AB126624

Anti-HLA F 抗体 [EPR6803]

Name: Anti-HLA F 抗体 [EPR6803] (ab126624) | Abcam
Brand: Abcam Limited
SKU: AB126624
Price: 43000 JPY
Availability: InStock
Rating: 4 (1 reviews)

Anti-HLA F antibody [EPR6803]

RabMAb
Recombinant
詳細を見る

(1 Review)

(4 Publications)

Rabbit Recombinant Monoclonal HLA F antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications.

別名を表示する

HLA-5.4, HLAF, HLA-F, CDA12, HLA F antigen, Leukocyte antigen F, MHC class I antigen F

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA F antibody [EPR6803] (AB126624)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA F antibody [EPR6803] (AB126624)

ab126624 at a 1/250 dilution staining HLA F in paraffin-embedded Human tonsil tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-HLA F antibody [EPR6803] (AB126624)

All lanes:

Western blot - Anti-HLA F antibody [EPR6803] (ab126624) at 1/1000 dilution

Lane 1:

HT1376 cell lysate at 10 µg

Lane 2:

Human tonsil lysate at 10 µg

Lane 3:

JAR cell lysate at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 39 kDa

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OI-RD Scanning - Anti-HLA F antibody [EPR6803] (AB126624)

OI-RD Scanning

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OI-RD Scanning - Anti-HLA F antibody [EPR6803] (AB126624)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR6803

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Stable for 12 months at -20°C

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Non-classical major histocompatibility class Ib molecule postulated to play a role in immune surveillance, immune tolerance and inflammation. Functions in two forms, as a heterotrimeric complex with B2M/beta-2 microglobulin and a peptide (peptide-bound HLA-F-B2M) and as an open conformer (OC) devoid of peptide and B2M (peptide-free OC). In complex with B2M, presents non-canonical self-peptides carrying post-translational modifications, particularly phosphorylated self-peptides. Peptide-bound HLA-F-B2M acts as a ligand for LILRB1 inhibitory receptor, a major player in maternal-fetal tolerance. Peptide-free OC acts as a ligand for KIR3DS1 and KIR3DL2 receptors (PubMed : 28636952). Upon interaction with activating KIR3DS1 receptor on NK cells, triggers NK cell degranulation and anti-viral cytokine production (PubMed : 27455421). Through interaction with KIR3DL2 receptor, inhibits NK and T cell effector functions (PubMed : 24018270). May interact with other MHC class I OCs to cross-present exogenous viral, tumor or minor histompatibility antigens to cytotoxic CD8+ T cells, triggering effector and memory responses (PubMed : 23851683). May play a role in inflammatory responses in the peripheral nervous system. Through interaction with KIR3DL2, may protect motor neurons from astrocyte-induced toxicity (PubMed : 26928464).

See full target information HLA-F

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1234577 PubMed37854606

2023

Single-cell profiling reveals immune disturbances landscape and HLA-F-mediated immune tolerance at the maternal-fetal interface in preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Fangyuan Luo,Fulin Liu,Yingzhe Guo,Wenming Xu,Yilin Li,Jun Yi,Thierry Fournier,Séverine Degrelle,Hedia Zitouni,Isabelle Hernandez,Xinghui Liu,Yu Huang,Jun Yue

Cells 12: PubMed36672208

2023

Silencing of circCRIM1 Drives IGF2BP1-Mediated NSCLC Immune Evasion.

Applications

Unspecified application

Species

Unspecified reactive species

Wenbei Peng,Linlin Ye,Qianqian Xue,Xiaoshan Wei,Zihao Wang,Xuan Xiang,Siyu Zhang,Pei Zhang,Haolei Wang,Qiong Zhou

Analytical cellular pathology (Amsterdam) 2018:7691704 PubMed30510890

2018

High HLA-F Expression Is a Poor Prognosis Factor in Patients with Nasopharyngeal Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Wu,Haihua Yang,Shenpeng Ying,Hongsheng Lu,Wei Wang,Jiaming Lv,Huacai Xiong,Wei Hu

American journal of reproductive immunology (New Y 78: PubMed28440588

2017

Endometrial immune markers are potential predictors of normal fertility and pregnancy after in vitro fertilization.

Applications

Unspecified application

Species

Unspecified reactive species

Louise Kofod,Anette Lindhard,Michael Bzorek,Jens Ole Eriksen,Lise Grupe Larsen,Thomas Vauvert F Hviid

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HLA F 抗体 [EPR6803]

Anti-HLA F antibody [EPR6803]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA F antibody [EPR6803] (AB126624)

Western blot - Anti-HLA F antibody [EPR6803] (AB126624)

OI-RD Scanning - Anti-HLA F antibody [EPR6803] (AB126624)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (4)

Abcam product promise