Anti-hnRNP K 抗体 (ab70492)
Key features and details
- Rabbit polyclonal to hnRNP K
- Suitable for: WB, IP, ICC/IF, IHC-P, ChIP/Chip
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-hnRNP K antibody
hnRNP K 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to hnRNP K -
由来種
Rabbit -
特異性
This antibody does not appear to react with isoform b of human hnRNPK (NP_112552.1). -
アプリケーション
適用あり: WB, IP, ICC/IF, IHC-P, ChIP/Chipmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Guinea pig, Cow, Rhesus monkey, Gorilla, Orangutan, Platypus, Elephant
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免疫原
Synthetic peptide corresponding to a sequence from the C-terminus of isoform a of human hnRNP K. (NP_002131.2)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70492の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).
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| IP |
Use at 1-4 µg/mg of lysate.
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| ICC/IF |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration.
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| ChIP/Chip |
Use at an assay dependent concentration. PubMed: 20200201
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| 特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa). |
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IP
Use at 1-4 µg/mg of lysate. |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
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ChIP/Chip
Use at an assay dependent concentration. PubMed: 20200201 |
ターゲット情報
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機能
One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. -
配列類似性
Contains 3 KH domains. -
翻訳後修飾
Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine. -
細胞内局在
Cytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear. - Information by UniProt
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参照データベース
- Entrez Gene: 528135 Cow
- Entrez Gene: 3190 Human
- Entrez Gene: 15387 Mouse
- Entrez Gene: 117282 Rat
- Entrez Gene: 709112 Rhesus monkey
- Omim: 600712 Human
- SwissProt: Q3T0D0 Cow
- SwissProt: P61978 Human
see all -
別名
- CSBP antibody
- dC stretch binding protein antibody
- FLJ41122 antibody
see all
画像
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Western blot - Anti-hnRNP K antibody (ab70492)All lanes : Anti-hnRNP K antibody (ab70492) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 51 kDa
Observed band size: 51,55 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody (ab70492)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling hnRNP K with ab70492 at 1/1000 (0.2µg/ml). Detection: DAB. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody (ab70492)IHC image of hnRNP K staining in Human Colon Adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70492, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunoprecipitation - Anti-hnRNP K antibody (ab70492)Detection of Human hnRNP K by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg/IP, 20% of IP loaded) using ab70492 at 3 µg/mg for IP and at 1.0 µg/ml for subsequent WB detection. Lane 2 represents IP IgG control.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab70492 は 13 報の論文で使用されています。
- Zhang T et al. LncRNA GAS5-hnRNPK axis inhibited ovarian cancer progression via inhibition of AKT signaling in ovarian cancer cells. Discov Oncol 14:157 (2023). PubMed: 37639158
- Avar M et al. An arrayed genome-wide perturbation screen identifies the ribonucleoprotein Hnrnpk as rate-limiting for prion propagation. EMBO J 41:e112338 (2022). PubMed: 36254605
- Xu W et al. SnoRD126 promotes the proliferation of hepatocellular carcinoma cells through transcriptional regulation of FGFR2 activation in combination with hnRNPK. Aging (Albany NY) 13:13300-13317 (2021). PubMed: 33891563
- Richter WF et al. Non-canonical H3K79me2-dependent pathways promote the survival of MLL-rearranged leukemia. Elife 10:N/A (2021). PubMed: 34263728
- Kwon PK et al. hnRNP K supports the maintenance of RORγ circadian rhythm through ERK signaling. FASEB J 35:e21507 (2021). PubMed: 33724572