Anti-HMW Cytokeratin 抗体 [34bE12] (ab776)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMW Cytokeratin antibody [34bE12] (ab776)](https://www.abcam.co.jp/ps/products/0/ab776/Images/ab776-3-anti-hmw-cytokeratin-antibody-immunohistochemistry-prostate-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMW Cytokeratin antibody [34bE12] (ab776)")
Key features and details
- Mouse monoclonal [34bE12] to HMW Cytokeratin
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-HMW Cytokeratin antibody [34bE12] -
製品の詳細
Mouse monoclonal [34bE12] to HMW Cytokeratin -
由来種
Mouse -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human HMW Cytokeratin. Solubilized keratin extract from human stratum corneum.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: Tissue culture supernatant, 1% BSA -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
34bE12 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab776の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/25 - 1/50.
Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Enzymatic antigen retrieval of paraffin embedded tissues is recommended prior to immunostaining. |
| 特記事項 |
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IHC-P
1/25 - 1/50. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Enzymatic antigen retrieval of paraffin embedded tissues is recommended prior to immunostaining. |
ターゲット情報
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関連性
Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue. There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1 (67), CK2 (65.5), CK3 (64), CK4 (59), CK5 (58), CK6 (56), CK7 (54), CK8 (52.5) and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10 (56.5), CK12 (56), CK13 (53), CK14 (50), CK16( 48), CK17 (46), CK18 (45), CK19(48) and CK20(46). -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 3860 Human
- Entrez Gene: 3861 Human
- Entrez Gene: 3866 Human
- Entrez Gene: 3868 Human
- Omim: 148030 Human
- Omim: 148065 Human
- Omim: 148066 Human
- Omim: 148067 Human
see all -
別名
- CK13 antibody
- CK14 antibody
- CK15 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMW Cytokeratin antibody [34bE12] (ab776)Formalin fixed paraffin embedded human prostate tissue, staining HMW Cytokeratin with ab776 in immunohistochemical analysis
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMW Cytokeratin antibody [34bE12] (ab776)](https://www.abcam.co.jp/ps/products/0/ab776/Images/ab776-3705-anti-hmw-cytokeratin-antibody-34be12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMW Cytokeratin antibody [34bE12] (ab776)ab776 staining human skin tissue sections by IHC-P.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab776 は 2 報の論文で使用されています。
- Hermanova I et al. Genetic manipulation of LKB1 elicits lethal metastatic prostate cancer. J Exp Med 217:N/A (2020). PubMed: 32219437
- Hagman Z et al. miR-205 negatively regulates the androgen receptor and is associated with adverse outcome of prostate cancer patients. Br J Cancer 108:1668-76 (2013). IHC ; Human . PubMed: 23571738