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リコンビナントRabMAb

Anti-hnRNP C1/C2 抗体 [EP3034Y] (ab75822)

Submit a review Submit a question References (2)
Western blot - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822)
  • Western blot - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP3034Y] to hnRNP C1/C2
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-hnRNP C1/C2 antibody [EP3034Y]
    hnRNP C1/C2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP3034Y] to hnRNP C1/C2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse and Rat brain lysate, HeLa, and MCF7 lysate. IHC-P: Human breast cancer tissue, Mouse and Rat testis.

  • 特記事項

    This product has switched from a hybridoma to recombinant production method on 23rd February 2024.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab75822の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P
1/800. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
特記事項
WB
1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P
1/800. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
追加情報
Is unsuitable for Flow Cyt or IP.

ターゲット情報

  • 機能

    Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs that affects mRNA splicing, processing and stability. M6A alters the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch' to facilitate binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621).

  • 配列類似性

    Belongs to the RRM HNRPC family. RALY subfamily.
    Contains 1 RRM (RNA recognition motif) domain.

  • 翻訳後修飾

    Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.
    Sumoylated. Sumoylation reduces affinity for mRNA.

  • 細胞内局在

    Nucleus. Component of ribonucleosomes.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • C1 antibody
    • C2 antibody
    • Heterogeneous nuclear ribonucleoprotein C (C1/C2) antibody
    • Heterogeneous nuclear ribonucleoprotein C antibody
    • Heterogeneous nuclear ribonucleoproteins C1/C2 antibody
    • HNRNP antibody
    • hnRNP C1 / hnRNP C2 antibody
    • hnRNP C1/C2 antibody
    • Hnrnpc antibody
    • HNRPC antibody
    • HNRPC_HUMAN antibody
    • MGC104306 antibody
    • MGC105117 antibody
    • MGC117353 antibody
    • MGC131677 antibody
    • Nuclear ribonucleoprotein particle C1 protein antibody
    • Nuclear ribonucleoprotein particle C2 protein antibody
    • SNRPC antibody
    see all

画像

  • Western blot - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822)
    Western blot - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822)
    All lanes : Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822) at 1/10000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: Lane 1, 60 seconds; Lane 2, 5 seconds; Lane 3, 3 seconds

  • Western blot - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822)
    Western blot - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822)
    Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822) at 1/10000 dilution + MCF7(Human breast adenocarcinoma epithelial cell) whole lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa


    Exposure time: 180 seconds


    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822)

    Immunohistochemical analysis of paraffin-embedded Human breast cancer labeling hnRNP C1/C2 with ab75822 at 1/800 dilution (0.589 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab75822 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822)

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling hnRNP C1/C2 with ab75822 at 1/800 dilution (0.589 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab75822 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EP3034Y] (ab75822)

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling hnRNP C1/C2 with ab75822 at 1/800 dilution (0.589 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab75822 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

参考文献 (2)

ab75822 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab75822 は 2 報の論文で使用されています。

  • Rao GK  et al. T cell LFA-1-induced proinflammatory mRNA stabilization is mediated by the p38 pathway kinase MK2 in a process regulated by hnRNPs C, H1 and K. PLoS One 13:e0201103 (2018). PubMed: 30048492
  • Akhrymuk I  et al. Evasion of the innate immune response: the Old World alphavirus nsP2 protein induces rapid degradation of Rpb1, a catalytic subunit of RNA polymerase II. J Virol 86:7180-91 (2012). WB . PubMed: 22514352

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