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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-hnRNP A1 抗体 [EPR12768] (ab177152)

Submit a review Submit a question References (9)
Western blot - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
  • Western blot - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
  • Flow Cytometry (Intracellular) - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
  • Anti-hnRNP A1 antibody [EPR12768] (ab177152)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12768] to hnRNP A1
  • Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free HRP

製品の概要

  • 製品名

    Anti-hnRNP A1 antibody [EPR12768]
    hnRNP A1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR12768] to hnRNP A1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), IHC-P, WB, ICC/IFmore details
    適用なし: IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • MCF-7, HepG2, K562, HEK-293T, HeLa and Jurkat whole cell lysate (ab7899). Human breast carcinoma and clear cell carcinoma tissue. HepG2 cells. Permeabilized Jurkat cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR12768

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab177152の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa).
ICC/IF
1/100 - 1/250.
特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa).
ICC/IF
1/100 - 1/250.
追加情報
Is unsuitable for IP.

ターゲット情報

  • 機能

    Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication.

  • 配列類似性

    Contains 2 RRM (RNA recognition motif) domains.

  • 翻訳後修飾

    Arg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine.
    Sumoylated.

  • 細胞内局在

    Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes. In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • HNRNPA 1 antibody
    • Helix destabilizing protein antibody
    • Helix-destabilizing protein antibody
    • Heterogeneous nuclear ribonucleoprotein A1 antibody
    • Heterogeneous nuclear ribonucleoprotein A1B protein antibody
    • Heterogeneous nuclear ribonucleoprotein B2 protein antibody
    • Heterogeneous nuclear ribonucleoprotein core protein A1 antibody
    • hnRNP A1 antibody
    • hnRNP core protein A1 antibody
    • HNRNPA1 antibody
    • HNRPA1 antibody
    • MGC102835 antibody
    • Nuclear ribonucleoprotein particle A1 protein antibody
    • ROA1_HUMAN antibody
    • Single strand DNA binding protein UP1 antibody
    • Single strand RNA binding protein antibody
    • Single-strand RNA-binding protein antibody
    see all

画像

  • Western blot - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
    Western blot - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
    All lanes : Anti-hnRNP A1 antibody [EPR12768] (ab177152) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : HNRNPA1 knockout HEK293T cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 38 kDa
    Observed band size: 37 kDa why is the actual band size different from the predicted?



    Lanes 1-3: Merged signal (red and green). Green - ab177152 observed at 37 kDa. Red - loading control ab8245 observed at 36 kDa.

    ab177152 Anti-hnRNP A1 antibody [EPR12768] was shown to specifically react with hnRNP A1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266193 (knockout cell lysate ab256942) was used. Wild-type and hnRNP A1 knockout samples were subjected to SDS-PAGE. ab177152 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [EPR12768] (ab177152)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling hnRNP A1 with ab177152 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
    Western blot - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
    All lanes : Anti-hnRNP A1 antibody [EPR12768] (ab177152) at 1/1000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 38 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
    Immunocytochemistry/ Immunofluorescence - Anti-hnRNP A1 antibody [EPR12768] (ab177152)

    Immunofluorescent analysis of HepG2 cells labeling hnRNP A1 with ab177152 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [EPR12768] (ab177152)

    Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma tissue labeling hnRNP A1 with ab177152 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-hnRNP A1 antibody [EPR12768] (ab177152)
    Flow Cytometry (Intracellular) - Anti-hnRNP A1 antibody [EPR12768] (ab177152)

    Intracellular flow cytometric analysis of permeabilized Jurkatcells labeling hnRNP A1with ab177152 at 1/10 dilution (red), or a rabbit IgG (negative) (green). 

     

     

  • Anti-hnRNP A1 antibody [EPR12768] (ab177152)
    Anti-hnRNP A1 antibody [EPR12768] (ab177152)

参考文献 (9)

ab177152 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab177152 は 9 報の論文で使用されています。

  • Liu D  et al. HNRNPA1-mediated exosomal sorting of miR-483-5p out of renal tubular epithelial cells promotes the progression of diabetic nephropathy-induced renal interstitial fibrosis. Cell Death Dis 12:255 (2021). PubMed: 33692334
  • Erdem M  et al. Identification of an mRNA isoform switch for HNRNPA1 in breast cancers. Sci Rep 11:24444 (2021). PubMed: 34961772
  • Noto PB  et al. Identification of hnRNP-A1 as a pharmacodynamic biomarker of type I PRMT inhibition in blood and tumor tissues. Sci Rep 10:22155 (2020). PubMed: 33335114
  • Mori S  et al. Expanded polyglutamine impairs normal nuclear distribution of fused in sarcoma and poly (rC)-binding protein 1 in Huntington's disease. Neuropathology 39:358-367 (2019). PubMed: 31599037
  • Ke RS  et al. Prognostic value and oncogene function of heterogeneous nuclear ribonucleoprotein A1 overexpression in HBV-related hepatocellular carcinoma. Int J Biol Macromol 129:140-151 (2019). PubMed: 30731163
  • Jia Q  et al. HNRNPA1-mediated 3' UTR length changes of HN1 contributes to cancer- and senescence-associated phenotypes. Aging (Albany NY) 11:4407-4437 (2019). PubMed: 31257225
  • Williams AL  et al. HIF1 mediates a switch in pyruvate kinase isoforms after myocardial infarction. Physiol Genomics 50:479-494 (2018). PubMed: 29652636
  • Ke R  et al. Prognostic value of heterogeneous ribonucleoprotein A1 expression and inflammatory indicators for patients with surgically resected hepatocellular carcinoma: Perspectives from a high occurrence area of hepatocellular carcinoma in China. Oncol Lett 16:3746-3756 (2018). PubMed: 30127985
  • Leong SY  et al. The role of Misshapen NCK-related kinase (MINK), a novel Ste20 family kinase, in the IRES-mediated protein translation of human enterovirus 71. PLoS Pathog 11:e1004686 (2015). PubMed: 25747578

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