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AB183042

Anti-HLTF 抗体 [EPR14761]

Anti-HLTF antibody [EPR14761]

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(14 Publications)

Knockout Tested Rabbit Recombinant Monoclonal HLTF antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 14 publications.

別名を表示する

HIP116A, RNF80, SMARCA3, SNF2L3, ZBU1, HLTF, DNA-dependent ATPase/E3 ubiquitin-protein ligase HLTF, DNA-binding protein/plasminogen activator inhibitor 1 regulator, HIP116, Helicase-like transcription factor, RING finger protein 80, RING-type E3 ubiquitin transferase HLTF, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3, Sucrose nonfermenting protein 2-like 3

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLTF antibody [EPR14761] (AB183042)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLTF antibody [EPR14761] (AB183042)

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling HLTF with ab183042 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-HLTF antibody [EPR14761] (AB183042)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HLTF antibody [EPR14761] (AB183042)

Immunofluorescent analysis of Jurkat cells (-20°C Acetone-fixed) labeling HLTF with ab183042 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLTF antibody [EPR14761] (AB183042)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLTF antibody [EPR14761] (AB183042)

Immunohistochemical analysis of paraffin-embedded Human infiltrating duct carcinoma of breast tissue labeling HLTF with ab183042 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Western blot - Anti-HLTF antibody [EPR14761] (AB183042)
  • WB

Lab

Western blot - Anti-HLTF antibody [EPR14761] (AB183042)

Lanes 1 - 3 : Merged signal (red and green). Green - ab183042 observed at 113 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab183042 was shown to specifically react with HLTF in wild-type HAP1 cells as signal was lost in HLTF knockout cells. Wild-type and HLTF knockout samples were subjected to SDS-PAGE. ab183042 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-HLTF antibody [EPR14761] (ab183042) at 1 µg

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

HLTF knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HeLa whole cell lysate at 20 µg

Predicted band size: 113 kDa

Observed band size: 113 kDa

false

Western blot - Anti-HLTF antibody [EPR14761] (AB183042)
  • WB

Supplier Data

Western blot - Anti-HLTF antibody [EPR14761] (AB183042)

All lanes:

Western blot - Anti-HLTF antibody [EPR14761] (ab183042) at 1/50000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

K562 lysate at 10 µg

Lane 3:

Jurkat lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 113 kDa,32 kDa

true

関連する標識済み抗体及び組成の異なる製品 (8)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR14761

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, ICC/IF, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Functions as a DNA-dependent ATPase and E3 ubiquitin-protein ligase involved in chromatin regulation and DNA damage tolerance (DDT) (PubMed : 18316726, PubMed : 18719106, PubMed : 26051180, PubMed : 31960921, PubMed : 39142279, PubMed : 40680746). Catalyzes 'Lys-63'-linked polyubiquitination of monoubiquitinated PCNA at 'Lys-164' in response to genotoxic stress, promoting error-free postreplication repair via template switching (PubMed : 18316726, PubMed : 18719106). Acts as an epigenetic regulator by promoting recruitment of DNMT1, thereby ensuring DNA methylation inheritance : specifically binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and mediates histone H3 'Lys-23' polyubiquitination (H3K23ub), a docking site for DNMT1, leading to DNMT1 recruitment and replication-coupled DNA methylation maintenance (PubMed : 40680746). Catalyzes formation of H3K23ub in two steps : first mediates monoubiquitination together with UBE2E1 and UBE2D2, and then extends ubiquitin chains via 'Lys-63'-linked ubiquitination together with UBE2N and UBE2V2 (PubMed : 40680746). Also acts as a chromatin redodeling factor, thereby regulating transcription (PubMed : 10391891, PubMed : 1994885, PubMed : 9126292). Exhibits ATP-dependent double-stranded DNA (dsDNA) translocase activity but lacks classical helicase activity; mediates replication fork reversal by concertedly unwinding and annealing nascent and parental strands, thereby suppressing DNA synthesis and maintaining genomic stability (PubMed : 1994885). Resolves G-quadruplex (G4) DNA structures in cooperation with MSH2, limiting replication stress and G4 accumulation across the cell cycle (PubMed : 39142279). Contributes to nucleotide excision repair by evicting lesion-containing oligonucleotides using its HIRAN and ATPase domains (PubMed : 26051180). Can displace single-stranded DNA from triplex structures through ATP-dependent dsDNA translocation (PubMed : 26051180, PubMed : 31960921). Also has protein clearing activity at the stalled replication fork, facilitating restart of DNA replication (PubMed : 21795603).
See full target information HLTF
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文献 (14)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 52:13003-13018 PubMed39413208

2024

SNF2L suppresses nascent DNA gap formation to promote DNA synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Anthony Nelligan,Huzefa Dungrawala

Gynecologic and obstetric investigation 90:108-119 PubMed39348822

2024

HLTF/SERPINE1 Axis Plays a Crucial Pro-Oncogenic Role in the Progression from Cervical Precancerous Lesions to Cervical Carcinoma in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Wang,Yudi Tan,Shasha Yang,Jinkong Wei,Yuying Wei,Junying Chen

Molecular cell 84:3044-3060.e11 PubMed39142279

2024

HLTF resolves G4s and promotes G4-induced replication fork slowing to maintain genome stability.

Applications

Unspecified application

Species

Unspecified reactive species

Gongshi Bai,Theresa Endres,Ulrike Kühbacher,Valentina Mengoli,Briana H Greer,Emma M Peacock,Matthew D Newton,Tyler Stanage,Maria Rosaria Dello Stritto,Roxana Lungu,Magdalena P Crossley,Ataya Sathirachinda,David Cortez,Simon J Boulton,Petr Cejka,Brandt F Eichman,Karlene A Cimprich

Nucleic acids research 52:3740-3760 PubMed38321962

2024

The levels of p53 govern the hierarchy of DNA damage tolerance pathway usage.

Applications

Unspecified application

Species

Unspecified reactive species

Bryan A Castaño,Sabrina Schorer,Yitian Guo,Nicolás L Calzetta,Vanesa Gottifredi,Lisa Wiesmüller,Stephanie Biber

Molecular cell 83:2357-2366.e8 PubMed37295432

2023

Integrator facilitates RNAPII removal to prevent transcription-replication collisions and genome instability.

Applications

Unspecified application

Species

Unspecified reactive species

Rahul Bhowmick,Kavi P M Mehta,Mads Lerdrup,David Cortez

The Journal of cell biology 222: PubMed37036693

2023

RFWD3 promotes ZRANB3 recruitment to regulate the remodeling of stalled replication forks.

Applications

Unspecified application

Species

Unspecified reactive species

Chandler E Moore,Selin E Yalcindag,Hanna Czeladko,Ramya Ravindranathan,Yodhara Wijesekara Hanthi,Juliana C Levy,Vincenzo Sannino,Detlev Schindler,Alberto Ciccia,Vincenzo Costanzo,Andrew E H Elia

Science advances 8:eabm0314 PubMed35353580

2022

CHK1 phosphorylates PRIMPOL to promote replication stress tolerance.

Applications

Unspecified application

Species

Unspecified reactive species

Kavi P M Mehta,Vaughn Thada,Runxiang Zhao,Archana Krishnamoorthy,Micheal Leser,Kristie Lindsey Rose,David Cortez

Cell death & disease 12:941 PubMed34645785

2021

p53 isoforms differentially impact on the POLι dependent DNA damage tolerance pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yitian Guo,Melanie Rall-Scharpf,Jean-Christophe Bourdon,Lisa Wiesmüller,Stephanie Biber

Molecular cell 81:3007-3017.e5 PubMed34107305

2021

RADX prevents genome instability by confining replication fork reversal to stalled forks.

Applications

Unspecified application

Species

Unspecified reactive species

Archana Krishnamoorthy,Jessica Jackson,Taha Mohamed,Madison Adolph,Alessandro Vindigni,David Cortez

Molecular cell 78:1237-1251.e7 PubMed32442397

2020

HLTF Promotes Fork Reversal, Limiting Replication Stress Resistance and Preventing Multiple Mechanisms of Unrestrained DNA Synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Gongshi Bai,Chames Kermi,Henriette Stoy,Carl J Schiltz,Julien Bacal,Angela M Zaino,M Kyle Hadden,Brandt F Eichman,Massimo Lopes,Karlene A Cimprich
View all publications
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