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Anti-hnRNP K 抗体 [3C2] (ab39975)

Reviews (5) Submit a question References (37)
Western blot - Anti-hnRNP K antibody [3C2] (ab39975)
  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [3C2] (ab39975)
  • Flow Cytometry - Anti-hnRNP K antibody [3C2] (ab39975)

Key features and details

  • Mouse monoclonal [3C2] to hnRNP K
  • Suitable for: IP, ELISA, ChIP/Chip, WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Hamster, Cow, Human, Xenopus laevis
  • Isotype: IgG2b

製品の概要

  • 製品名

    Anti-hnRNP K antibody [3C2]
    hnRNP K 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [3C2] to hnRNP K

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IP, ELISA, ChIP/Chip, WB, ICC/IF, Flow Cytmore details

  • 種交差性

    交差種: Mouse, Rat, Hamster, Cow, Human, Xenopus laevis

  • 免疫原

    Full length native protein (purified) (Human)

  • ポジティブ･コントロール

    • HeLa extracts. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab39975の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP (1)
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
ChIP/Chip
Use at an assay dependent concentration. PubMed: 20673990
WB (1)
1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 51 kDa).
ICC/IF (1)
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. 
特記事項
IP
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
ChIP/Chip
Use at an assay dependent concentration. PubMed: 20673990
WB
1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 51 kDa).
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. 

ターゲット情報

  • 機能

    One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA.

  • 配列類似性

    Contains 3 KH domains.

  • 翻訳後修飾

    Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.

  • 細胞内局在

    Cytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • CSBP antibody
    • dC stretch binding protein antibody
    • FLJ41122 antibody
    • Heterogeneous nuclear ribonucleoprotein K antibody
    • hnRNP K antibody
    • HNRNPK antibody
    • HNRPK antibody
    • HNRPK_HUMAN antibody
    • Transformation up regulated nuclear protein antibody
    • Transformation up-regulated nuclear protein antibody
    • Transformation upregulated nuclear protein antibody
    • TUNP antibody
    see all

画像

  • Western blot - Anti-hnRNP K antibody [3C2] (ab39975)
    Western blot - Anti-hnRNP K antibody [3C2] (ab39975)
    All lanes : Anti-hnRNP K antibody [3C2] (ab39975) at 1/1000 dilution

    Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
    Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 51 kDa
    Observed band size: 55 kDa why is the actual band size different from the predicted?
    Additional bands at: 65 kDa (possible post-translational modification)

  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [3C2] (ab39975)
    Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [3C2] (ab39975)

    ICC/IF image of ab39975 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab39975 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Flow Cytometry - Anti-hnRNP K antibody [3C2] (ab39975)
    Flow Cytometry - Anti-hnRNP K antibody [3C2] (ab39975)
    Overlay histogram showing HeLa cells stained with ab39975 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab39975, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

参考文献 (37)

ab39975 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab39975 は 37 報の論文で使用されています。

  • Cao C  et al. Enhancement of protein translation by CRISPR/dCasRx coupled with SINEB2 repeat of noncoding RNAs. Nucleic Acids Res 51:e33 (2023). PubMed: 36715335
  • Li KQ  et al. EPAS1 prevents telomeric damage-induced senescence by enhancing transcription of TRF1, TRF2, and RAD50. Zool Res 44:636-649 (2023). PubMed: 37070589
  • Tan Y  et al. A novel oncogenic seRNA promotes nasopharyngeal carcinoma metastasis. Cell Death Dis 13:401 (2022). PubMed: 35461306
  • Ruff SE  et al. PIM1 phosphorylation of the androgen receptor and 14-3-3 ? regulates gene transcription in prostate cancer. Commun Biol 4:1221 (2021). PubMed: 34697370
  • Sengupta S  et al. PRMT5 Promotes Symmetric Dimethylation of RNA Processing Proteins and Modulates Activated T Cell Alternative Splicing and Ca2+/NFAT Signaling. Immunohorizons 5:884-897 (2021). PubMed: 34716181
View all Publications for this product

レビューと Q&A

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1-5 of 5 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-hnRNP K antibody [3C2]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Melanoma cells)
Permeabilization
Yes - Triton X 100
Specification
Melanoma cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 08 2021

Immunohistochemistry (Frozen sections) abreview for Anti-hnRNP K antibody [3C2]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Skin)
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 21°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 30 2021

Immunocytochemistry abreview for Anti-hnRNP K antibody [3C2]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (Melanoma cells)
Permeabilization
No
Specification
Melanoma cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 21°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 29 2021

Immunoprecipitation abreview for Anti-hnRNP K antibody [3C2] - ChIP Grade

 Excellent
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Mouse Cell lysate - nuclear (Embryonic stem cells)
Total protein in input
300 µg
Specification
Embryonic stem cells
Immuno-precipitation step
Protein G
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Peter Thompson

Verified customer

投稿 May 25 2012

Western blot abreview for Anti-hnRNP K antibody [3C2] - ChIP Grade

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - nuclear (NSC34)
Loading amount
10 µg
Specification
NSC34
Gel Running Conditions
Reduced Denaturing (4-12% nupage gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 02 2010

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