Anti-hnRNP U/p120 抗体 [EPR12279] (ab172608)
![Western blot - Anti-hnRNP U/p120 antibody [EPR12279] (ab172608)](https://www.abcam.co.jp/ps/products/172/ab172608/Images/ab172608-195590-anti-hnrnp-u-p120-antibody-epr12279-western-blot.jpg "Western blot - Anti-hnRNP U/p120 antibody [EPR12279] (ab172608)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12279] to hnRNP U/p120
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-hnRNP U/p120 antibody [EPR12279]
hnRNP U/p120 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR12279] to hnRNP U/p120 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- K562, Jurkat and 293T cell lysates; Human breast carcinoma and thyroid gland carcinoma tissues; HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR12279 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab172608の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 91 kDa.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/250 - 1/500.
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| 特記事項 |
|---|
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WB
1/1000 - 1/5000. Predicted molecular weight: 91 kDa. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/250 - 1/500. |
ターゲット情報
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機能
Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Binds to pre-mRNA. Has high affinity for scaffold-attached region (SAR) DNA. Binds to double- and single-stranded DNA and RNA. -
配列類似性
Contains 1 B30.2/SPRY domain.
Contains 1 SAP domain. -
翻訳後修飾
Extensively phosphorylated.
Arg-733 and Arg-739 are dimethylated, probably to asymmetric dimethylarginine. -
細胞内局在
Nucleus. Cytoplasm. Cell surface. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Also found associated with the cell surface. - Information by UniProt
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参照データベース
- Entrez Gene: 3192 Human
- Omim: 602869 Human
- SwissProt: Q00839 Human
- Unigene: 106212 Human
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別名
- Heterogeneous nuclear ribonucleoprotein U antibody
- hnRNP U antibody
- hnRNP U protein antibody
see all
画像
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Western blot - Anti-hnRNP U/p120 antibody [EPR12279] (ab172608)All lanes : Anti-hnRNP U/p120 antibody [EPR12279] (ab172608) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 91 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP U/p120 antibody [EPR12279] (ab172608)](https://www.abcam.co.jp/ps/products/172/ab172608/Images/ab172608-195591-anti-hnrnp-u-p120-antibody-epr12279-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP U/p120 antibody [EPR12279] (ab172608)Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labeling hnRNP U/p120 with ab172608 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP U/p120 antibody [EPR12279] (ab172608)](https://www.abcam.co.jp/ps/products/172/ab172608/Images/ab172608-195592-anti-hnrnp-u-p120-antibody-epr12279-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP U/p120 antibody [EPR12279] (ab172608)Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue labeling hnRNP U/p120 with ab172608 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-hnRNP U/p120 antibody [EPR12279] (ab172608)](https://www.abcam.co.jp/ps/products/172/ab172608/Images/ab172608-195593-anti-hnrnp-u-p120-antibody-epr12279-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP U/p120 antibody [EPR12279] (ab172608)Immunofluorescent staining of HeLa cells labeling hnRNP U/p120 with ab172608 at 1/250 dilution.
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![Anti-hnRNP U/p120 antibody [EPR12279] (ab172608)](https://www.abcam.co.jp/ps/products/172/ab172608/Images/ab172608-5-benefits-of-recombinant-antibodies.png)
Anti-hnRNP U/p120 antibody [EPR12279] (ab172608)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab172608 は論文での使用が確認できていません。