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リコンビナントRabMAb

Anti-HNF6 抗体 [EPR14976] (ab186743)

Submit a review Submit a question References (1)
Western blot - Anti-HNF6 antibody [EPR14976] (ab186743)
  • Anti-HNF6 antibody [EPR14976] (ab186743)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14976] to HNF6
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-HNF6 antibody [EPR14976]
    HNF6 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14976] to HNF6

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human fetal liver, human and rat liver tissue lysates; HepG2 and Hepa1-6 whole cell lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab186743の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
特記事項
WB
1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

ターゲット情報

画像

  • Western blot - Anti-HNF6 antibody [EPR14976] (ab186743)
    Western blot - Anti-HNF6 antibody [EPR14976] (ab186743)
    All lanes : Anti-HNF6 antibody [EPR14976] (ab186743) at 1/200 dilution

    Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg
    Lane 2 : Hepa1-6 (Mouse hepatoma epithelial cell) whole cell lysates at 15 µg
    Lane 3 : Rat liver tissue lysates at 15 µg
    Lane 4 : Human liver tissue lysates at 15 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa


    Exposure time: 100 seconds


    Blocking/Diluting buffer and concentration 5% NFDM/TBST

    This antibody shows low affinity on liver samples which should be positive according to PMID 27445394.

  • Anti-HNF6 antibody [EPR14976] (ab186743)
    Anti-HNF6 antibody [EPR14976] (ab186743)

参考文献 (1)

ab186743 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab186743 は 1 報の論文で使用されています。

  • Zhong Y  et al. Tumor Supernatant Derived from Hepatocellular Carcinoma Cells Treated with Vincristine Sulfate have Therapeutic Activity. Eur J Pharm Sci N/A:105557 (2020). PubMed: 32946955

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