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リコンビナントRabMAb

Anti-Homer1 抗体 [EPR15309] (ab184955)

Reviews (2) Submit a question References (17)
Western blot - Anti-Homer1 antibody [EPR15309] (ab184955)
  • Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (ab184955)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [EPR15309] (ab184955)
  • Immunoprecipitation - Anti-Homer1 antibody [EPR15309] (ab184955)
  • Western blot - Anti-Homer1 antibody [EPR15309] (ab184955)
  • Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (ab184955)
  • Anti-Homer1 antibody [EPR15309] (ab184955)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15309] to Homer1
  • Suitable for: IP, WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Homer1 antibody [EPR15309]
    Homer1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR15309] to Homer1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IP, WB, IHC-P, IHC-Frmore details
    適用なし: ICC

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HepG2 and human fetal brain lysates. IP: Human fetal brain lysate. IHC-P: Human gliocytoma tissue. IHC-Fr: Rat and mouse cerebrum tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184955の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
1/60 - 1/80.
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr (1)
1/250.
特記事項
IP
1/60 - 1/80.
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/250.
追加情報
Is unsuitable for ICC.

ターゲット情報

  • 機能

    Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development.

  • 配列類似性

    Belongs to the Homer family.
    Contains 1 WH1 domain.

  • ドメイン

    The WH1 domain interacts with the PPXXF motif in GRM1, GRM5, RYR1, RYR2, ITPR1, SHANK 1 and SHANK3.

  • 細胞内局在

    Cytoplasm. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Isoform 1 inhibits surface expression of GRM5 causing it to be retained in the endoplasmic reticulum.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • HOME1_HUMAN antibody
    • Homer antibody
    • HOMER 1A antibody
    • HOMER 1B antibody
    • HOMER 1C antibody
    • Homer homolog 1 (Drosophila) antibody
    • homer homolog 1 antibody
    • Homer protein homolog 1 antibody
    • homer scaffolding protein 1 antibody
    • Homer, Drosophila, homolog of, 1 antibody
    • Homer, neuronal immediate early gene, 1 antibody
    • Homer-1 antibody
    • Homer1 antibody
    • HOMER1A antibody
    • HOMER1B antibody
    • HOMER1C antibody
    • SYN47 antibody
    • Ves 1 antibody
    see all

画像

  • Western blot - Anti-Homer1 antibody [EPR15309] (ab184955)
    Western blot - Anti-Homer1 antibody [EPR15309] (ab184955)
    Anti-Homer1 antibody [EPR15309] (ab184955) at 1/5000 dilution + HepG2 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 40 kDa
    Additional bands at: 46 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (ab184955)
    Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (ab184955)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum tissue labeling Homer1 with ab184955 at 1/250 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on rat cerebrum. is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 (2 ug/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [EPR15309] (ab184955)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [EPR15309] (ab184955)

    Immunohistochemical analysis of paraffin-embedded human gliocytoma labeling Homer with ab184955 at 1/500 dilution and HRP polymer for Rabbit IgG.  Counterstained with hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Homer1 antibody [EPR15309] (ab184955)
    Immunoprecipitation - Anti-Homer1 antibody [EPR15309] (ab184955)

    Immunoprecipitation analysis of human fetal brain labeling Homer with ab184955 at 1/50 dilution and Anti-rabbit IgG (HRP) specific to the non reduced form of IgG at 1/1500 dilution (+) or PBS (-)

  • Western blot - Anti-Homer1 antibody [EPR15309] (ab184955)
    Western blot - Anti-Homer1 antibody [EPR15309] (ab184955)
    Anti-Homer1 antibody [EPR15309] (ab184955) at 1/20000 dilution + Human fetal brain lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 40 kDa
    Additional bands at: 46 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (ab184955)
    Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (ab184955)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling Homer1 with ab184955 at 1/250 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse cerebrum. is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 (2 ug/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Anti-Homer1 antibody [EPR15309] (ab184955)
    Anti-Homer1 antibody [EPR15309] (ab184955)

参考文献 (17)

ab184955 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab184955 は 17 報の論文で使用されています。

  • Yokokawa T  et al. Altered expression of synaptic proteins and adhesion molecules in the hippocampus and cortex following the onset of diabetes in nonobese diabetic mice. Physiol Rep 11:e15673 (2023). PubMed: 37078449
  • Ge J  et al. MiR-431 attenuates synaptic plasticity and memory deficits in APPswe/PS1dE9 mice. JCI Insight 8:N/A (2023). PubMed: 37192007
  • Damstra HGJ  et al. Visualizing cellular and tissue ultrastructure using Ten-fold Robust Expansion Microscopy (TREx). Elife 11:N/A (2022). PubMed: 35179128
  • Franco-García A  et al. Molecular Mechanisms Underlying the Retrieval and Extinction of Morphine Withdrawal-Associated Memories in the Basolateral Amygdala and Dentate Gyrus. Biomedicines 10:N/A (2022). PubMed: 35327388
  • Sommer D  et al. Aging-Dependent Altered Transcriptional Programs Underlie Activity Impairments in Human C9orf72-Mutant Motor Neurons. Front Mol Neurosci 15:894230 (2022). PubMed: 35774867
View all Publications for this product

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-Homer1 antibody [EPR15309]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Hippocampus)
Gel Running Conditions
Reduced Denaturing (Gel 10%)
Loading amount
1 µg
Specification
Hippocampus
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jun 27 2017

Immunohistochemistry (Frozen sections) abreview for Anti-Homer1 antibody [EPR15309]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Mouse spinal cord)
Permeabilization
Yes - 0.1% PBS Triton X-100
Specification
Mouse spinal cord
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 18 2015

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