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AB315797

Anti-Homeobox protein cut-like 2 抗体 [EPR26509-79] - BSA and Azide free

Anti-Homeobox protein cut-like 2 antibody [EPR26509-79] - BSA and Azide free

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Rabbit Recombinant Monoclonal CUX2 antibody. Carrier free. Suitable for IHC-P and reacts with Mouse, Rat, Transfected cell line - Mouse samples.

別名を表示する

Cutl2, Cux2, Homeobox protein cut-like 2, Homeobox protein Cux-2

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 2 antibody [EPR26509-79] - BSA and Azide free (AB315797)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 2 antibody [EPR26509-79] - BSA and Azide free (AB315797)

This data was developed using ab315796, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a mouse CUX2 expression vector containing a His tag. (B) HEK-293T (human embryonic kidney epithelial cell) transfected with a mouse CUX1 expression vector containing a His tag. (C) HEK-293T transfected with empty vector containing a His tag. tissue labeling Homeobox protein cut-like 2 with ab315796 at 1/100 (5.16 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on HEK-293T transfected with a His-tagged mouse CUX2 construct (image A). No staining on HEK-293T transfected with a His-tagged mouse CUX1 construct (image B). No staining on HEK-293T transfected with empty plasmid (image C). The section was incubated with ab315796 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 2 antibody [EPR26509-79] - BSA and Azide free (AB315797)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 2 antibody [EPR26509-79] - BSA and Azide free (AB315797)

This data was developed using ab315796, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling Homeobox protein cut-like 2 with ab315796 at 1/100 (5.16 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on rat lung. The section was incubated with ab315796 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 2 antibody [EPR26509-79] - BSA and Azide free (AB315797)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 2 antibody [EPR26509-79] - BSA and Azide free (AB315797)

This data was developed using ab315796, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Homeobox protein cut-like 2 with ab315796 at 1/100 (5.16 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse lung (PMID : 8798433). The section was incubated with ab315796 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 2 antibody [EPR26509-79] - BSA and Azide free (AB315797)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 2 antibody [EPR26509-79] - BSA and Azide free (AB315797)

This data was developed using ab315796, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Homeobox protein cut-like 2 with ab315796 at 1/100 (5.16 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on rat liver. The section was incubated with ab315796 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 2 antibody [EPR26509-79] - BSA and Azide free (AB315797)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 2 antibody [EPR26509-79] - BSA and Azide free (AB315797)

This data was developed using ab315796, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Homeobox protein cut-like 2 with ab315796 at 1/100 (5.16 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse spleen (PMID : 8798433). The section was incubated with ab315796 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 2 antibody [EPR26509-79] - BSA and Azide free (AB315797)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 2 antibody [EPR26509-79] - BSA and Azide free (AB315797)

This data was developed using ab315796, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Homeobox protein cut-like 2 with ab315796 at 1/100 (5.16 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat cerebrum. The section was incubated with ab315796 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 2 antibody [EPR26509-79] - BSA and Azide free (AB315797)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 2 antibody [EPR26509-79] - BSA and Azide free (AB315797)

This data was developed using ab315796, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Homeobox protein cut-like 2 with ab315796 at 1/100 (5.16 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse cerebrum (PMID : 8798433). The section was incubated with ab315796 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

関連する標識済み抗体及び組成の異なる製品 (1)

  • Unconjugated

    Anti-Homeobox protein cut-like 2 antibody [EPR26509-79]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR26509-79

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Mouse, Rat

アプリケーション

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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製品の詳細

ab315797 is the carrier-free version of ab315796.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The Homeobox protein cut-like 2 also known as CDP CUX2 or Clox functions as a transcription factor and plays a critical role in regulating gene expression. Mechanically it binds to specific DNA sequences to influence the transcription of target genes. CUX2 has a molecular mass of approximately 150 kDa. Researchers observe its expression in the brain particularly in developing neocortex as well as the adult testis and lymph nodes.
Biological function summary

Homeobox protein cut-like 2 is involved in the development of the nervous system and the regulation of cell growth and differentiation. It is not part of a larger complex but interacts with other transcription factors to modulate these processes. The protein's involvement is significant in neural development impacting neurogenesis and neural patterning. It coordinates with DNA repair pathways as well.

Pathways

Homeobox protein cut-like 2 integrates into neural developmental pathways and cell cycle regulation. In neural development it interacts with other factors like NEUROD1 to influence neuronal differentiation. In cell cycle control it participates in pathways such as the cell proliferation pathway interacting with proteins like p27Kip1 to regulate cell cycle progression and ensure proper cell growth.

Homeobox protein cut-like 2 has an association with neurodevelopmental disorders and cancer. Altered expression or mutations in the protein can lead to developmental abnormalities including neural developmental issues. In cancer abnormal regulation can contribute to tumorigenesis especially in brain-related cancers. It might interact with proteins associated with these conditions such as the tumor suppressor protein p53 indicating its significance in disease mechanisms.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Transcription factor involved in the control of neuronal proliferation and differentiation in the brain (PubMed : 18033766, PubMed : 20510857). Regulates dendrite development and branching, dendritic spine formation, and synaptogenesis in cortical layers II-III (PubMed : 20510857). Binds to DNA in a sequence-specific manner.
See full target information Cux2
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Abcam product promise

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