left Panel is applied with Anti-Homer1 antibody [RM1121] (ab316287) at 1/1000 dilution (0.518 ug/ml); Right panel is applied with Anti-Homer1 antibody (ab97593) at 1/2000 dilution (0.5 ug/ml).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Homer1 antibody [RM1121] (ab316287) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg with 5% NFDM/TBST

Lane 2:

Mouse heart tissue lysate at 20 µg with 5% NFDM/TBST

Lane 3:

Mouse kidney tissue lysate at 20 µg with 5% NFDM/TBST

Lane 4:

Mouse spleen tissue lysate at 20 µg with 5% NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 41 kDa,36 kDa

false

Exposure time: 180s

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [RM1121] (AB316287)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh frozen) tissue labeling Homer1 with ab316287 at 1/500 (1.036 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

Negative control : confocal image showing no staining on rat liver. The nuclear counterstain was DAPI (Blue). The section was incubated with ab316287 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [RM1121] (AB316287)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Homer1 with ab316287 at 1/1000 (0.518 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on the mouse liver (PMID : 10651798). The section was incubated with ab316287 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [RM1121] (AB316287)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Homer1 with ab316287 at 1/1000 (0.518 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on the human liver. The section was incubated with ab316287 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [RM1121] (AB316287)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Homer1 with ab316287 at 1/1000 (0.518 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on the rat liver. The section was incubated with ab316287 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [RM1121] (AB316287)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh frozen) tissue labeling Homer1 with ab316287 at 1/500 (1.036 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

Negative control : confocal image showing no staining on mouse liver (PMID : 10651798). The nuclear counterstain was DAPI (Blue). The section was incubated with ab316287 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.

Key facts

宿主種

Rabbit

クローン性

Multiclonal

クローン番号

RM1121

アイソタイプ

IgG

キャリアフリー

交差種

Human, Mouse, Rat

アプリケーション

IHC-Fr, IP, WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

style

left-column

style

left-column

製品の詳細

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Homer1 also known as HOMER is a scaffolding protein with a mass of approximately 45 kDa. It functions in organizing receptor signaling complexes notably interacting with metabotropic glutamate receptors (mGluRs) and certain transient receptor potential (TRP) channels. Homer1 exists mainly in the postsynaptic density of excitatory synapses in the brain indicating its strong expression in neuronal tissues. It has multiple isoforms with the isoform 1a often being highlighted in various research contexts.

Biological function summary

Homer1 plays an important role in synaptic plasticity and intracellular calcium signaling. As part of a complex Homer1 aids in linking mGluRs to other proteins stabilizing receptor signaling and contributing to the regulation of calcium homeostasis within the cell. This scaffolding action has implications for the modulation of signal transduction pathways that maintain neuronal communication and synaptic efficacy.

Pathways

Homer1 interacts significantly with the mGluR signaling pathway and the calcium signaling pathway. These interactions place Homer1 at a nexus of neuronal signal propagation. Related proteins in this context include the mGluR1 and mGluR5 which in sync with Homer1 form a network ensuring proper synaptic response to neurotransmitters. TRPC channels are also closely related as Homer1 helps modulate their activity through dynamic assembly with mGluR complexes.

Homer1 shows involvement in various neurological conditions such as schizophrenia and autism spectrum disorders. Abnormalities in Homer1 expression or function are linked to disrupted synaptic signaling which contributes to the pathophysiology of these disorders. Within these disease frameworks proteins like mGluR5 collaborate with Homer1 and serve as focal points in understanding the disrupted signaling mechanisms underlying these conditions.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development. Forms a high-order complex with SHANK1, which in turn is necessary for the structural and functional integrity of dendritic spines (By similarity). Negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway. Acts by competing with calcineurin/PPP3CA for NFAT protein binding, hence preventing NFAT activation by PPP3CA (PubMed : 18218901).

See full target information HOMER1

style

left-column

style

left-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

style

left-column

style

right-column

Anti-Homer1 抗体 [RM1121]

Anti-Homer1 antibody [RM1121]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [RM1121] (AB316287)

Immunoprecipitation - Anti-Homer1 antibody [RM1121] (AB316287)

Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [RM1121] (AB316287)

Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [RM1121] (AB316287)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [RM1121] (AB316287)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [RM1121] (AB316287)

Immunoprecipitation - Anti-Homer1 antibody [RM1121] (AB316287)

Immunoprecipitation - Anti-Homer1 antibody [RM1121] (AB316287)

Western blot - Anti-Homer1 antibody [RM1121] (AB316287)

Western blot - Anti-Homer1 antibody [RM1121] (AB316287)

Western blot - Anti-Homer1 antibody [RM1121] (AB316287)

Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [RM1121] (AB316287)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [RM1121] (AB316287)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [RM1121] (AB316287)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [RM1121] (AB316287)

Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [RM1121] (AB316287)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

Abcam product promise

関連製品

Alternative Version

Primary Antibodies

Anti-Homer1 antibody [RM1121] - BSA and Azide free