Anti-HMGN5 抗体 (ab186001)
Key features and details
- Rabbit polyclonal to HMGN5
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-HMGN5 antibody -
製品の詳細
Rabbit polyclonal to HMGN5 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Gorilla, Orangutan
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免疫原
Recombinant fragment within Human HMGN5 aa 232-282 (C terminal). The exact sequence is proprietary. NP_110390.1
Database link: P82970 -
ポジティブ･コントロール
- HeLa and 293T whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab186001の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 32 kDa.
|
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| IP |
Use at 2-10 µg/mg of lysate.
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| 特記事項 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 32 kDa. |
|
IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Preferentially binds to euchromatin and modulates cellular transcription by counteracting linker histone-mediated chromatin compaction. -
組織特異性
Ubiquitously expressed. -
配列類似性
Belongs to the HMGN family. -
ドメイン
Specifically targeted by its C-terminus to nucleosomes in euchromatin. -
細胞内局在
Nucleus. Associates with nucleosomes in euchromatin and is largely excluded from constitutive heterochromatin. - Information by UniProt
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参照データベース
- Entrez Gene: 79366 Human
- Omim: 300385 Human
- SwissProt: P82970 Human
- Unigene: 282204 Human
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別名
- High mobility group nucleosome binding domain 5 antibody
- High mobility group nucleosome-binding domain-containing protein 5 antibody
- Hmgn5 antibody
see all
画像
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Western blot - Anti-HMGN5 antibody (ab186001)All lanes : Anti-HMGN5 antibody (ab186001) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 32 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-HMGN5 antibody (ab186001)Detection of Human HMGN5 by Western Blot of Immunoprecipitates.
Lane 1: ab186001 used for immunoprecipitation (IP) at 6 μg/mg of lysate from HeLa cells (1 mg for IP; 20% of IP loaded)
Lane 2: Control IgG.
Detection: Chemiluminescence with an exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab186001 は 1 報の論文で使用されています。
- Xu E et al. Hypoxia-Inducible Factor 1A Upregulates HMGN5 by Increasing the Expression of GATA1 and Plays a Role in Osteosarcoma Metastasis. Biomed Res Int 2019:5630124 (2019). PubMed: 31930127