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リコンビナントRabMAb

Anti-hnRNP H 抗体 [EPR25302-12] (ab289974)

Submit a review Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
  • Flow Cytometry (Intracellular) - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
  • Immunoprecipitation - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
  • Western blot - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
  • Flow Cytometry (Intracellular) - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
  • Immunoprecipitation - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
  • Western blot - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
  • Western blot - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
  • Western blot - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
  • Anti-hnRNP H antibody [EPR25302-12] (ab289974)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25302-12] to hnRNP H
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra), IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-hnRNP H antibody [EPR25302-12]
    hnRNP H 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25302-12] to hnRNP H

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, WB, IHC-P, Flow Cyt (Intra), IPmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa, HEK-293T, U-2OS, MOLT-4, C2C12, NIH/3T3 and C6 whole cell lysates; Mouse testis, colon and liver tissue lysates; Rat liver and testis tissue lysates. IHC-P: Human testis and prostatic hyperplasia tissue; Mouse testis tissue; Rat testis tissue. ICC/IF: HeLa and C2C12 cells Flow Cyt (intra): HeLa and C2C12 cells. IP: HeLa and C2C12 whole cell lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR25302-12

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab289974の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/100.
WB
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt (Intra)
1/500.
IP
1/30.
特記事項
ICC/IF
1/100.
WB
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt (Intra)
1/500.
IP
1/30.

ターゲット情報

  • 機能

    This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Mediates pre-mRNA alternative splicing regulation. Inhibits, together with CUGBP1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Binds to the IR RNA. Binds poly(RG).

  • 組織特異性

    Expressed ubiquitously.

  • 配列類似性

    Contains 3 RRM (RNA recognition motif) domains.

  • ドメイン

    Each quasi-RRM repeat bound poly(RG), while only the N-terminal QRRM bound poly(RC) and poly(RU). None of the repeats bound detectable amounts of poly(RA).

  • 細胞内局在

    Nucleus > nucleoplasm.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • DKFZp686A15170 antibody
    • Heterogeneous nuclear ribonucleoprotein H antibody
    • Heterogeneous nuclear ribonucleoprotein H1 (H) antibody
    • Heterogeneous nuclear ribonucleoprotein H1 antibody
    • HNRH1_HUMAN antibody
    • hnRNP H antibody
    • hnRNPH antibody
    • Hnrnph1 antibody
    • HNRPH 1 antibody
    • HNRPH antibody
    • HNRPH1 protein antibody
    • N-terminally processed antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP H antibody [EPR25302-12] (ab289974)

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labelling hnRNP H with ab289974 at 1/500 dilution followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Positive staining on rat testis. The section was incubated with ab289974 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™, Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Flow Cytometry (Intracellular) - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
    Flow Cytometry (Intracellular) - Anti-hnRNP H antibody [EPR25302-12] (ab289974)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized C2C12 (Mouse myoblasts myoblast) cells labelling hnRNP H with ab289974 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
    Immunocytochemistry/ Immunofluorescence - Anti-hnRNP H antibody [EPR25302-12] (ab289974)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 cells llabeling hnRNP H with ab289974 at 1/100 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in C2C12 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

  • Immunoprecipitation - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
    Immunoprecipitation - Anti-hnRNP H antibody [EPR25302-12] (ab289974)

    hnRNP H was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate with ab289974 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289974 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate 10 µg

    Lane 2: ab289974 IP in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab289974 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds

     

  • Western blot - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
    Western blot - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
    All lanes : Anti-hnRNP H antibody [EPR25302-12] (ab289974) at 1/1000 dilution

    Lane 1 : Human testis tissue lysate
    Lane 2 : Mouse colon tissue lysate
    Lane 3 : Mouse liver tissue lysate
    Lane 4 : Mouse testis tissue lysate
    Lane 5 : Rat liver tissue lysate
    Lane 6 : Rat testis tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 49 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 23667175, PMID: 21915099)

     Exposure times: Lane 1: 3 minutes Lane 2-6: 15 seconds

     

  • Flow Cytometry (Intracellular) - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
    Flow Cytometry (Intracellular) - Anti-hnRNP H antibody [EPR25302-12] (ab289974)

    Intracellular flow analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling hnRNP H with ab289974 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
    Immunocytochemistry/ Immunofluorescence - Anti-hnRNP H antibody [EPR25302-12] (ab289974)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling hnRNP H with ab289974 at 1/100 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000  dilution (Green). Confocal image showing nuclear staining in HeLa cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP H antibody [EPR25302-12] (ab289974)

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labelling hnRNP H with ab289974 at 1/500 dilution followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Positive staining on mouse testis. The section was incubated with ab289974 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™, Polymer Refine Detection) .

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP H antibody [EPR25302-12] (ab289974)

    Immunohistochemical analysis of paraffin-embedded Human prostatic hype tissue labelling hnRNP H with ab289974 at 1/500 dilution followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Positive staining on human prostatic hyperplasia. The section was incubated with ab289974 for 30 mins at room temperatureThe immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™, Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP H antibody [EPR25302-12] (ab289974)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling hnRNP H with ab289974 at 1/500 dilution followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Positive staining on human testis. The section was incubated with ab289974 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™, Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunoprecipitation - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
    Immunoprecipitation - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
    hnRNP H was immunoprecipitated from 0.35 mg C2C12 (Mouse myoblasts myoblast), whole cell lysate 10 µg with ab289974 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289974 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: C2C12 (Mouse myoblasts myoblast), whole cell lysate 10 µg

    Lane 2: ab289974 IP in C2C12 whole cell lysate

    Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab289974 in C2C12 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5.5 seconds

  • Western blot - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
    Western blot - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
    All lanes : Anti-hnRNP H antibody [EPR25302-12] (ab289974) at 1/1000 dilution

    Lane 1 : Myc-tagged human HNRPH1 (HNRNPH1) (NM_005520) recombinant protein (20 ng)
    Lane 2 : Myc-tagged human HNRPH2 (HNRNPH2) (NM_019597) recombinant protein (20 ng)
    Lane 3 : Myc-tagged human hnRNP F (HNRNPF) (NM_001098207) recombinant protein(20 ng)
    Lane 4 : Myc-tagged human HNRPH3 (HNRNPH3) (NM_021644) recombinant protein (20 ng)

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 49 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    This antibody reacts with HNRPH1 and HNRPH2, but does not react with HNRNPF and HNRPH3.

    Exposure time: 114 seconds

     

  • Western blot - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
    Western blot - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
    All lanes : Anti-hnRNP H antibody [EPR25302-12] (ab289974) at 1/1000 dilution

    Lane 1 : HEK-293T (human embryonic kidney epithelial cell) whole cell lysate
    Lane 2 : U-2OS(human bone osteosarcoma epithelial cell), whole cell lysate
    Lane 3 : MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate
    Lane 4 : C2C12 (Mouse myoblasts myoblast), whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 6 : C6 (rat glial tumor glial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 49 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 23667175, PMID: 21915099)

    Exposure times: Lane 1-3: 3 seconds Lane 4-6: 5 seconds

     

  • Western blot - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
    Western blot - Anti-hnRNP H antibody [EPR25302-12] (ab289974)
    All lanes : Anti-hnRNP H antibody [EPR25302-12] (ab289974) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate
    Lane 2 : HeLa transfected with siRNA specifically targeti hnRNP H whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 49 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/ molecular weight  observed is consistent with what has been described in the literature (PMID: 23667175, PMID: 21915099)

    Exposure time: 8 seconds

     

  • Anti-hnRNP H antibody [EPR25302-12] (ab289974)
    Anti-hnRNP H antibody [EPR25302-12] (ab289974)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab289974 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab289974 は 1 報の論文で使用されています。

  • Velayutham S  et al. Novel Anti-Melanoma Compounds Are Efficacious in A375 Cell Line Xenograft Melanoma Model in Nude Mice. Biomolecules 13:N/A (2023). PubMed: 37759675

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